-
Plasmid#120245PurposeExpresses V5-tagged Lem2 in lentiviral vectorDepositorInsertLem2 (Lemd2 Mouse)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQCas12j2
Plasmid#189780PurposeGateway entry plasmid (attL1 & attR5) expressing NLS-Cas12j2-NLS, without promoterDepositorInsertCas12j2
UseCRISPR; Gateway compatible cas12j2 entry cloneTagsNLSExpressionPlantMutationPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-Cas12j2
Plasmid#173924PurposeGateway entry plasmid (attL1 & attR5) expressing NLS-Cas12j2-NLSDepositorInsertNLS-Cas12j2-NLS
UseCRISPRTagsNLSExpressionPlantMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mGold-MannII-N-10
Plasmid#158005PurposeVisualization of Golgi in mammalian cells using mGoldDepositorInsertMannII (Man2a1 Mouse)
UseTagsmGoldExpressionMammalianMutationInsert contains only aa 1-112 of MannIIPromoterCMVAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pv6_CBX7_Chromo_TAF3_Phd
Plasmid#179400PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo - TAF3_PhdDepositorInsertCBX7_Chromo - TAF3_Phd
UseTagsAviTag and EGFPExpressionMammalianMutationPromoterCAGGSAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Stop-EGFP (MSE)
Plasmid#154469PurposeMSCV-Stop-EGFP-pgk-Neo (retroviral construct containing the RFP-based translational stop cassette)DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P POLE1_2
Plasmid#160764PurposeSuppress POLE1DepositorInsertshPOLE1_2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET48 AtGUN1-SMR1
Plasmid#136361PurposeBacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 709 to 918)DepositorInsertcDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 709 to 918 (GUN1 Mustard Weed)
UseTagsTRX-HisExpressionBacterialMutationPromoterT7Available sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET48 AtGUN1-SMR2
Plasmid#136362PurposeBacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 781 to 918)DepositorInsertcDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 781 to 918 (GUN1 Mustard Weed)
UseTagsTRX-HisExpressionBacterialMutationPromoterT7Available sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET48 AtGUN1-PPR1
Plasmid#136359PurposeBacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 708)DepositorInsertcDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 708 (GUN1 Mustard Weed)
UseTagsTRX-HisExpressionBacterialMutationPromoterT7Available sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET48 AtGUN1-PS
Plasmid#136358PurposeBacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 918)DepositorInsertcDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 918 (GUN1 Mustard Weed)
UseTagsTRX-HisExpressionBacterialMutationPromoterT7Available sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET48 AtGUN1-PPR2
Plasmid#136360PurposeBacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 619)DepositorInsertcDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 619 (GUN1 Mustard Weed)
UseTagsTRX-HisExpressionBacterialMutationPromoterT7Available sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH-3xFLAG-hTCAB1-puroR
Plasmid#167459Purpose3rd-gen lentiviral expression of FLAG-TCAB1 (WDR79) in mammalian cellsDepositorInsertTCAB1 (WRAP53 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sa-SlugCas9
Plasmid#163797PurposeExpresses Sa-SlugCas9, and cloning backbone for sgRNADepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjFOXP2-hum
Plasmid#129757PurposeGene targeting in marmoset cellsDepositorInsertFOXP2 (LOC100411307 C. jacchus (common marmost))
UseMarmoset targetingTagsExpressionMutationT301N/N323SPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
XLone-BSD Cas13d P2A mCherry
Plasmid#155183PurposePiggybacTransposon-based tunable and temporal expression control of Cas13d and mCherryDepositorInsertCasRx
UseUnspecified; Piggybac transposonTagsNLS and P2A mCherryExpressionMammalianMutationPromoterTRE3GAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_No_FLAG_ATP1A1_G2
Plasmid#86610PurposeExpresses the ATP1A1 G2 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 exon 4. Px330-like plasmidDepositorInsertATP1A1 G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
UseCRISPR; Co-selection via nhej using ouabainTagsExpressionMammalianMutationK848A, K1003A, & R1060APromoterCBhAvailable sinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2833 pPB: CAG-PYL1-VPR-IRES-Zeo-WPRE-SV40PA PGK-ABI-tagBFP-SadCas9
Plasmid#84243PurposeExpresses ABA-inducible VPR-Sa dCas9DepositorInsertsABI-tagBFP-Sa dCas9
PYL1-VPR
UseCRISPR and Synthetic Biology; PiggybacTagsABI-tagBFP-Nucleoplasmin NLS, IRES-Zeocin, PYL1, …ExpressionMammalianMutationD10A, N580A and Synonymous mutation in PYL1 to re…PromoterCAG and PGKAvailable sinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDonor-GDH
Plasmid#140629PurposeEncodes a mini-transposon derived from V. cholerae Tn6677 and T7-GDH cargo gene.DepositorInsertMini-Tn6677, T7lac-GDH gene cassette
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-2A-rtTA-TcVA
Plasmid#24592DepositorInsertGateway(TM) cassette
UseLentiviralTagsVAExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2010AvailabilityAcademic Institutions and Nonprofits only