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Plasmid#134264PurposeLentivector encoding Flag-tagged GK5 truncation (amino acids 287-534)DepositorInsertGk5 (Gk5 Mouse)
UseLentiviralTagsFlagExpressionMammalianMutationmouse Gk5 amino acids 287-534PromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBOB-C-Flag-GK5 (25-287)
Plasmid#134263PurposeLentivector encoding Flag-tagged GK5 truncation (amino acids 25-287)DepositorInsertGk5 (Gk5 Mouse)
UseLentiviralTagsFlagExpressionMammalianMutationmouse Gk5 amino acids 25-287PromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBOB-C-Flag-GK5 (1-287)
Plasmid#134262PurposeLentivector encoding Flag-tagged GK5 truncation (amino acids 1-287)DepositorInsertGk5 (Gk5 Mouse)
UseLentiviralTagsFlagExpressionMammalianMutationmouse Gk5 amino acids 1-287PromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBOB-C-Flag-GK5 (287-396)
Plasmid#134265PurposeLentivector encoding Flag-tagged GK5 truncation (amino acids 287-396)DepositorInsertGk5 (Gk5 Mouse)
UseLentiviralTagsFlagExpressionMammalianMutationmouse Gk5 amino acids 287-396PromoterCMVAvailable sinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
MBP-rPKL S12TAG
Plasmid#107155PurposeMBP-rPKL construct for production and purification of rat PKL pS12DepositorInsertMBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
UseTagsC-term 6-his and N-term MBPExpressionBacterialMutationSerine 12 to TAG for phosphoserine insertionPromoterPBADAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
MBP-rPKL S113TAG
Plasmid#107157PurposeMBP-rPKL S113TAG for production and purification of rat PKL pS113DepositorInsertMBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
UseTagsC-term 6-his and N-term MBPExpressionBacterialMutationSerine 113 changed to TAG for phosphoserine inser…PromoterPBADAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
Anti-Ataxin-1, 11NQ (Mouse) [N76/8R]
Plasmid#128641PurposeMammalian Expression Plasmid of anti-Ataxin-1, 11NQ (Mouse). Derived from hybridoma N76/8.DepositorInsertanti-Ataxin-1, 11NQ (Mouse) recombinant mouse monoclonal antibody (Atxn1 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
Anti-DLX1 [L43/40R]
Plasmid#128633PurposeMammalian Expression Plasmid of anti-DLX1 (Human). Derived from hybridoma L43/40.DepositorInsertanti-DLX1 (Human) recombinant mouse monoclonal antibody (DLX1 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
Anti-KCNT2/Slo2.1/Slick K+ channel [N11/33R]
Plasmid#114496PurposeMammalian Expression Plasmid of anti-KCNT2/Slo2.1/Slick K+ channel (Mouse). Derived from hybridoma N11/33.DepositorInsertanti-KCNT2/Slo2.1/Slick K+ channel (Mus musculus) recombinant mouse monoclonal antibody (Kcnt2 Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
Anti-MESD [N88/12.1R]
Plasmid#114539PurposeMammalian Expression Plasmid of anti-MESD (Mouse). Derived from hybridoma N88/12.1.DepositorInsertanti-MESD (Mus musculus) recombinant mouse monoclonal antibody (Mesd Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
Anti-Botch [N116/14R]
Plasmid#114487PurposeMammalian Expression Plasmid of anti-Botch (Rat). Derived from hybridoma N116/14.DepositorInsertanti-Botch (Rattus norvegicus) recombinant mouse monoclonal antibody (Chac1 Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Anti-Clcn4 [N222/6R]
Plasmid#114474PurposeMammalian Expression Plasmid of anti-Clcn4 (Human). Derived from hybridoma N222/6.DepositorInsertanti-Clcn4 (Homo sapiens) recombinant mouse monoclonal antibody (CLCN4 Mouse)
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEAHISSOarD1
Plasmid#97067PurposeExpressing Sulfolobus solfataricus orf Sso Ard1 (N-terminal acetylase) proteinDepositorInsertS. solfataricus Sso Ard1 protein
UseTagsN-terminal TEV protease cleavable 6xHis tagExpressionMutationnonePromoterT7Available sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cer(1-158)-beta-1 in pcDNAI/Amp
Plasmid#55707PurposeAn amino-terminal fragment of mCerulean was fused to Gbeta1. When co-expressed with a carboxyl terminal CFP fragment fused to a Ggamma subunit, a fluorescent signal s produced.DepositorInsertmCer(1-158)-beta 1 (GNB1 Human, Aequorea victoria)
UseTagsCer(1-158) was fused to the amino terminus of Gbe…ExpressionMammalianMutationGBeta-1 was amplified via PCR, which removed an i…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cer(159-238)-beta-5 in pcDNAI/Amp
Plasmid#55778PurposeA carboxyl-terminal mCerulean fragment was fused to Gbeta-5. When co-expressed with an amino terminal mCerulean fragment fused to a Ggamma subunit, a fluorescent signal is produced.DepositorInsertCer(159-238)-beta-5 (GNB5 Human, Aequorea victoria)
UseTagsmCer(159-238) was fused to the amino terminus of …ExpressionMammalianMutationGBeta-5 was amplified via PCR, which added an N-t…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST26 Ago2 4A
Plasmid#59907PurposeExpresses 6xHis-Ago2 (N-termi tag) mutated four serines to alanines in mammalian cellsDepositorInsertArgonaute 2 (AGO2 Human)
UseTags6xHis (N-termi tag)ExpressionMammalianMutationmutated four serines to alaninesPromoterCMVAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
HRas (G12V, E37G)-pcw107
Plasmid#64639Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertHRAS (transcript variant 3) (HRAS Human)
UseLentiviralTagsExpressionMutationG12V, E37GPromoterPGKAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
HRas (G12V, E37G)-pcw107-V5
Plasmid#64640Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertHRAS (transcript variant 3) (HRAS Human)
UseLentiviralTagsV5ExpressionMutationG12V, E37GPromoterPGKAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR D-TOPO-C2_8
Plasmid#60249PurposeThis plasmid contains a human pancreatic islet inactive enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.DepositorInsertRAP1A inactive enhancer (RAP1A Human)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR D-TOPO-C2_11
Plasmid#60251PurposeThis plasmid contains a human pancreatic islet inactive enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.DepositorInsertSMYD3 inactive enhancer (SMYD3 Human)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only