Addgene

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pCI-SEP_NR2B

Plasmid

#23998

Depositor

Robert Malinow

Article

Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

Insert

NR2B (Grin2b Rat)

Use

Tags

NR2B signal sequence (aa1-31) and SEP (supereclip…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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pJFRC206-10XUAS-FRT>STOP>FRT-myr::smGFP-V5

Plasmid

#63168

Purpose

GAL4-responsive UAS “flp-On” spaghetti monster GFP-V5 reporter

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"spaghetti monster GFP-V5"

Use

Tags

N-myristoylation

Expression

Insect

Mutation

Promoter

Available since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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pCI-SEP_NR2A

Plasmid

#23997

Depositor

Robert Malinow

Article

Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

Insert

NR2A (Grin2a Rat)

Use

Tags

SEP

Expression

Mammalian

Mutation

Promoter

Available since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO ChETA-EYFP

Plasmid

#26968

Purpose

AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by EF1a promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi

Has Service

AAV1, AAV5, and AAV9

Insert

hChR2(E123T/H134R)-EYFP

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

E123T, H134R

Promoter

EF1alpha

Available since

Jan. 28, 2011

Availability

Academic Institutions and Nonprofits only

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Adeno Cas9

Plasmid

#64072

Purpose

Adenoviral vector for the expression of the Cas9

Depositor

Andrea Ventura

Article

Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

Type

Empty backbone

Use

Adenoviral

Tags

FLAG

Expression

Mutation

Promoter

U6 and CBh

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pJFRC201-10XUAS-FRT>STOP>FRT-myr::smGFP-HA

Plasmid

#63166

Purpose

GAL4-responsive UAS "flp-On" spaghetti monster GFP-HA reporter

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"spaghetti monster GFP-HA"

Use

Tags

N-myristoylation

Expression

Insect

Mutation

Promoter

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-C1C2-TS-EYFP

Plasmid

#35520

Depositor

Karl Deisseroth

Article

Kato et al Nature. 2012 Jan 22. doi: 10.1038/nature

Insert

ChR1-ChR2 Chimera

Use

Lentiviral

Tags

Trafficking signal and EYFP

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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Fuw CBX8

Plasmid

#121111

Purpose

conditional expression

Depositor

Emily Dykhuizen

Article

Connelly et al Nucleic Acids Res. 2018 Dec 29. pii: 526

Insert

CBX8 (CBX8 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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p006-GFP-Logic-AND

Plasmid

#112237

Purpose

GFP from jellyfish Aquorea sp. under D61 AND gate-like ara+lac promoter with AraC repressor; lacI repressor; kanamycin resistant.

Depositor

Jaroslaw Bryk

Article

Siddall et al bioRxiv 2021.06.20.449138

Inserts

GFP under control of doubly-repressible promoter
GFP
araC

Use

Tags

Expression

Bacterial

Mutation

Promoter

AND-gate and pLacI

Available since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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tubP-FRT>GAL80-6-FRT>

Plasmid

#63173

Purpose

ubiquitous expression of a "flp-Out" Drosophila codon-optimized GAL80 gene cassette

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"Flp-Out" GAL80

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pV132-vCas9

Plasmid

#203223

Purpose

Mammalian expression of vCas9

Depositor

Phil Sharp, Robert Langer, Vikash Chauhan

Article

Chauhan et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

Insert

vCas9

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

S55R R976A K1003A T1314R

Promoter

CBh

Available since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pmCherry-Akt2(WT)

Plasmid

#86623

Purpose

Expresses mCherry-tagged human Akt2(WT)

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt2 (AKT2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pLG1-puro Non-targeting sgRNA 3

Plasmid

#109003

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

Non-targeting sgRNA #3

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6 promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pKLV-EF1aGFP-W

Plasmid

#159291

Purpose

Lentiviral vector expressing GFP driven by human EF1a promoter

Depositor

Kosuke Yusa

Article

Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

human EF1a promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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TRF2-HA

Plasmid

#200209

Purpose

Expression of full length mTRF2

Depositor

Sheila Teves

Article

Kwan et al Elife. 2023 Mar 30;12:e83810. doi: 10.75

Insert

TRF2-HA (Terf2 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

None

Promoter

EF_1alpha

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pLG1-puro Non-targeting sgRNA 1

Plasmid

#109002

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

Non-targeting sgRNA #1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6 promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-mouse Oct4

Plasmid

#21110

Depositor

Dean Tantin

Article

Kang et al Genes Dev. 2009 Jan 15. 23(2):208-22.

Insert

Oct4 (Pou5f1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2009

Availability

Academic Institutions and Nonprofits only
pCas5

Plasmid

#82378

Purpose

sgRNA targeting YFP expressed from pJ23119 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

PJ23119

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3-basic-Polr2a promoter_600bps

Plasmid

#21111

Depositor

Dean Tantin

Article

Kang et al Genes Dev. 2009 Jan 15. 23(2):208-22.

Insert

Polr2a promoter_600bps (POLR2A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2009

Availability

Academic Institutions and Nonprofits only
pCas2F

Plasmid

#82377

Purpose

dCas9 expressed from aTc-inducible promoter in acsA, F RBS: TGGGCG

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

dCas9

Use

Tags

Expression

Mutation

Promoter

PEZ3

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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