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Plasmid#112290PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) WW mutant. WQDP (199-202) in the first WW domain was changed to AQDA, and the WLDP (258-261) of the second WW domain was changed to ALDADepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationHuman YAP1-2gamma with mutations in the WW domain…PromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP(H66)
Plasmid#63558PurposeExpression of the Azure (blue) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(H66)
UseLentiviralTagsHis6 and T7ExpressionBacterial and MammalianMutationChanged tyrosine at position 66 with respect to t…PromoterCMV-EF1α hybrid (CEF)Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
TFORF0527
Plasmid#143038PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPAX7 (PAX7 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0104
Plasmid#141517PurposeLentiviral vector for overexpressing the SMAD9 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSMAD9 (SMAD9 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
dnCEBP: pLenti-TRE-A-C/EBP-PGK-rtTAA-2A-Puro
Plasmid#177201Purposeencoding a dominant negative form of CEBP under inducible promoterDepositorArticleInsertdnCEBP (A-C/EBP)
UseLentiviralTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
tre-stblHIF: pLenti-TRE-stblHIF-PGK-rtTAA-2A-Puro
Plasmid#177203Purposeencoding stblHIF under inducible promoterDepositorArticleInsertstblHIF
UseLentiviralTagsExpressionMammalianMutationP402A/P577G/N813APromoterTREAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR V2 sgACO1_1
Plasmid#169885PurposeDisrupt ACO1DepositorInsertsgRNA targeting ACO1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR V2 sgACO1_2
Plasmid#169892PurposeDisrput ACO1DepositorInsertsgRNA targeting ACO1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-PKMYT1-V5_IDG-K
Plasmid#135248PurposeGateway destination clone of PKMYT1 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertPKMYT1-V5 (PKMYT1 Human)
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER21-PGBD5
Plasmid#78121PurposeLentiviral gene expression vector for doxycycline inducible PGBD5 expressionDepositorInsertPGBD5 (PGBD5 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
GABPA_pLENTI-CAG-IRES-GFP
Plasmid#176999PurposeMammalian lentiviral expression vector encoding GABPADepositorInsertGABPA (GABPA Human)
UseLentiviralTagsExpressionMutationPromoterCAGAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-CMV-GFP-shDyn2
Plasmid#132712PurposeEncodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Pdyn geneDepositorInsertshDyn(2) (Pdyn Rat)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-p18INK4c
Plasmid#25650DepositorInsertp18INK4c (CDKN2C Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3/NS3
Plasmid#120841PurposeLentiviral expression of ZIKV proteinDepositorInsertNS3 ZIKV
UseLentiviralTagsV5ExpressionMutationPromoterCMVAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-DDX3X-E285K
Plasmid#116226PurposeLentiviral expression of DDX3X E285KDepositorInsertDDX3X (DDX3X Human)
UseLentiviralTagsExpressionMutationE285KPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO_HA.AU1.VSVg_NGFR
Plasmid#158232PurposeProtein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).DepositorInsertPro-Code Tagged human dNGFR (NGFR Human)
UseCRISPR and LentiviralTagsHA.AU1.VSVgExpressionMutationTruncation of the signaling domainPromoterEF1aAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiN 11E6
Plasmid#37448PurposeLentiviral expression of HPV 11E6 in mammalian cellsDepositorInsertHPV 11E6
UseLentiviralTagsFlag and HAExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
FUP95PDZGW (C5)
Plasmid#74021Purposelentiviral expression of Psd95-EGFP fusionDepositorInsertPsd95 (DLG4 Rat)
UseLentiviralTagsExpressionMammalianMutationPDZPromoterhUbCAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
ptcALPS gag-gfp Blasti
Plasmid#115806PurposeExpress gag-gfp fusion driven by TK promoterDepositorInsertgag-gfp
UseLentiviralTagsExpressionMutationPromoterTKAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRRL-VinculinTS-A50I
Plasmid#111831PurposeVinculin tension sensor with point mutation affecting ability to bind talin in lentiviral expression vectorDepositorInsertVinculinTS (A50I) (VCL Chicken)
UseLentiviralTagsExpressionMutationchanged vinculin alanine 50 to isoleucinePromoterCMVAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only