Addgene

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pCellFree_G03 RAP2A

Plasmid

#67058

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

RAP2A (RAP2A Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
Lifeact-rsLOV1 pcDNA3.1(+)

Plasmid

#107881

Purpose

Mammalian expression of Lifeact-rsLOV1

Depositor

Stefan Hell

Article

Gregor et al Sci Rep. 2018 Feb 9;8(1):2724. doi: 10.1

Insert

rsLOV1

Use

Tags

Lifeact

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0007

Plasmid

#117534

Purpose

Level0 Golden Gate part: CDS, LbCas12a

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

LbCas12a (with stop codon)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pTRANS_202

Plasmid

#91106

Purpose

Transformation, Type: T-DNA, Plant Selection: none, Viral Replication: ToLCV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

No Promoter

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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PGRP-SA

Plasmid

#21055

Depositor

Johann Deisenhofer

Article

Chang et al PLoS Biol. 2004 Sep . 2(9):E277.

Insert

PGRP-SA (PGRP-SA Fly)

Use

Tags

His

Expression

Insect

Mutation

Promoter

Available since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pJZC548

Plasmid

#62316

Purpose

sgRNA with 1x PP7 for yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA + 1x PP7

Use

Tags

Expression

Yeast

Mutation

Promoter

SNR52

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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TAL3030

Plasmid

#41204

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-bbc3-(puma)-Left (bbc3 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
pc3XB-ZF5

Plasmid

#13104

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF5

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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PLEXIN A3

Plasmid

#58238

Purpose

Riboprobe for in vitro transcription of Plexin A3

Depositor

Christiana Ruhrberg

Article

Schwarz et al Dev Biol. 2008 Dec 1;324(1):1-9. doi: 10

Insert

Plexin A3 (Plxna3 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
pBDKRB2_TCS(Q’L)_HA-dCas9(N)_DHFR

Plasmid

#101109

Purpose

Encodes dCas9(N)-DHFR fused to BDKRB2

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

BDKRB2-dCas9(N)-DHFR

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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dSp-VP64-p65-RTA(75-190)

Plasmid

#99670

Purpose

Expresses dSp Cas9 fused to combination of truncated activation domains

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

VP64-p65-RTA(75-190)

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

May 30, 2020

Availability

Academic Institutions and Nonprofits only

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pJK_proK6_eGFP

Plasmid

#59850

Purpose

Constitutively expresses eGFP with proK6 promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

Enhanced Green Fluorescent Protein

Use

Tags

6x-His

Expression

Bacterial

Mutation

S2R,S65G,S72A

Promoter

proK6

Available since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pBW112pBAD-hrpV

Plasmid

#78639

Purpose

pSB3K3 carrying araC-PBAD

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

araC-PBAD

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

see insert

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pAWp7-10

Plasmid

#72742

Purpose

pFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124]

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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CD4_LD 0_TEV

Plasmid

#58870

Purpose

MESA protease chain with CD4 ectodomain and no linker domain

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA protease chain with CD4 ectodomain and TEV protease

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pC0.122

Plasmid

#119629

Purpose

Level 0 Part. CDS

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

sgRNA scaffold

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.203

Plasmid

#119604

Purpose

Level 0 part. TSS promoter

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

J23103_TSS

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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TAL3144

Plasmid

#41318

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-pum1-Left (pum1 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
TAL3145

Plasmid

#41319

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-pum1-Right (pum1 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
TAL3200

Plasmid

#41374

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-vascular-endothelia-growth-factor-aa-(vegfaa)-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2012

Availability

Academic Institutions and Nonprofits only