Skip to main content
Addgene
Showing: 9201 - 9220 of 11049 results

  4. pET-28a(+)::NL

    Plasmid
    #141289
    Purpose
    NLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)
    Depositor
    Andrew Millar
    Article
    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10
    Insert
    NLUC
    Use
    Luciferase
    Tags
    Expression
    Bacterial
    Mutation
    Engineered for high stability (t1/2ā€‰=ā€‰11.5 days aā€¦
    Promoter
    promoter for bacteriophage T7 RNA polymerase
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    141289-plasmid-map-sequence-id-324904

    Enlarge

  10. SpyTag-Ī²-Lactamase-SpyCatcher

    Plasmid
    #52656
    Purpose
    Expresses SpyTag-SpyCatcher mediated cyclized version of TEM-1 Ī²-lactamase (BLA). Cyclization of BLA led to an increase in thermal resilience of >60 degrees celsius.
    Depositor
    Mark Howarth
    Article
    Schoene et al Angew Chem Int Ed Engl. 2014 Jun 10;53(2
    Insert
    TEM-1 Ī²-Lactamase
    Use
    Tags
    SpyCatcher and SpyTag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    May 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    52656-plasmid-map-sequence-id-422741

    Enlarge

  15. pCiPS-NIFV

    Plasmid
    #110807
    Purpose
    Non-Integrating Foamy Virus pol-expressing helper plasmid with mutations in the DDE catalyic core motif of the integrase sequence, remaining as unintegated episomes in transduced cells
    Depositor
    David Russell
    Article
    Deyle et al J Virol. 2010 Sep;84(18):9341-9. doi: 10
    Insert
    Pol
    Use
    Tags
    Expression
    Mammalian
    Mutation
    2 mutations in the DDE catalyic core motif of theā€¦
    Promoter
    CMV
    Available since
    June 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    110807-plasmid-map-sequence-id-222708

    Enlarge

  17. pCSIIhyg-rtTA

    Plasmid
    #139480
    Purpose
    Expresses rtTA, Tet-ON reverse tetracycline-controlled trans-activator in mammalian cells.
    Depositor
    Kazuhiro Aoki
    Article
    Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2
    Insert
    rtTA, Tet-ON reverse transactivator
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human EF1a promoter
    Available since
    March 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    139480-plasmid-map-sequence-id-276014

    Enlarge

Showing: 9201 - 9220 of 11049 results