Addgene

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pCellFree_G03 STAT6

Plasmid

#67123

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

STAT6 (STAT6 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 TFDP3

Plasmid

#67083

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TFDP3 (TFDP3 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 TFDP1

Plasmid

#67084

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TFDP1 (TFDP1 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 TFDP2

Plasmid

#67088

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TFDP2 (TFDP2 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pJZC102

Plasmid

#62337

Purpose

sgRNA for mammalian cells with mCherry marker

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbA5k-EPL14

Plasmid

#45405

Depositor

Aindrila Mukhopadhyay

Article

Dunlop et al Mol Syst Biol. 2011 May 10;7:487. doi: 1

Insert

mexF, PP_3426

Use

Synthetic Biology

Tags

His

Expression

Mutation

Promoter

LacUV5

Available since

Jan. 24, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbA5k-EPL11

Plasmid

#45403

Depositor

Aindrila Mukhopadhyay

Article

Dunlop et al Mol Syst Biol. 2011 May 10;7:487. doi: 1

Insert

ttgB, PP_1385

Use

Synthetic Biology

Tags

His

Expression

Mutation

Promoter

LacUV5

Available since

June 25, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pc3XB-ZF79

Plasmid

#13213

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF79

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pCellFree_G03 TFF1

Plasmid

#67047

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TFF1 (TFF1 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pc3XB-ZF9

Plasmid

#13108

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK_pro6_eGFP

Plasmid

#59851

Purpose

Constitutively expresses eGFP with pro6 promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

Enhanced Green Fluorescent Protein

Use

Tags

6x-His

Expression

Bacterial

Mutation

S2R,S65G,S72A

Promoter

pro6

Available since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pc3XB-ZF74

Plasmid

#13208

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF74

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pc3XB-ZF43

Plasmid

#13142

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF43

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pc3XB-ZF55

Plasmid

#13154

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF55

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pc3XB-ZF66

Plasmid

#13202

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF66

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pc3XB-ZF139

Plasmid

#13189

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF139

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK_proK14_eGFP

Plasmid

#59848

Purpose

Constitutively expresses eGFP with proK14 promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

Enhanced Green Fluorescent Protein

Use

Tags

6x-His

Expression

Bacterial

Mutation

S2R,S65G,S72A

Promoter

proK14

Available since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pc3XB-ZF59

Plasmid

#13194

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF59

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pc3XB-ZF69

Plasmid

#13205

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF69

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pc3XB-ZF93

Plasmid

#13227

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF93

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge