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Plasmid#175595PurposeTet inducible, KAN Resistant, non labelled synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.DepositorInsertCatalytic domain of E.coli relA gene.
UseTagsExpressionBacterialMutationOnly the catalytic domain of the enzyme is used (…PromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG_mRuby2_smFP OLLAS
Plasmid#59761Purposeencodes multiple copies of an epitope tag OLLAS ( not detected by anti GFP ab)DepositorInsertmRuby2_smFP_OLLAS
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pH2B-emiRFP703
Plasmid#136567PurposeHistone 2B labeling with near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)DepositorInsertH2B-emiRFP703
UseTagsemiRFP703ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SlugCas9-NNG-P2A-puro
Plasmid#214352PurposeExpresses SlugCas9-NNG and puromycin resistance genesDepositorInsertSlugCas9-NNG and puromycin resistance genes
UseTagsExpressionMammalianMutationQ782R/S888R/L906R/N984S/E1012K/K1016IPromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG-OTUB1 C91S-pcDNA3.1
Plasmid#118210PurposeExpresses FLAG-OTUB1 C91S catalytic inactive mutant in mammalian cellsDepositorInsertOTUB1 (OTUB1 Human)
UseTags3x FLAGExpressionMammalianMutationchanged cysteine 91 to serinePromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pK7WGF2::hCas9
Plasmid#46965PurposeExpesses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissueDepositorInserthCas9
UseCRISPR; Plant expressionTagseGFPExpressionMutationPromoter35SAvailable sinceAug. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-OgNLuc
Plasmid#208851PurposeExpresses FLAG epitope-tagged OgNLuc "Orange" BRET reporter in mammalian cells.DepositorInsertOgNLuc LumiFluor
UseLentiviralTags1xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
SaCas9v2-Puro
Plasmid#178802PurposeSaCas9 with 2A-Puro, and a cloning backbone for sgRNA.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterCbh (Cas9-2A Puro) and U6 (gRNA)Available sinceAug. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
mEos2-Integrin-Beta1-N-18
Plasmid#57390PurposeLocalization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertIntegrin-Beta1 (ITGB1 Human)
UseTagsmEos2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-CMV-CD4-sTC-M13-GFP-IRES-CaM-V5-sTN
Plasmid#219782PurposeCaST-IRES for HEK cell expressionDepositorInsertCD4-sTC-M13-GFP-IRES-CaM-V5-sTN
UseTagsHA and V5ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
mEos2-Integrin-Alpha2-N-18
Plasmid#57389PurposeLocalization: Integrin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertIntegrin-Alpha2 (ITGA2 Human)
UseTagsmEos2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA-DEST53 PINK1 N-GFP
Plasmid#13315DepositorInsertPINK1 (PINK1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pWZL Blast DN E-cadherin
Plasmid#18800DepositorInsertDom neg E-cadherin (Cdh1 Mouse)
UseRetroviralTagsExpressionMammalianMutationtruncated form of E-cadherin (dominant negative) …PromoterAvailable sinceJuly 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 mSynCAM3
Plasmid#115498Purposeexpression of mouse full-length SynCAM 3DepositorInsertCadm3 (Cadm3 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Ruby2sm-Flag.WPRE.SV40
Plasmid#98925PurposeAAV expression of Ruby2 spaghetti monster FLAG constructDepositorInsertRuby2-smFP-Flag
UseAAVTagsExpressionMammalianMutationPromoterCBAAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
Pveg
Plasmid#55173PurposeVery strong constitutive promoterDepositorInsertveg promoter
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromotervegAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBAD/His-B-iRFP
Plasmid#31855Purposea phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imagingDepositorInsertiRFP
UseTagsExpressionBacterialMutationS13L, A92T, V104I, V114I, E161K, Y193K, F198Y, D2…PromoteraraBADAvailable sinceSept. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-SauriCas9
Plasmid#135964PurposeExpresses SauriCas9, and cloning backbone for sgRNADepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-mTagBFP2-MRTF-A
Plasmid#182061PurposeMammalian expression vector encoding myocardin-related transcription factor A tagged with mTagBFP2.DepositorInsertmTagBFP2-MRTF-A (MRTFA Human)
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiX2-PURO-shLKB1-Ms
Plasmid#61231PurposeLentivirus expressing shRNA to mouse LKB1DepositorInsertshLkb1 (Ms/Hu) (Stk11 Mouse, Human)
UseLentiviralTagsExpressionMutationPromoterH1/TOAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only