Skip to main content
Addgene
Showing: 9181 - 9200 of 72327 results

  2. L4683

    Plasmid
    #1672
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    myo3-2341, myo3Tsa, gfpN, IVSR, gfpGLA, IVSS, IVS…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1672-plasmid-map-sequence-id-282

    Enlarge

  3. pcDNA3.1(-)-qmTQ2-cAMP

    Plasmid
    #225250
    Purpose
    mammalian expression of qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMP
    Depositor
    Yasushi Okada
    Insert
    qmTQ2-cAMP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    225250-plasmid-map-sequence-id-446184

    Enlarge

  4. GPD1_SB

    Plasmid
    #224147
    Purpose
    IPTG-inducible expression of C. elegans protein GPD-1 with a chitin tag
    Depositor
    Katherine Walstrom
    Insert
    GPD-1 (gpd-1 Nematode)
    Use
    Tags
    Ala-intein-CBD
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    224147-plasmid-map-sequence-id-444366

    Enlarge

  5. ADH1_KW

    Plasmid
    #224149
    Purpose
    IPTG-inducible expression of C. elegans protein ADH-1 with a chitin tag
    Depositor
    Katherine Walstrom
    Insert
    ADH-1 (sodh-1 Nematode)
    Use
    Tags
    Ala-intein-CBD
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    224149-plasmid-map-sequence-id-444365

    Enlarge

  6. GPD3_KW

    Plasmid
    #224151
    Purpose
    IPTG-inducible expression of C. elegans protein GPD-3 with a chitin tag
    Depositor
    Katherine Walstrom
    Insert
    GPD-3 (gpd-3 Nematode)
    Use
    Tags
    intein-CBD
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    224151-plasmid-map-sequence-id-444362

    Enlarge

  7. pT7-NSP3SA11-P2A-NSP5(wt)-SNAP

    Plasmid
    #220809
    Purpose
    Express separately the Rotavirus SA11 NSP3 protein and the Rotavirus SA11 NSP5 fused with SNAP-tag
    Depositor
    Alex Borodavka
    Insert
    Rotavirus SA11 NSP3-P2A-NSP5(wt)-SNAP
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    220809-plasmid-map-sequence-id-443929

    Enlarge

  8. pT7-NSP1delta103CSA11-P2A-GFP

    Plasmid
    #220814
    Purpose
    Express separately the Rotavirus SA11 delta103C NSP1 protein and the sfGFP (Pedelacq et al., 2006)
    Depositor
    Alex Borodavka
    Insert
    Rotavirus SA11 NSP1delta103C-P2A-GFP
    Use
    Other
    Tags
    Expression
    Mutation
    Lacks C-terminal 103 aa
    Promoter
    Available since
    Aug. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    220814-plasmid-map-sequence-id-443928

    Enlarge

  9. pDonorTp-fugfp

    Plasmid
    #211790
    Purpose
    pDonorTp with addition of fugfp gene from pUS252 outside of the FRT sites
    Depositor
    Silvia Cardona
    Insert
    fuGFP
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    211790-plasmid-map-sequence-id-443284

    Enlarge

  10. pFastBac HTB His-TEV-rDPP9-FLAG

    Plasmid
    #217328
    Purpose
    Purification of rat DPP9 fusion with C-terminal FLAG tag from insect cells
    Depositor
    Yifan Wang
    Insert
    DPP9
    Use
    Tags
    FLAG and His-TEV
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217328-plasmid-map-sequence-id-433437

    Enlarge

  11. pFastBac HTB His-TEV-rDPP9(S729A)

    Plasmid
    #217327
    Purpose
    Purification of catalytically-dead rat DPP9 from insect cells
    Depositor
    Yifan Wang
    Insert
    DPP9
    Use
    Tags
    His-TEV
    Expression
    Insect
    Mutation
    S729A
    Promoter
    polyhedrin
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217327-plasmid-map-sequence-id-433438

    Enlarge

  12. pcDNA-DDX53-FLAG

    Plasmid
    #222241
    Purpose
    Express FLAG tagged DDX53 in mammalian cells
    Depositor
    Yuliang Wu
    Insert
    DDX53 (DDX53 Human)
    Use
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    T7
    Available since
    Aug. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    222241-plasmid-map-sequence-id-441350

    Enlarge

  13. pET29a-DDX53-Q249A

    Plasmid
    #222216
    Purpose
    Express DDX53 mutant Q249A protein in bacteria
    Depositor
    Yuliang Wu
    Insert
    DDX53_Q249A (DDX53 Human)
    Use
    Tags
    His Tag
    Expression
    Bacterial
    Mutation
    K272A
    Promoter
    T7
    Available since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    222216-plasmid-map-sequence-id-441427

    Enlarge

  14. pET29a-DDX53-K272A

    Plasmid
    #222215
    Purpose
    Express DDX53 mutant K272A protein in bacteria
    Depositor
    Yuliang Wu
    Insert
    DDX53_K272A (DDX53 Human)
    Use
    Tags
    His Tag
    Expression
    Bacterial
    Mutation
    K272A
    Promoter
    T7
    Available since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    222215-plasmid-map-sequence-id-441662

    Enlarge

  15. pET29a-DDX53-E377Q

    Plasmid
    #222217
    Purpose
    Express DDX53 mutant E377Q protein in bacteria
    Depositor
    Yuliang Wu
    Insert
    DDX53_E377Q (DDX53 Human)
    Use
    Tags
    His Tag
    Expression
    Bacterial
    Mutation
    E377Q
    Promoter
    T7
    Available since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    222217-plasmid-map-sequence-id-441653

    Enlarge

  16. pcDNA-DDX53-K272A

    Plasmid
    #222242
    Purpose
    Express FLAG tagged DDX53 mutant K272A in mammalian cells
    Depositor
    Yuliang Wu
    Insert
    DDX53 (DDX53 Human)
    Use
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    K272A
    Promoter
    T7
    Available since
    Aug. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    222242-plasmid-map-sequence-id-441373

    Enlarge

  17. pUC57_beta-globin-5'UTR_Nanoluc

    Plasmid
    #219989
    Purpose
    betaglobin-5'UTR-NanoLuc mRNA synthesis
    Depositor
    Simon Alberti
    Insert
    betaglobin-5'UTR-NanoLuc (HBB Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219989-plasmid-map-sequence-id-436835

    Enlarge

  18. pOCC102-MBP-eIF4G-SkA

    Plasmid
    #219977
    Purpose
    eIF4G-SkA protein purification
    Depositor
    Simon Alberti
    Insert
    eIF4G-SkA (TIF4631 Budding Yeast)
    Use
    Tags
    6xHis-MBP
    Expression
    Insect
    Mutation
    eIF4A binding domain of S. kudriavzevii
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219977-plasmid-map-sequence-id-436800

    Enlarge

  19. pOCC102-MBP-eIF4G-RAR

    Plasmid
    #219973
    Purpose
    eIF4G-RAR protein purification
    Depositor
    Simon Alberti
    Insert
    eIF4G-RAR (TIF4631 Budding Yeast)
    Use
    Tags
    6xHis-MBP
    Expression
    Insect
    Mutation
    Truncation: amino acids 490 to 942
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219973-plasmid-map-sequence-id-436750

    Enlarge

  20. His-GST-MPK6

    Plasmid
    #215170
    Purpose
    Encodes the glutathionine S-transferase with N-terminal His tag and fused to the N-terminus of a mitogen-activated protein kinase 6 from Arabidopsis thaliana
    Depositor
    Travis Beddoe, Bhuvana Shanbhag
    Insert
    His-GST-MPK6
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac
    Available since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    215170-plasmid-map-sequence-id-430166

    Enlarge

Showing: 9181 - 9200 of 72327 results