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TMEM16F-I521K-peGFP-N1

Plasmid

#228588

Purpose

Expresses TMEM16F-I521K-peGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

I521K plus a 3 amino acid N-terminal truncation (…

Promoter

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.26-Ccnd2PromoterRegion1(1.2kb)

Plasmid

#228058

Purpose

1.2 kb from mouse Ccnd2 promoter in front of firefly luciferase reporter

Depositor

Kenneth Chen

Article

Fraire et al bioRxiv [Preprint]. 2024 Apr 30:2024.04.

Insert

Ccnd2 promoter (Ccnd2 Mouse)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.26-Ccnd2PromoterRegion2(0.5kb)

Plasmid

#228059

Purpose

0.5 kb from mouse Ccnd2 promoter in front of firefly luciferase reporter

Depositor

Kenneth Chen

Article

Fraire et al bioRxiv [Preprint]. 2024 Apr 30:2024.04.

Insert

Ccnd2 promoter (Ccnd2 Mouse)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.26-Igf2Promoter

Plasmid

#228060

Purpose

0.9 kb from mouse Igf2 promoter in front of firefly luciferase reporter

Depositor

Kenneth Chen

Article

Fraire et al bioRxiv [Preprint]. 2024 Apr 30:2024.04.

Insert

Igf2 promoter (Igf2 Mouse)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCF153_Gag-Pol

Plasmid

#225961

Purpose

CMV-Intron-GagPol (FMLV). Expresses FMLV (Friend Murine Leukemia Virus) Gag-Pol for VLP production.

Depositor

Christof Fellmann

Article

Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

Insert

CMV-Intron-GagPol (FMLV)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pCF142_U6-sgRNA

Plasmid

#225960

Purpose

U6-sgRNA (recipient). Expresses U6-sgRNA (recipient) for VLP production. This is a recipient vector for cloning of specific SpyCas9 sgRNAs.

Depositor

Christof Fellmann

Article

Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

Insert

U6-sgRNA (recipient)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pCKC014

Plasmid

#226623

Purpose

pLenti wt hTdT template for library 3

Depositor

Chang Liu

Article

Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

Insert

Terminal deoxynucleotidyl transferase (DNTT Human)

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-rTetR(SE)-EED-2A-mTurquoise

Plasmid

#225692

Purpose

Lentiviral expression of rTetR(SE) fused to EED and mTurquoise-NLS

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

rTetR-EED (EED Human, Synthetic)

Use

Lentiviral and Synthetic Biology

Tags

3xFLAG and T2A-mTurquoise

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-rTetR(SE)-HP1a-2A-mTurquoise

Plasmid

#225691

Purpose

Lentiviral expression of rTetR(SE) fused to HP1a and mTurquoise-NLS

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

HP1a (CBX5 Human, Synthetic)

Use

Lentiviral and Synthetic Biology

Tags

3xFLAG and T2A-mTurquoise

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-3*flag-NEXN

Plasmid

#216834

Purpose

expressing Falg tag and the coding sequence of mouse Nexn

Depositor

Yuxuan Guo

Article

Guo et al Acta Pharmacol Sin. 2024 Jul 23. doi: 10

Insert

Nexn (mus musculus) (Nexn Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-eSpCas9-2A-GFP-sgRNA-Lig4

Plasmid

#220494

Purpose

To generate LIG4 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against LIG4 exon 3.

Depositor

Sébastien Britton

Article

Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

Insert

sgRNA targeting LIG4 exon 3 (LIG4 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pOTTC1642 - pAAV EF1a EGFP-KASH with mTACR1 gRNAs

Plasmid

#195018

Purpose

An adeno-associated viral vector expressing eGFP-KASH and two guides targeting mouse TACR1

Depositor

Christopher Richie

Article

Belilos et al bioRxiv [Preprint]. 2023 Oct 9:2023.02.0

Inserts

CCGTATAGGCGGCTGCCCAA
EGFP-KASH
TTCCGTGGTGGGCAACGTAG

Use

AAV

Tags

KASH domain of Nesprin2

Expression

Mutation

Promoter

EF1a, hU6, and mU6

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-APOBEC1-YTHmut-EGFP

Plasmid

#209323

Purpose

AAV packaging vector containing a APOBEC1-YTHmut expression cassette, a P2A-EGFP expression cassette.

Depositor

Magdalena Koziol

Article

Feng et al Genome Res. 2024 Jul 23;34(6):822-836. d

Insert

APOBEC1--YTHmut-HA

Use

AAV

Tags

HA

Expression

Mutation

YTH domain lacks AA 385-409 comprising the m6A-bi…

Promoter

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Zmynd8-exon minimal CMV pCDNA5

Plasmid

#212078

Purpose

LR vector for integration of Zmynd8-exon into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Zmynd8-exon (Zmynd8 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Zmynd8+exon minimal CMV pCDNA5

Plasmid

#212079

Purpose

LR vector for integration of Zmynd8+exon(48nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Zmynd8+exon(48nt) (Zmynd8 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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dCas9-RPT6-FLAG

Plasmid

#205416

Purpose

Expresses dCas9-RPT6 with FLAG label

Depositor

Timothy Jarome

Article

Farrell et al J Neurosci. 2024 Jan 24;44(4):e145323202

Insert

dCas9-RPT6 (Psmc5 Rat)

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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GFP Cav2.2 W391A pRK5

Plasmid

#206084

Purpose

expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2 that prevents Beta subunit binding

Depositor

Annette Dolphin

Article

Waithe et al J Biol Chem. 2011 Mar 18;286(11):9598-61

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

GFP

Expression

Mammalian

Mutation

W391A

Promoter

CMV

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 HA W391A pcDNA3

Plasmid

#206071

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2

Depositor

Annette Dolphin

Article

Waithe et al J Biol Chem. 2011 Mar 18;286(11):9598-61

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

W391A

Promoter

CMV

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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CAGGS-SAFB-NLS-3XFLAG-V5

Plasmid

#196089

Purpose

Expresses mouse SAFB in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

Depositor

Mauro Calabrese

Article

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Insert

SAFB (Safb Mouse)

Use

Tags

C-terminal 3xFLAG and C-terminal V5

Expression

Bacterial and Mammalian

Mutation

Promoter

CAGGS

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pX330_SAFBgRNA8

Plasmid

#196103

Purpose

Contains guide RNA to 3' end of mouse SAFB gene for safb1/2 dko. Used with Addgene IDs: 196106, 196107, 196108

Depositor

Mauro Calabrese

Article

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Insert

SAFB (Safb Mouse)

Use

CRISPR and Mouse Targeting

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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