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Plasmid#139505PurposeAAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.DepositorHas ServiceAAV8 and AAV9Insertparapinopsin
UseAAVTagsVenus tag following 3x Ala linkerExpressionMammalianMutationPromoterEf1aAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1 KSR2
Plasmid#25968DepositorInsertKinase suppressor of Ras 2 (Ksr2 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMB1_1R26PylRS(CbzK)_AfTyrRS(p-I-Phe)_AlvtRNA-ΔNPyl(8)(CGA)_AftRNA-Tyr(A01)(CUA)
Plasmid#174516PurposeRecoded double aaRS/tRNA plasmid with 1R26PylRS/AlvtRNA-ΔNPyl(8)(CGA) & AfTyrRS/AftRNA-Tyr(A01)(CUA) to incorporate CbzK into the TCG codon & p-I-Phe into the TAG codon.DepositorInsertsPylS_1R26 - CBZK
Af_TyrRS - p-I-Phe
Ma tRNA pylT(8) with CGA anticodon
A.fulgidus tRNA with CUA anticodon
UseTagsExpressionBacterialMutationCUA anticodon, M.alvus Pyl tRNA with CGA anticodo…PromoterGlnS and lppAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6-sgRNA(OXTR.1)-CMV-eGFP
Plasmid#194015PurposeExpresses a gRNA that targets OXTR coding sequence in multiple (rodent) species and eGFPDepositorInsertssgRNA(Oxtr.1)
eGFP
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterCMV and u6Available sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag TK1
Plasmid#20652DepositorInsertTK1 (TK1 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag PLAU
Plasmid#20588DepositorInsertPLAU (PLAU Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pT3-EFa1-HA-Jag1
Plasmid#46051DepositorInsertJag1 (Jag1 Rat)
UseTagsHA tagExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag PDK1
Plasmid#20564DepositorInsertPDK1 (PDK1 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti NLuc398-G8NCRD IRES G8NCRD-CLuc394
Plasmid#128386PurposeExpresses a split firefly luciferase reporter based on the N terminal carbohydrate recognition domain of human galectin 8, which concentrates inside endosomes following endosomal disruptionDepositorUseLentiviral, Luciferase, and Synthetic BiologyTagsC-terminal luc2 fragment and N-terminal luc2 frag…ExpressionMammalianMutationN terminal carbohydrate recognition domain fused …PromoterEF1A and IRESAvailable sinceJan. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag SGK
Plasmid#20628DepositorInsertSGK (SGK1 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag PRKCD
Plasmid#20603DepositorInsertPRKCD (PRKCD Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Neo-Myr-Flag-IKBKE
Plasmid#15267DepositorInsertIKBKE (IKBKE Human)
UseRetroviralTagsMyr-FlagExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTBL1269 pLenti-CHYRON17-pEF1a(short)-Luc2-P2A-tdTomato-T2A-TdT
Plasmid#163643PurposeMakes a lentivirus that expresses hgRNA with 17nt spacer and Luc2, tdTomato, and human TdT.DepositorInsertsUseLentiviralTagsLuc2-P2A-tdTomato-T2AExpressionMutationThe SpCas9 sgRNA constant region is mutated to ma…PromoterEFS and pU6Available sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag PLK1
Plasmid#20589DepositorInsertPLK1 (PLK1 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-H-RAS V12
Plasmid#31201DepositorInsertH-Ras (HRAS Human)
UseGateway donor vectorTagsExpressionMutationG12V constitutively activePromoterAvailable sinceJuly 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2CG2-mnx1:mCherry-PA-Rac1
Plasmid#169044PurposeDestination transgenesis vector for expressing mCherry fused with an optimized photoactivatable Rac1 protein in zebrafish spinal motor neurons using 3 tandem repeats of the mnx1 enhancer.DepositorInsertPA-Rac-1 (RAC1 Avena sativa (oat), Human)
UseTags6X His and Fused with mCherryExpressionMammalianMutationRac1 starts at I4 and contains mutations Q61L, E9…Promoter3xMnx1Available sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag MOBKL2A
Plasmid#20633DepositorInsertMOBKL2A (MOBKL2A Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag PIP5K1A
Plasmid#20580DepositorInsertPIP5K1A (PIP5K1A Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag PIK3CB
Plasmid#20573DepositorInsertPIK3CB (PIK3CB Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
R620-M13-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 B.1.1.7
Plasmid#170197PurposeMammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.1.7 variant)DepositorInsertSARS-CoV-2 S (spike) B.1.1.7 (S SARS-CoV-2)
UseTagsHis6ExpressionMammalianMutationΔ(69-70), Δ144, N501Y, A570D, D614G, P681H, T716I…PromoterCMV51Available sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only