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CamKII-LGI1-pHluorin

Plasmid

#185537

Purpose

Expresses LGI1-pHluorin under the CamKII promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

LGI1 (Lgi1 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mammalian

Mutation

Promoter

CamKII

Available since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-EGFP-CNA35

Plasmid

#61603

Purpose

bacterial expression of N-terminal fusion protein of CNA35 with EGFP

Depositor

Maarten Merkx

Article

Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

Insert

EGFP and CNA35

Use

Tags

His tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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PB-REVERBA-mScarlet-NLS-PEST

Plasmid

#190658

Purpose

Fluorescent reporter for circadian rhythms

Depositor

Mary Teruel

Article

Zhang et al Proc Natl Acad Sci U S A. 2022 Aug 16;11

Insert

Reverba-mScarlet (Nr1d1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3-cMYC promoter

Plasmid

#114724

Purpose

reporter luciferase construct

Depositor

Martine Roussel

Article

Roussel et al Oncogene. 1994 Feb;9(2):405-15.

Insert

C-Myc promoter region

Use

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-iRFP670-CaaX

Plasmid

#170464

Purpose

Expresses iRFP670 fluorophore targeted to the plasma membrane using a prenylation motif

Depositor

John James

Article

Harris et al Mol Syst Biol. 2021 May;17(5):e10091. do

Insert

iRFP670-CaaX

Use

Lentiviral

Tags

CaaX

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pFH2.2_mCherry-MS2x12

Plasmid

#205521

Purpose

Express mCherry-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mCherry

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pmAmetrine-DEVD-tdTomato

Plasmid

#18879

Purpose

Caspase-3 FRET biosensor, mammalian expression of mAmetrine-DEVD-tdTomato

Depositor

Robert Campbell

Article

Ai et al Nat Methods. 2008 May . 5(5):401-3.

Insert

mAmetrine-DEVD-tdTomato (CASP3 Human, lab constructed)

Use

Tags

Expression

Mammalian

Mutation

mAmetrine-tdTomato linker sequence: ...ITLGGTGSGS…

Promoter

Available since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only

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UbC-(mNeonGreen)4-tDeg

Plasmid

#129404

Purpose

(mNeonGreen)4-tDeg fluorogenic protein

Depositor

Samie Jaffrey

Article

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Insert

(mNeonGreen)4-tDeg

Use

Tags

Expression

Mammalian

Mutation

Promoter

UbC

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pET19-TdTwt

Plasmid

#207466

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

Depositor

Jay Keasling

Article

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

TdTwt (Dntt Mouse)

Use

Tags

10xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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G protein alpha-s(alpha3beta5/G226A/A366S)

Plasmid

#55797

Purpose

This highly effective dominant negative G protein alpha-s mutant contains, in addition to the mutations in alpha-s(alpha3beta5/G226A), the A366S mutation, which increases GDP release.

Depositor

Catherine Berlot

Article

Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.

Insert

alpha-s (alpha3beta5/G226A/A366S) (Gnas Rat)

Use

Tags

internal EE epitope (residues 189-194 in alpha-s …

Expression

Mammalian

Mutation

N271K, K274D, R280K, T284D, I285T, G226A, A366S i…

Promoter

CMV

Available since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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