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Plasmid#183536PurposeOptogeneticsDepositorInsertChRmine-oScarlet-Kv2.1
UseAAVTagsExpressionMutationNonePromoterCaMKIIaAvailable sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Puro-CAG-ASAP2s
Plasmid#101274PurposeGenetically encoded voltage indicator ASAP2s expressed under strong mammalian promoter (CAG)DepositorInsertASAP2s
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV Syn 2xLyn-ERex-Venus-bPAC(F198Y)
Plasmid#165491PurposeNeuronal expression of PACmn, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity .DepositorInsert2xLyn-ERex-Venus-bPAC(F198Y)
UseAAVTagsVenusExpressionMammalianMutationPromoterhSyn1Available sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A mKate2-hu_ncOGT D554N
Plasmid#154283PurposeEntry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; D554N variant, no Ser/Thr glycosylation)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseGateway entry vectorTags2x FLAG and mKate2ExpressionMutationD554N mutation (Ser/Thr Glycosylation Impaired)PromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A mKate2-hu_ncOGT 5N5A
Plasmid#154285PurposeEntry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; 5N5A variant, no HCF-1 cleavage)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseGateway entry vectorTags2x FLAG and mKate2ExpressionMutationN322A, N356A, N390A, N424A, N458A mutations (HCF-…PromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCCLc-MND-B2705-SABR-Backbone
Plasmid#119051Purpose3rd gen transfer vector. HLA-B2705 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains a stuffer fragment flanked by BsmBI sites at the epitope cloning site.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
ePB-PURO-FLAG-METTL3-APPA
Plasmid#160253PurposeFor stable integration of catalytic inactive FLAG-METTL3 in human cells with trasposaseDepositorInsertmethyltransferase like 3 (METTL3 Human)
UseTagsFLAGExpressionMammalianMutationchanges in aa 395–398, DPPW → APPAPromotertight TRE promoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-N90-beta-catenin
Plasmid#31787DepositorInsertbeta-catenin (CTNNB1 Human)
UseEntry vectorTagsMycExpressionMutationDeletion of aa 1-90- Constitutively ActivePromoterAvailable sinceOct. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hPPP3CA-FLAG
Plasmid#179134PurposeExpression vector of human protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.DepositorInsertprotein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-EglN2-pcDNA3
Plasmid#18961DepositorInsertEgg laying Nine - 2 (EGLN2 Human)
UseTagsHA-tagExpressionMammalianMutationwt cDNAPromoterAvailable sinceJune 19, 2009AvailabilityAcademic Institutions and Nonprofits only -
pUC19-E3CY56
Plasmid#103075PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertE3CY56(Cas9 coding gene from Aminomonas paucivorans DSM 12260)
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSW683 - pAHP6>>
Plasmid#115998Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpAHP6:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HtrA2-FLAG
Plasmid#15938DepositorInsertHtrA2 (HTRA2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-cpPKN-mRuby2-GFP1-10
Plasmid#181859PurposePart of FluoSTEP-RhoA biosensor; must be expressed with GFP11-tagged RhoA plasmid (Addgene #181860) or in GFP11-RhoA gene-edited cells.DepositorInsertcp-PKN-mRuby2-GFP(1-0)
UseTagsmRuby2 and sfGFP(1-10)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_YWHAZ_14-3-3
Plasmid#109808PurposeProtein expression and purification of YWHAZ_14-3-3DepositorInsertYWHAZ_14-3-3 (YWHAZ Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6sgp53-U6sgbbSapI-GFAPCre
Plasmid#100275PurposeAAV-CRISPR mediated genome editing in glia / astrocytesDepositorTypeEmpty backboneUseAAV, CRISPR, Mouse Targeting, and Synthetic Biolo…TagsExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-IRF5
Plasmid#178450Purposedoxycycline-inducible expression of Human IRF5 in mammalian cellsDepositorInsertIRF5 (IRF5 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Mito-AcGFP-MTn
Plasmid#154350PurposeMammalian expression of MTnDepositorInsertMTn
UseTagsAcGFPExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hs-CDC34
Plasmid#52731Purposeretroviral expression of human CDC34DepositorInsertCDC34 (CDC34 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro-tetON-shGFP
Plasmid#110939PurposeTet-inducible shRNA targeting GFPDepositorInsertshGFP
UseLentiviral and RNAiTagsExpressionMutationPromoterH1/TOAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only