-
Plasmid#167919PurposeLentiviral vector for expressing U6 MS2-sgRNA cloning plasmidDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only
-
pSB700 lenti gRNA Blasto_zhang2.0
Plasmid#167912PurposeLentiviral vector for expressing U6 MS2-sgRNA cloning plasmidDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP
Plasmid#67991PurposeCRISPR gRNA expression vector with an improved l scaffold and puro/BFP markers without WPREDepositorInsertU6gRNA cassette with an improved scaffold, PGKpuro2ABFP cassette
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
IL1RN gRNA4
Plasmid#113133PurposeExpresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)DepositorInsertIL1RN guideRNA 4 (SpCas9 scaffold), co-expressed GFP (transfection marker)
UseAAV, Synthetic Biology, and TALENTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXPR_011
Plasmid#59702PurposeThis lentiviral vector can be used to assay Cas9 activity.DepositorInsertEGFP sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEn-Chimera
Plasmid#61432PurposeGateway-Entry vector containing AtU6-26 promoter and sgRNA backboneDepositorInsertAtU6-26:sgRNA
UseCRISPRTagsExpressionPlantMutationPromoterAtU6-26Available sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJYS2_crtYf
Plasmid#85544PurposeConstitutive transcription of crRNA of crtYf, Spectinomycin resistanceDepositorInsertcrRNA of crtYf
UseCRISPR; Shuttle vector corynebacterium glutamicum…TagsExpressionBacterialMutationPromoterAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lenti_MCP-VP64_Hygro
Plasmid#138458Purposelenti vector encoding the MS2-VP64 with a 2A Hygro resistance markerDepositorInsertvp64
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1AAvailable sinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti_MCP-LSD1_Hygro
Plasmid#138457Purposelenti vector encoding the MS2-LSD1 with a 2A Hygro resistance markerDepositorInsertLSD1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAC-CR7T-gRNA2.1-nlsBFP
Plasmid#170515PurposegRNA cloning vector containing a CR7T promoter, gRNA(2.1) scaffold, and a Ubi-mTagBFP-NLS marker; optimized for germline CRISPR.DepositorTypeEmpty backboneUseCRISPRTagsExpressionInsectMutationPromoterCR7TAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJYS2cm_crtYf
Plasmid#85543PurposeConstitutive transcription of crRNA of crtYf, Chloramphenicol resistanceDepositorInsertcrRNA of crtYf
UseCRISPR; Shuttle vector corynebacterium glutamicum…TagsExpressionBacterialMutationPromoterAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSAG1::CAS9-U6::sg290860-6
Plasmid#59855PurposeEncodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for CRISPR disruption of TGME49_290860.DepositorInsertCRISPR sg290860-6
UseCRISPR; ; toxoplasma gondiiTagsExpressionMutationPromoterAvailable sinceOct. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMG
Plasmid#138264PurposeYeast intergration vector for integration of dual reporter system at the URA3 locus. Dual reporter system contains constitutive mCherry and constitutive eGFP flanked by iCas9 sites.DepositorInsertseGFP flanked by iCas9 sites
mCherry
UseCRISPRTagsExpressionYeastMutationPromoterTef1Available sinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUDP003
Plasmid#101164PurposeE. coli/S. cerevisiae amdS shuttle vector allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterScTDH3Available sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEL089
Plasmid#137897PurposeiSTU-CRISPR/Cas12a HH-HDV structure crRNA insertion vector with OsCDPK2 intronDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEL088
Plasmid#137896PurposeiSTU-CRISPR/Cas12a DR-DR structure crRNA insertion vector with OsCDPK2 intronDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEL087
Plasmid#137895PurposeiSTU-CRISPR/Cas12a guide RNA unit insertion vector with OsCDPK2 intronDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-gRNA:Asip
Plasmid#60968PurposeExpression vector of rat Asip guide RNADepositorInsertAgouti signaling protein gRNA (Asip Rat)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
SaCas9_sgRNA_expression_in_pBluescript
Plasmid#122090PurposeU6 driven SaCas9 sgRNA expression vector for cloning own guidesDepositorInsertSaCas9 tracr (NEWENTRY Synthetic, S. pyogenes)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBUN501
Plasmid#50582PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Bar resistanceDepositorInsertszCas9D10A
gRNA scaffold
UseCRISPR; Plant expressionTags3xFLAG and NLSExpressionMutationCas9D10A nickase, was derived from the zCas9 and …PromoterAtU6-26p and Ubi1pAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only