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HL 2304 (=HL 716 + pHL 269)

Plasmid

#30019

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLtetO-1:MicC-mCherry, pLlacO-1:ompC::gfp

Use

Synthetic Biology

Tags

mCherry, GFP

Expression

Bacterial

Available Since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only
HL 2300 (=HL 716 + pHL 745)

Plasmid

#30017

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLtetO-1:RhyB-mCherry, pLlacO-1:sodB::gfp

Use

Synthetic Biology

Tags

mCherry, GFP

Expression

Bacterial

Available Since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only
HL 1178 (=HL 862 + pHL 282)

Plasmid

#30012

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLtetO-1:mCherry, pLlacO-1:ompC::gfp

Use

Synthetic Biology

Tags

mCherry, GFP

Expression

Bacterial

Available Since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only
HL 4178 (=HL 862 + pHL 1360)

Plasmid

#30097

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLlacO-1:hfq, pLtetO-1:ompC::gfp

Use

Synthetic Biology

Tags

GFP

Expression

Bacterial

Available Since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only
HL 1329 (=HL 865 + pHL 344)

Plasmid

#30014

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLtetO-1:mCherry, pLlacO-1:rpoS::gfp

Use

Synthetic Biology

Tags

mCherry, GFP

Expression

Bacterial

Available Since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only
HL 2314 (=HL 716 + pHL 818)

Plasmid

#30025

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLtetO-1:MicC, pLtetO-1:fhlA::mCherry, pLlacO-1:ompC::gfp

Use

Synthetic Biology

Tags

mCherry, GFP

Expression

Bacterial

Available Since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only
HL 2625 (=HL 716 + pHL 908)

Plasmid

#30028

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLlacO-1:RhyB::mCherry, pLtetO-1m9:sodB::gfp

Use

Synthetic Biology

Tags

mCherry, GFP

Expression

Bacterial

Available Since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only
HL 3612 (=HL 716 + pHL 841)

Plasmid

#30082

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLtetO-1:RhyB-mCherry, pLlacO-1:ompC::gfp

Use

Synthetic Biology

Tags

mCherry, GFP

Expression

Bacterial

Available Since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only
HL 3619 (=HL 716 + pHL 1228)

Plasmid

#30083

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLtetO-1:DsrA-mCherry, pLlacO-1:ompC::gfp

Use

Synthetic Biology

Tags

mCherry, GFP

Expression

Bacterial

Available Since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only
pLP-LNCX-PIK3CA-W11STOP

Plasmid

#25634

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

Insert

PIK3CA (PIK3CA Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only
pLVX-TetOne-bleo-atpD-K155Q

Plasmid

#248068

Purpose

Lentiviral expression of a catalytically inactive mutant of the beta subunit of ATPGobble. Use together with pLVX-TetOne-bsd-atpA (#248065) and pLVX-TetOne-puro-atpG (#248067) for a LOF control.

Depositor

Denis Titov

Article

Ekvik et al bioRxiv [Preprint]. 2025 Aug 23:2025.08.

Insert

atpD

Use

Lentiviral

Tags

FLAG

Mutation

Lysine 155 to Glutamine, codon-optimized for expr…

Promoter

TRE3GS

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-bleo-atpD

Plasmid

#248066

Purpose

Lentiviral expression of beta subunit of ATPGobble. Includes ampR and bleoR (zeocin). Use together with pLVX-TetOne-bsd-atpA (#248065) and pLVX-TetOne-puro-atpG (#248067) for active ATPGobble.

Depositor

Denis Titov

Article

Ekvik et al bioRxiv [Preprint]. 2025 Aug 23:2025.08.

Insert

atpD

Use

Lentiviral

Tags

FLAG

Mutation

codon-optimized for expression in human cells

Promoter

TRE3GS

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-bsd-atpA

Plasmid

#248065

Purpose

Lentiviral expression of alpha subunit of ATPGobble. Includes ampR and bsdR (blasticidin). Use together with pLVX-TetOne-bsd-atpD (#248066) and pLVX-TetOne-atpG (#248067) for active ATPGobble.

Depositor

Denis Titov

Article

Ekvik et al bioRxiv [Preprint]. 2025 Aug 23:2025.08.

Insert

atpA

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

codon-optimized for expression in human cells

Promoter

TRE3GS

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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sortase A pentamutant (eSrtA) in pET29

Plasmid

#75144

Purpose

evolved sortase A (eSrtA) pentamutant with improved kinetics and activity

Depositor

David Liu

Article

Chen et al Proc Natl Acad Sci U S A. 2011 Jul 12;10

Insert

sortase A pentamutant

Tags

6x His

Expression

Bacterial

Mutation

P94R, D160N, D165A, K190E, K196T. Deleted amino a…

Promoter

T7

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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VR124 pETv2 10His-pG-Tn5 (E54K, L372P)

Plasmid

#198468

Purpose

E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in T7 Express lysY/Iq protein expression strain.

Depositor

Viviana Risca

Article

Soroczynski et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

Insert

10His-pG-Tn5 (E54K, L372P)

Tags

10His-pG

Expression

Bacterial

Mutation

E54K, L372P

Promoter

Consensus φ10 T7 Promoter

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCW57.1-4EBP1_4xAla

Plasmid

#38240

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

4EBP1 (Eif4ebp1 Rat, hybrid gene of rat and human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Mutation

T37A, T46A, S65A, T70A (dominant negative)

Promoter

Tet ON

Available Since

Nov. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pUCXMG

Plasmid

#204409

Purpose

Expression plasmid for cloning of FatI digested metagenomic DNA into a compatible NcoI site

Depositor

David Ackerley

Article

Rich et al bioRxiv. 2023 Mar 25:2023.03.24.534183.

Type

Empty backbone

Tags

His6

Expression

Bacterial

Promoter

tac

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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SG3ΔENV CA E45A

Plasmid

#145783

Purpose

Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and E45A mutation in capsid (CA E45A).

Depositor

Wesley Sundquist

Article

Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/

Insert

Capsid

Use

Lentiviral and Retroviral

Expression

Mammalian

Mutation

Changed Capsid residue glutamic acid 45 to alanin…

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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SG3ΔENV RT D185A

Plasmid

#145782

Purpose

Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and D185A mutation in reverse transcriptase (RT D185A).

Depositor

Wesley Sundquist

Article

Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/

Insert

Reverse Transcriptase

Use

Lentiviral and Retroviral

Expression

Mammalian

Mutation

Changed Reverse Transcriptase Aspartic Acid 185 t…

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pFlox_N-p300_BSD_FKBPF36V_EYFP

Plasmid

#163885

Purpose

Acute Depletion P300-EYFP

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

P300 (Ep300 Mouse)

Expression

Mammalian

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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