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Plasmid#107738PurposeCan be used to express Cre recombinase and simultaneously the red fluorescent protein dTomato. Can also be used to create adeno-associated virus for delivery of these genes.DepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertCre, dTomato
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterhSynAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
XLone-BSD SARS-CoV2 N P2A mCherry
Plasmid#154398PurposePiggybacTransposon-based tunable and temporal expression control of SARS-CoV2 N and mCherryDepositorInsertSARS-CoV2 N Protein (N SARS-CoV2)
UsePiggybacTagsmCherryExpressionMammalianMutationPromoterTRE3GAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SCP1-dSa VPR mini.-2X snRP-1 BsaI gRNA
Plasmid#99698PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA with BsaI cloning sites for programming, vector allows for strong activation of programmed target gene, can be packaged and delivered as AAVDepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterAvailable sinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-6His-SUMO-Nsp10
Plasmid#154959PurposeExpresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coliDepositorInsert6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
UseTags6His-Thrombin-SUMOExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28-6His-TEV-Nsp14
Plasmid#154954PurposeExpresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable 6His tag in E. coliDepositorInsert6His-TEV-Nsp14 (ORF1ab SARS-CoV-2)
UseTags6His-TEVExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJAT74
Plasmid#204296PurposepJAT plasmid with multiple cloning site for introducing custom payload. One U6 promoter.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgDrd2
Plasmid#159918PurposeMutagenesis of Drd2DepositorInsertDrd2 (Drd2 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZD-CJ1111
Plasmid#175058PurposerbcLXS6803 integrative plasmid expresses CRE recombinase as a translational fusion to rbcL6803DepositorInsertCRE
UseUnspecifiedTagsExpressionMutationPromoterrbcLXSAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgSlc32a1
Plasmid#159905PurposeMutagenesis of Slc32a1DepositorInsertSlc32a1 (Slc32a1 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJUNB.1.0-gDNA
Plasmid#112409PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor JUNBDepositorInsertJUNB (JUNB Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s
Plasmid#105715PurposeIn the presence of Cre, the plasmid can be used to express GCaMP6s fused to the presynaptic protein synaptophysin. Drives enriched expression of GCaMP6s in neuronal axon boutons.DepositorHas ServiceAAV5Insertsynaptophysin-GCaMP6s
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEf1a, Human elongation factor-1 alphaAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
ANO10 (TMEM16KA-c001)
Plasmid#110267PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertTMEM16K (ANO10 Human)
UseTagsTEV-His10-FlagExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pATF4.1.0-gDNA
Plasmid#113772PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ATF4DepositorInsertATF4 (ATF4 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgDrd1
Plasmid#159917PurposeMutagenesis of Drd1DepositorInsertDrd1 (Drd1 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
JMJD1CA-c089
Plasmid#79644PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertJMJD1C (JMJD1C Human)
UseTags6x His and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pICE ADE2
Plasmid#122876PurposeADE2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceMay 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET22-Nsp10-TEV-6His
Plasmid#154957PurposeExpresses Nsp10 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coliDepositorInsertNsp10-TEV-6His (ORF1ab SARS-CoV-2)
UseTagsTEV-6HisExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJuly 21, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pICE URA3
Plasmid#122882PurposeURA3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceDec. 3, 2019AvailabilityAcademic Institutions and Nonprofits only