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Addgene
Showing: 901 - 920 of 1572 results

  1. pCDNA5-FRT-TO-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11

    Plasmid
    #174646
    Purpose
    Optogenetic coupling of RAB11 to opto-kinesin to induce anterograde transport of recycling endosomes. Compatible with Flp-in TREX system.
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)-P2A-iLID-mCherry-RAB11
    Use
    Tags
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…
    Promoter
    CMV (with TetOn)
    Availability
    Academic Institutions and Nonprofits only
    174646-plasmid-map-sequence-id-346959

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  2. pLV-hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110)-IRES-Blast

    Plasmid
    #193139
    Purpose
    Expresses bicistronic P2A construct of C-CRL4Cdt2 reporter(hCdt1(1-100)ΔCy-mCherry-) and APC/C reporter (hGeminin(1-110)) with blasticidin resistance
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110) (CDT1, GMNN Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    193139-plasmid-map-sequence-id-380234

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  3. pCDNA5-FRT-TO-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11

    Plasmid
    #174647
    Purpose
    Optogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomes. Compatible with Flp-in TREX system.
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    SSPB(micro)-mVenus-GCN4-ppKin14Vib(816-1321)-P2A-iLID-mCherry-RAB11
    Use
    Tags
    Expression
    Mammalian
    Mutation
    SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…
    Promoter
    CMV (with TetOn)
    Availability
    Academic Institutions and Nonprofits only
    174647-plasmid-map-sequence-id-345296

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  4. LIR-wtloxP-TRE-CAG-Frt-red-puro-3xPA-Frt-GFP(mVenus)-KrasG12D-cMyc-SV40LT-IRES-KRABoff-PA-TRE-loxP257-RIR

    Plasmid
    #67277
    Purpose
    inducbile color change and cancer regulation: FlpO recombinase dependent expression of KrasG12D cMyc and SV40 large T antigen with doxycycline inducible downregulation through tetKRAB system.
    Depositor
    Jannik Elverløv-Jakobsen
    Article
    Callesen et al Mol Oncol. 2017 Nov;11(11):1616-1629. do
    Inserts
    first casette contain a red fluorescent protein (katushka) linked to puromycin resitance gene through an E2A site
    second cassette contains mVENUS-kras-cmyc-SV40LT-KRABoff
    Use
    Tags
    mTQ2 blue fluorescent and red flourescent gene ka…
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    67277-plasmid-map-sequence-id-121189

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  8. YC 3.6 Cameleon

    Plasmid
    #58182
    Purpose
    Localization: Cytosolic Ca Biosensor, Excitation: 433 / 515, Emission: 475 / 528
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    ECFP and Venus
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. 2MT-D2-CPV

    Plasmid
    #58183
    Purpose
    Localization: Mitochondria Ca Biosensor, Excitation: 433 / 515 , Emission: 475 / 528
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    ECFP and Venus
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. 4MT-D3-CPV

    Plasmid
    #58184
    Purpose
    Localization: Mitochondria Ca Biosensor, Excitation: 433 / 515 , Emission: 475 / 528
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    ECFP and Venus
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. pJ151-HDR

    Plasmid
    #113631
    Purpose
    Contains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.
    Depositor
    Alex Kentsis
    Inserts
    Left homology arm
    Puromycin resistance gene
    Venus fluorescence gene
    Thymidine kinase
    Right homology arm
    Use
    Cre/Lox; Homology recombination selection vector
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    EF1a promoter, EF1a promoter (T2A cleaving sequen…
    Availability
    Academic Institutions and Nonprofits only
    113631-plasmid-map-sequence-id-222216

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  17. pBS7

    Plasmid
    #83774
    Purpose
    Useful for tagging yeast proteins with C-terminal Venus tag; G418 selection
    Depositor
    Eric Muller
    Article
    Hailey et al Methods Enzymol. 2002;351:34-49.
    Insert
    Venus
    Use
    Tags
    Venus
    Expression
    Yeast
    Mutation
    F46L, F64L, S65G, V68L, Q69M, S72A, Q80R, M153T, …
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    83774-plasmid-map-sequence-id-159984

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  19. PTEN-VC

    Plasmid
    #89332
    Purpose
    expresses hPTEN fused to the C-terminal part of Venus
    Depositor
    Tiago Outeiro
    Insert
    PTEN1 (PTEN Human)
    Use
    Tags
    C-terminal part of Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    89332-plasmid-map-sequence-id-176937

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Showing: 901 - 920 of 1572 results