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pJSC236 - Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant
Plasmid#101235PurposeBacterial expression plasmid for SpCas9 Cluster 4 + Q926A variantDepositorInsertSpCas9 variant F491A/M495A/T496A/N497A/Q926A
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationF491A, M495A, T496A, N497A and Q926APromoterT7Available sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJSC239 - Bacterial expression plasmid for SpCas9 Cluster 3 + Q926A variant
Plasmid#101234PurposeBacterial expression plasmid for SpCas9 Cluster 3 + Q926A variantDepositorInsertSpCas9 variant T657A/G658A/W659A/R661A/Q926A
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationT657A, G658A, W659A, R661A and Q926APromoterT7Available sinceNov. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJSC269 - Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant
Plasmid#101220PurposeBacterial expression plasmid for SpCas9 Cluster 1 + Q926A variantDepositorInsertSpCas9 variant N692A/M694A/Q695A/H698A/Q926A
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationN692A, M694A, Q695A, H698A and Q926APromoterT7Available sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
p213-SWITCH-OFF
Plasmid#217888PurposeRetroviral Switch-OFF vector for sgRNA expression; U6-BbsIx2-SWITCH-OFF-scaffold; neoRDepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and RetroviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
p205-5'-Switch-ON
Plasmid#217884PurposeRetroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoRDepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and RetroviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
p204-U6-Switch-ON
Plasmid#217883PurposeRetroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoRDepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and RetroviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA020
Plasmid#216004PurposeFragmid fragment: (Cas protein) binds PP7 stem loopsDepositorInsertPP7 Core Protein (PCP)_v1.1
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRDB_162
Plasmid#216092PurposeCas9 [Sp] CRISPRi targeting CD47, positive controlDepositorInsertCD47 guide
UseCRISPR and Lentiviral; Positive controlsTagsExpressionMutationPromoterAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA299
Plasmid#216029PurposeFragmid fragment: (Cas protein) NNGG PAMDepositorInsertCas9_v1.1 [Slug]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA296
Plasmid#216028PurposeFragmid fragment: (Cas protein) NNNNCC PAMDepositorInsertNme2Cas9_v1.1 [Nme2]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA033
Plasmid#216013PurposeFragmid fragment: (Cas protein) deactivated CasDepositorInsertdCas9-NG_v1.1 [Sp]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA180
Plasmid#216023PurposeFragmid fragment: (Cas protein) NGRR(T) PAMDepositorInsertCas9_v1.1 [Sa]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA063
Plasmid#216020PurposeFragmid fragment: (Cas protein) nickase Cas for prime editingDepositorInsertnCas9-SpRY (H840A)_v1.1 [Sp]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA156
Plasmid#216022PurposeFragmid fragment: (Cas protein) Nanobody binds ALFA tagDepositorInsertNbALFA_v1.1
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA391
Plasmid#216033PurposeFragmid fragment: (Cas protein) reverse TetR; binds to TRE in presence of doxycyclineDepositorInsertrTetR_v1.1
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA303
Plasmid#216071PurposeFragmid fragment: (guide cassette) guide expressionDepositorInsertU6_v1; BsmBI_v0; BsmBI_v6; trRNA_v1 [Nme2]; terminator_v1
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA304
Plasmid#216072PurposeFragmid fragment: (guide cassette) guide expressionDepositorInsertU6_v1; BsmBI_v0; BsmBI_v1; trRNA_v1 [Sa]; terminator_v1
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only
