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Showing: 1841 - 1880 of 156554 results

  2. pCasSA

    Plasmid
    #98211
    Purpose
    CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9
    Depositor
    Quanjiang Ji
    Article
    Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    98211-plasmid-map-sequence-id-327840

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  4. AAVS1-tdTomato

    Plasmid
    #159275
    Purpose
    Human AAVS1 targeting vector for knockin of a CAGGS promoter-driven tdTomato cassette (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.
    Depositor
    Rudolf Jaenisch
    Article
    Ma et al Proc Natl Acad Sci U S A. 2018 Apr 10;11
    Insert
    tdTomato
    Use
    AAV; S1 knockin donor vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAGGS
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    159275-plasmid-map-sequence-id-312908

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  7. TFORF0509

    Plasmid
    #143036
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TSHZ2 (TSHZ2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    143036-plasmid-map-sequence-id-385778

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  8. pLV-EGFP-Cre

    Plasmid
    #86805
    Purpose
    Lentiviral vector that expresses an EGFP-Cre fusion protein with a nuclear localization signal from the CMV promoter
    Depositor
    Amy Lasek
    Article
    Truitt et al eNeuro. 2016 Oct 31;3(5). pii: ENEURO.02
    Insert
    Cre recombinase
    Use
    Cre/Lox and Lentiviral
    Tags
    EGFP and nuclear localization signal: PKKKRKV
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    86805-plasmid-map-sequence-id-436570

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  11. TFORF0074

    Plasmid
    #142144
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    BACH2 (BACH2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    142144-plasmid-map-sequence-id-397722

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  16. pSynSRE-T-Luc

    Plasmid
    #60444
    Purpose
    Uses the hamster HMG-CoA synthase promoter sequence (-324/-225) and minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent transcriptional activation.
    Depositor
    Timothy Osborne
    Article
    Dooley et al J Biol Chem. 1998 Jan 16;273(3):1349-56.
    Insert
    HMG-CoA synthase promoter (-324/-225)
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    HMG-CoA synthase (-324/-225) & TATAA (-28/+39)
    Available since
    Feb. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    60444-plasmid-map-sequence-id-266122

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  18. pKL2299A

    Plasmid
    #222105
    Purpose
    A ternary vir helper plasmid that carries Agrobacterium tumefaciens virulence genes for enhanced plant genetic transformation.
    Depositor
    Kan Wang
    Article
    Aliu et al Front Plant Sci. 2024 Jul 23;15:1429353.
    Insert
    virA
    Use
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    virA promoter
    Available since
    Oct. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    222105-plasmid-map-sequence-id-442317

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  22. TFORF3052

    Plasmid
    #144528
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NCOA3 (NCOA3 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144528-plasmid-map-sequence-id-404217

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  24. pT4-CMV-GFP

    Plasmid
    #117046
    Purpose
    Optimized Sleeping Beauty Transposon Vector for GFP expression by a CMV promoter
    Depositor
    Wolfgang Uckert
    Insert
    GFP
    Use
    Transposon
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Oct. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    117046-plasmid-map-sequence-id-258678

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  25. pSupAR-MbPylRS(DiZPK)

    Plasmid
    #91705
    Purpose
    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interest
    Depositor
    P. Chen
    Article
    Zhang et al Nat Chem Biol. 2011 Sep 4;7(10):671-7. d
    Inserts
    pylS
    pylT
    Use
    Tags
    Expression
    Bacterial
    Mutation
    T810G, C813T, C820G, T821C, T937A, A1046T, G1257A
    Promoter
    Available since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    91705-plasmid-map-sequence-id-183278

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  27. pSecUAG-Evol2

    Plasmid
    #163148
    Purpose
    Expresses machinery necessary for selenocysteine incorporation at UAG codons
    Depositor
    Dieter Söll
    Article
    Mukai et al Angew Chem Int Ed Engl. 2018 Jun 11;57(2
    Inserts
    allo-tRNA UTu2D
    thioredoxin
    Selenophosphate synthase
    Selenocysteine synthase
    Cysteine desulfurase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Cysteine 32 switched to Selenocysteine (UAG), Cys…
    Promoter
    EM7, araBAD, native As SelD promoter, and native …
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    163148-plasmid-map-sequence-id-331409

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  33. pCMV-PE6c

    Plasmid
    #207853
    Purpose
    Mammalian expression of PE6c prime editor
    Depositor
    David Liu
    Article
    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.
    Insert
    PE6c
    Use
    Tags
    SV40 bpNLS and SV40 bpNLS, c-Myc NLS
    Expression
    Mammalian
    Mutation
    Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    207853-plasmid-map-sequence-id-423164

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  38. pKI_ΩBBMVP16&SRDXWUSm1

    Plasmid
    #220349
    Purpose
    Expresses BBM-VP16 and SRDX-WUSm1 in plant cells
    Depositor
    Tomoko Igawa
    Article
    Sato et al Front Plant Sci. 2024 Apr 3;15:1308417.
    Inserts
    BABY BOOM (BBM Mustard Weed)
    WUSCHEL (WUS Mustard Weed)
    Use
    Tags
    Superman Repression Domain X (SRDX) and VP16 tran…
    Expression
    Plant
    Mutation
    two amino acid substitution in WUS-box: L255A L25…
    Promoter
    CaMV 35S and RPS5A
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    220349-plasmid-map-sequence-id-440868

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Showing: 1841 - 1880 of 156554 results