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Plasmid#199587PurposeContains guide RNA to 5' end of mouse EZH2 geneDepositorInsertEZH2 (Ezh2 Mouse)
UseCRISPR and Mouse TargetingTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330_EZH2gRNA16
Plasmid#199588PurposeContains guide RNA to 3' end of mouse EZH2 geneDepositorInsertEZH2 (Ezh2 Mouse)
UseCRISPR and Mouse TargetingTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330_EZH2gRNA17
Plasmid#199589PurposeContains guide RNA to 3' end of mouse EZH2 geneDepositorInsertEZH2 (Ezh2 Mouse)
UseCRISPR and Mouse TargetingTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330_EZH2gRNA14
Plasmid#199586PurposeContains guide RNA to 5' end of mouse EZH2 geneDepositorInsertEZH2 (Ezh2 Mouse)
UseCRISPR and Mouse TargetingTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hNRG1a
Plasmid#199235PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertNeuregulin (NRG1 Human)
UseTagsTrxA-6xHis-S-tagExpressionBacterialMutationPromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1-5xmut-dsRNAres_U
Plasmid#147707PurposeInsect Expression of DmNot1-5xmut-dsRNAresDepositorInsertDmNot1-5xmut-dsRNAres (Not1 Fly)
UseTagsExpressionInsectMutation10 silent mutations and two non silent N605S, K75…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1-6x mut-dsRNAres_U
Plasmid#147708PurposeInsect Expression of DmNot1-6xmut-dsRNAresDepositorInsertDmNot1-6xmut-dsRNAres (Not1 Fly)
UseTagsExpressionInsectMutation10 silent mutations and two non silent N605S, K75…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1-7xmut-dsRNAres_U
Plasmid#147709PurposeInsect Expression of DmNot1-7xmut-dsRNAresDepositorInsertDmNot1-7xmut-dsRNAres (Not1 Fly)
UseTagsExpressionInsectMutation10 silent mutations and three non silent N605S, K…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1-8xmut-dsRNAres_U
Plasmid#147710PurposeInsect Expression of DmNot1-8xmut-dsRNAresDepositorInsertDmNot1-8xmut-dsRNAres (Not1 Fly)
UseTagsExpressionInsectMutation10 silent mutations and three non silent N605S, K…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1_1147-1717-K1277A_R
Plasmid#147431PurposeInsect Expression of DmNot1_1147-1717-K1277ADepositorInsertDmNot1_1147-1717-K1277A (Not1 Fly)
UseTagsExpressionInsectMutation2 non silent mutations compared to the sequence g…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1_1149-2505-dsRNAres_R
Plasmid#147432PurposeInsect Expression of DmNot1_1149-2505-dsRNAresDepositorInsertDmNot1_1149-2505-dsRNAres (Not1 Fly)
UseTagsExpressionInsectMutation6 silent mutations compared to the sequence given…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpG-DmNOT1_1-1083_R
Plasmid#147441PurposeBacterial Expression of DmNot1_1-1083DepositorInsertDmNot1_1-1083 (Not1 Fly)
UseTagsExpressionBacterialMutation4 silent mutations and two non silent N605S, K759…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_1-226_R
Plasmid#147416PurposeInsect Expression of DmNot1_1-226DepositorInsertDmNot1_1-226 (Not1 Fly)
UseTagsExpressionInsectMutation1 silent mutation compared to the sequence given …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_1-412_R
Plasmid#147417PurposeInsect Expression of DmNot1_1-412DepositorInsertDmNot1_1-412 (Not1 Fly)
UseTagsExpressionInsectMutation1 silent mutation compared to the sequence given …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_416-1148_R
Plasmid#147418PurposeInsect Expression of DmNot1_416-1148DepositorInsertDmNot1_416-1148 (Not1 Fly)
UseTagsExpressionInsectMutation3 silent and two non silent N605S, K759M mutation…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-E798AQ800A_M
Plasmid#146952PurposeInsect Expression of DmAGO1-E798AQ800ADepositorInsertDmAGO1-E798AQ800A (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations V315A and H…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-E798QQ800E_M
Plasmid#146953PurposeInsect Expression of DmAGO1-E798QQ800EDepositorInsertDmAGO1-E798QQ800E (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations V315A and H…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-H941A_M
Plasmid#146954PurposeInsect Expression of DmAGO1-H941ADepositorInsertDmAGO1-H941A (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations V315A and H…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-H941K_M
Plasmid#146955PurposeInsect Expression of DmAGO1-H941KDepositorInsertDmAGO1-H941K (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations V315A and H…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-H941Q_M
Plasmid#146956PurposeInsect Expression of DmAGO1-H941QDepositorInsertDmAGO1-H941Q (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations V315A and H…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only