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pSYN-VWF-039
Plasmid#120292PurposeVWF expression construct expressing D'D3 domains with C1099A/C1142A mutations in D3 domain as an Fc fusion proteinDepositorInsertVWF D1-D2-D'-D3-Fc
UseTagsFcExpressionMammalianMutationC1099A/C1142APromoterCMVAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-NEW-fRGECO2
Plasmid#125248PurposeExpresses red fluorescent calcium sensor in mammalian cellsDepositorInsertfRGECO2
UseTagsNES signalExpressionMammalianMutationPromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
p148
Plasmid#62616PurposeePathBrick pETM6 vector encoding CsF3H, AaDFR, and DuLARDepositorInsertsflavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
UseSynthetic BiologyTagsGBD, PDZ, and SH3ExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti CRISPR V2 sgMTFMT_1
Plasmid#106318PurposeExpress Cas9 and sgRNA targeting MTFMTDepositorInsertsgRNA targeting MTFMT
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET11-PC-H6
Plasmid#183777PurposeExpress PC-H6 in E. coli. Purified PC-H6 was used for for generating antibodies in rabbits and for GST-CBP pull-down assays.DepositorInsertPolycomb (Pc Fly)
UseTags6 His tag at the C-terminusExpressionBacterialMutationPromoterT7Available sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
mcherry Beta 3
Plasmid#36113DepositorInsertG protein beta subunit B3 (GNB3 Human)
UseTagsmcherryExpressionMammalianMutationPromotercmvAvailable sinceMay 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSilencer2.1-U6-3JB1R+6
Plasmid#190842PurposeFor mammalian expression of 3JB1R+6, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.DepositorInsert3JB1R_AP3+6bp (A Dual-Specificity aptamer targeting OGT and GFP)
UseAffinity Reagent/ Antibody and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFA6a-GFP (F64L, S65T, V163A)-TRP1
Plasmid#53184Purposechromosomal tagging with stable GFPDepositorInsertTRP1 (TRP1 Budding Yeast)
UseYeast genomic targetingTagsGFP (F64L, S65T, V163A)ExpressionMutationPromoterAvailable sinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET23a-MmRPS27a
Plasmid#69559PurposeBacterial expression; in vitro transcription/translation (T7)DepositorInsertRPS27A (RPS27A Mouse, Human)
UseTagsExpressionBacterialMutationChanged Alanine 133 (human RPS27a) to Glycine (mo…PromoterT7Available sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
mcherry Beta 4
Plasmid#36114DepositorInsertG protein beta subunit B4 (Gnb4 Mouse)
UseTagsmcherryExpressionMammalianMutationPromotercmvAvailable sinceMay 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV DCC mut
Plasmid#16460DepositorInsertDCC (DCC Human)
UseTagsExpressionMammalianMutation5' end of DCCPromoterAvailable sinceMarch 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046925
Plasmid#145694PurposeBacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;StrepIIExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEPQD1CB0817
Plasmid#177039PurposeMoClo Level 1, position 1, transcriptional unit for transient expression of truncated 3-hydroxy-3-methylglutaryl-coenzyme A reductase (AstHMGR1) from Avena strigosa driven by 35S promoterDepositorInserttruncated 3-hydroxy-3-methylglutaryl-coenzyme A reductase (AstHMGR1) from Avena strigosa
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUAST-attB-Flag-dTSC2 WT
Plasmid#111807PurposeInsect Expression of dTSC2DepositorInsertdTSC2 (gig Fly)
UseTags3xFlagExpressionInsectMutationPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-V5-Vrk2
Plasmid#120240PurposeExpresses V5-tagged Vrk2 in lentiviral vectorDepositorInsertVrk2 (Vrk2 Mouse)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGs-Hoxc8
Plasmid#45555DepositorInsertHoxc8 (HOXC8 Chicken)
UseTagsExpressionMutationPromoterCMV / b-actinAvailable sinceAug. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pZS*11_4enh
Plasmid#149423PurposePlasmid harboring four plant enhancers (35S, AB80, Cab-1, and rbcS-E9).DepositorInsertsrbcS-E9 enhancer
35S core enhancer
Cab-1 enhancer
AB80 enhancer
UseTagsT120>AExpressionBacterialMutationPromoterAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPKm-118
Plasmid#90491PurposeExpresses pcDNA3 - CMVmin - 5X UAS - pFR LuciferaseDepositorInsertpFR Luciferase Fluc
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-H2A-Akt-STOPS
Plasmid#175010PurposeAkt-STOPS targeted to the nucleus via histone H2A.DepositorInsertH2A-mCherry-Akt-STOPS
UseTagsHistone H2A and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only
