Addgene

We narrowed to 1,351 results for: pUC57

Showing: 901 - 920 of 1351 results

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pRExT2A-NT-PmNG

Plasmid

#232200

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy-mNeonGreen

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

mNeongreen

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pRExT2A-CT-BmNG

Plasmid

#232207

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mNeongreen-3xTy-T2A-BSD-3xTy

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

mNeongreen

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pRExT2A-CT-BTy

Plasmid

#232209

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: 3xTy-T2A-BSD-3xTy

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

3xTy

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pRExT2A-NT-PTy

Plasmid

#232202

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

3xTy

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRExT2A-NT-PmSc

Plasmid

#232548

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy-mScarlet

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

mScarlet

Use

Tags

Expression

Bacterial

Mutation

None

Promoter

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRExT2A-CT-PmNG

Plasmid

#232201

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mNeongreen-3xTy-T2A-PAC-3xTy

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

mNeongreen

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pT7-AGG-NLS-EGFP

Plasmid

#214602

Purpose

express EGFP ; in vitro transcribed modified RNA vector

Depositor

Yuxuan Guo

Article

Huang et al Int Immunopharmacol. 2024 Oct 17;143(Pt

Insert

EGFP

Use

Unspecified

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-AGG-SpCas9

Plasmid

#214603

Purpose

encode SpCas9; in vitro transcribed modified RNA vector

Depositor

Yuxuan Guo

Article

Huang et al Int Immunopharmacol. 2024 Oct 17;143(Pt

Insert

SpCas9

Use

CRISPR and Unspecified

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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AA019

Plasmid

#216039

Purpose

Fragmid fragment: (C' terminus) VP64; NpNLS

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

VP64_v2.1; NpNLS_v1.1

Use

Fragment

Tags

Expression

Mutation

Promoter

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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AA294

Plasmid

#215968

Purpose

Fragmid fragment: (Pol 2 promoter) U1 small nuclear RNA promoter

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U1a_v1

Use

Fragment

Tags

Expression

Mutation

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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AA018

Plasmid

#215976

Purpose

Fragmid fragment: (N' terminus) 4xVP16 transcritional activators

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; BPNLS_v1.1; VP64_v1.1

Use

Fragment

Tags

Expression

Mutation

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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AA177

Plasmid

#215983

Purpose

Fragmid fragment: (N' terminus) Start codon only

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start

Use

Fragment

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AA134

Plasmid

#216044

Purpose

Fragmid fragment: (C' terminus) SV40NLS; Linker; MeCP2

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

SV40NLS_v1.7; Linker_v4.1; MeCP2_v1.1

Use

Fragment

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA006

Plasmid

#216056

Purpose

Fragmid fragment: (2A Selection) Blasticidin resistance

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

T2A_v1.1; BlastR_v1.1; Stop

Use

Fragment

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA199

Plasmid

#216050

Purpose

Fragmid fragment: (C' terminus) p65; NpNLS

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

p65_v1.1; NpNLS_v1.1

Use

Fragment

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AA266

Plasmid

#216064

Purpose

Fragmid fragment: (2A Selection) TagRFP

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

P2A_v1.1; TagRFP_v1.1; Stop

Use

Fragment

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA267

Plasmid

#216065

Purpose

Fragmid fragment: (2A Selection) TagRFP657

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

P2A_v1.1; TagRFP657_v1.1; Stop

Use

Fragment

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AA200

Plasmid

#216051

Purpose

Fragmid fragment: (C' terminus) p65; HSF1; NpNLS

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

p65_v1.2; HSF1_v1.1; NpNLS_v1.1

Use

Fragment

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AA414

Plasmid

#215991

Purpose

Fragmid fragment: (N' terminus) conditional degradation

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; V5_v1.1; HaloTag_v1.1

Use

Fragment

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA093

Plasmid

#216042

Purpose

Fragmid fragment: (C' terminus) ZIM3 KRAB

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

NpNLS_v1.1; KRAB_v3.1

Use

Fragment

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge