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Plasmid#16340DepositorInsertDsRed
UseTagsExpressionMammalianMutation5'Prime Site: Not I 3'Primer Site : No…PromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pF3BGX-TangoVL-lexA
Plasmid#138406PurposeRMCE vector for generating C-terminal Tango fusionDepositorInsertV2 tail-TEVcs(ENLYFQL)-LexAp65
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
CAG.3xHalo-GFP-SPC25
Plasmid#176206PurposeKinetochore anchor for TMP-Halo dimerization systemDepositorInsert3xHalo-GFP-SPC25
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSV2 neuNT
Plasmid#10918DepositorInsertneuNT (Erbb2 Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
TRX1 His C32S C35S
Plasmid#21286DepositorInsertTRX1 (TXN Human)
UseTagsHisExpressionMammalianMutationC32S C35SPromoterAvailable sinceJuly 30, 2009AvailabilityAcademic Institutions and Nonprofits only -
CAG.mCherry-eDHFR
Plasmid#176201PurposeNegative control of targeted optogenetic manipulation using TMP-Halo dimerization systemDepositorInsertmCherry-eDHFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCOND (Apr)
Plasmid#110094PurposeSuicide vector based on the TetR/Pip OFF system.DepositorTypeEmpty backboneUseBacterial knockout generationTagsExpressionMutationPromoterAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGR-BBa_B0010
Plasmid#46003DepositorInsertBBa_B0010 Terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceJune 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pRetroSuper Smad3
Plasmid#15726DepositorInsertSmad3 shRNA (SMAD3 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFBXC3GH
Plasmid#49051PurposeFX cloning insect cell expression vectorDepositorTypeEmpty backboneUseTags3C protease site (PreScission site), GFP, and dec…ExpressionInsectMutationPromoterpolH (polyhedrin)Available sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-HTB-kanMX6
Plasmid#26875DepositorInsertHTB tag
UsePcr tagging yeastTagsExpressionMutationPromoterAvailable sinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
Filamin-M-stFRET
Plasmid#61105PurposeExpression of Filamin with internal stFRET inserted to measure mechanical stress in real timeDepositorInsertfilamin-stFRET
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMEXNH3
Plasmid#110079PurposeAnalogous to pMINTNH3, but containing oriM for episomal replication in mycobacteria.DepositorTypeEmpty backboneUseTagsHis10 Tag - 3C cleavage siteExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
Actinin-M-stFRET
Plasmid#61103PurposeExpression of α-actinin with internal stFRET inserted to measure mechanical stress in real timeDepositorInsertα-actinin-stFRET
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pF3BGX-TangoVG-lexA
Plasmid#138405PurposeRMCE vector for generating C-terminal Tango fusionDepositorInsertV2 tail-TEVcs(ENLYFQG)-LexAp65
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CA-FLEX-RG
Plasmid#38043DepositorInsertRabies virus glycoprotein
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCAAvailable sinceAug. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRS406-PHO5-GFP-OSBP PH domain
Plasmid#58840PurposeExpresses GFP-Tagged OSBP PH domain in yeastDepositorInsertOxysterol-binding Protein PH domain (OSBP Human)
UseTagsGFPExpressionYeastMutationPromoterPHO5Available sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cas12c-sgRNA_BbsI_CAM
Plasmid#183221PurposeCas12c sgRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate AssemblyDepositorInsertCas12c single guide RNA containing BbsI sites in spacer sequence
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
FRB-polySH33
Plasmid#175233PurposeBacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycinDepositorInsertNCK1 (NCK1 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac GST-Plk4
Plasmid#80264Purposebaculovirus creation plasmid for GST-Plk4DepositorInsertPlk4 (PLK4 Human)
UseTagsGSTExpressionInsectMutationPromoterAvailable sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pHelper_O
Plasmid#173783PurposeThe 186 helper plasmid necessary for genomic integration of the pIT5_KO plasmid.DepositorInsert186 Integrase
UseSynthetic BiologyTagsExpressionBacterialMutationE59G - please see depositor commentsPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHelper_W
Plasmid#173784PurposeThe Wphi helper plasmid necessary for genomic integration of the pIT5_KW plasmid.DepositorInsertWphi Integrase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHelper_D
Plasmid#173781PurposeThe D145 helper plasmid necessary for genomic integration of the pIT5_KD plasmid.DepositorInsertD145 Integrase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Arch-EEQ
Plasmid#45188PurposeArch voltage indicator with double mutations D95Q-D106E (Arch-EEQ), with faster kinetics and greater fluorescence dynamic range than Arch-D95NDepositorInsertenhanced Archaerhodopsin
UseLentiviralTagseYFPExpressionMammalianMutationD95Q, D106EPromoterCamKIIaAvailable sinceJuly 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pF3BGX-TangoL-lexA
Plasmid#138404PurposeRMCE vector for generating C-terminal Tango fusionDepositorInsertTEVcs(ENLYFQL)-LexAp65
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-E2
Plasmid#175242PurposeBacterial expression and purification, E2 enzyme (UBC9) for SUMOylationDepositorInsertUBE2I (UBE2I Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pBB14
Plasmid#18657DepositorInsertUs9-EGFP gene fusion
UseTagsEGFPExpressionMammalianMutationFirst 106 amino acids of Us9 are intact. The stop…PromoterAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
p221z-AtMIR390a
Plasmid#118388PurposeEntry clone containing AtMIR390a backbone (split by ccdB gene). Used to make artificial microRNA construct. For use in plants and compatible with the MultiSite Gateway system.DepositorInsertAtMIR390a backbone
UseRNAiTagsExpressionMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUCSh3-mRFP
Plasmid#170983PurposeEncodes sgRNA scaffold with mRFP in place of target and Tetracycline resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.DepositorInsertmRFP
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLPCX mCER-Prx1
Plasmid#158227PurposeGenetically encoded HomoFRET-based probe to monitor peroxiredoxin1 (Prx1) oligomerization (for stable cell line generation)DepositorInsertmCER-Prx1
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLPCX mCER-Prx2
Plasmid#158225PurposeGenetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (for stable cell line generation)DepositorInsertmCER-Prx2
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD-iGECInano
Plasmid#186189PurposeExpression of small FRET-based near-infrared genetically-encoded calcium indicator in bacteriaDepositorInsertiGECInano
UseTagsExpressionBacterialMutationPromoterAraCAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pF3BGX-TangoG-lexA
Plasmid#138403PurposeRMCE vector for generating C-terminal Tango fusionDepositorInsertTEVcs(ENLYFQG)-LexAp65
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-HBH-hphMX4
Plasmid#26873DepositorInsertHBH-tag
UsePcr tagging yeastTagsExpressionMutationPromoterAvailable sinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
RAD Module G8-C1
Plasmid#38208DepositorInsertsSET Integrase
Bxb1 Excisonase
RESET Integrase
UseSynthetic Biology; Biobrick cloning vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPKC1 (JCE575)
Plasmid#89773Purposeyeast expression of PKC1DepositorInsertPKC1 (PKC1 Budding Yeast)
UseTagsExpressionYeastMutationPromoteryeast PKCAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-iGECInano
Plasmid#186188PurposeAAV vector packaging small FRET-based near-infrared genetically-encoded calcium indicatorDepositorInsertiGECInano
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-HBH-TRP1
Plasmid#26874DepositorInsertHBH tag
UsePcr tagging yeastTagsExpressionMutationPromoterAvailable sinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmp8a
Plasmid#163592PurposeExpresses BMP-8A in mammalian cellsDepositorInsertBmp8a (Bmp8a Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-HTB-TRP1
Plasmid#26877DepositorInsertHTB tag
UsePcr tagging yeastTagsExpressionMutationPromoterAvailable sinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCXN2-mVIN
Plasmid#11776DepositorInsertvinculin (Vcl Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pmMaple2-CAM
Plasmid#101149PurposeFluorescent protein for superresolution imagingDepositorInsertE. coli codon optimized mMaple2
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
Actinin-C-stFRET
Plasmid#61104PurposeExpression of α-actinin with C-term stFRET inserted to measure mechanical stress in real timeDepositorInsertα-actinin-stFRET
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSCW02
Plasmid#72301PurposeStarting substrate for in vitro DNA mismatch repair substrate monitored by FauI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.DepositorTypeEmpty backboneUseDerivative of puc19TagsExpressionBacterialMutationPromoterlacAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac GST-SAS6
Plasmid#80273Purposebaculovirus creation plasmid for GST-SAS6DepositorInsertSAS6 (SASS6 Human)
UseTagsGSTExpressionInsectMutationPromoterAvailable sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2-SP-FKBP-mCherry-TM-V5
Plasmid#186226PurposemRNA synthesis of rapalog-induced cell adhesion moleculeDepositorInsertSP-FKBP-mCherry-TM-V5
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-HBx NESM
Plasmid#24932DepositorInsertHBx
UseTagsEGFP and HAExpressionMammalianMutationL98A,G99A,L100APromoterAvailable sinceJune 10, 2010AvailabilityAcademic Institutions and Nonprofits only