Addgene

Showing: 4591 - 4600 of 147948 results

Results per page:

Sort results by:

pcDNA3-FLAG-Luciferase

Plasmid

#58792

Purpose

Express FLAG-tagged Luciferase in mammalian cells

Depositor

Hiroyoshi Ariga

Article

Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

Insert

firefly Luciferase

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2014

Availability

Academic Institutions and Nonprofits only
pJRH051

Plasmid

#171625

Purpose

2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreen

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-BsmBI-Destination
mNeon

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1-a and U6

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
FUGW-Cytosol-TurboID

Plasmid

#200961

Purpose

3rd gen lentiviral plasmid for expressing cytosol localized TurboID with EGFP

Depositor

Keriann Backus

Article

Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

Insert

Cytosol-TurboID

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
BC1209-pHR-dSV40-dCas9-14xGFP11-P2A-BFP

Plasmid

#164038

Purpose

Expression of dCas9-14xGFP11 in mammalian cells

Depositor

Baohui Chen

Article

Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

Insert

dCas9-14xGFP11

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A and H840A in Cas9

Promoter

dSV40

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-calpainsensor

Plasmid

#36182

Purpose

detection of activity of ubiquitous calpains

Depositor

Isabelle Richard

Article

Stockholm et al J Mol Biol. 2005 Feb 11;346(1):215-22. E

Insert

alpha-Fodrin cleavage site (Sptan1 Mouse)

Use

Tags

eCFP and eYFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pKNOCK-Km

Plasmid

#46262

Purpose

Bacterial targeting

Depositor

Mikhail Alexeyev

Article

Alexeyev et al Biotechniques. 1999 May;26(5):824-6, 828

Type

Empty backbone

Use

Bacterial targeting

Tags

Expression

Mutation

Promoter

Available since

Aug. 14, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pSMPP-mCherry-hTRIM21

Plasmid

#104972

Purpose

Lentiviral Transfer Plasmid with mCherry-tagged human TRIM21

Depositor

Leo James

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

Tripartite Motif containing 21 (TRIM21 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mutation

Promoter

SFFV

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pArk313

Plasmid

#200257

Purpose

Ark313 rep/cap

Depositor

Justin Eyquem

Article

Nyberg et al Cell. 2023 Jan 19;186(2):446-460.e19. do

Insert

Ark313 Rep/Cap

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRT_TO_FlagHA_ELAVL1

Plasmid

#106104

Purpose

Inducible gene expression of FlagHA ELAVL1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

ELAVL1 (ELAVL1 Human)

Use

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
NKP38 GFP (1009)

Plasmid

#62021

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Nucleus. 2012 Nov-Dec;3(6):552-64. doi:

Insert

AYTL1 (LPCAT2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only