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Plasmid#32237DepositorInsertN-cadherin (Cdh2 Mouse)
UseTagsEYFPExpressionMammalianMutationGlu–Glu–Asp (aa 780–782) ---> Ala–Ala–AlaPromoterCMVAvailable sinceOct. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-MIS12-LAP-BUB1
Plasmid#115904PurposeUsed for expression of siRNA-resistant MIS12-BUB1 from the FRT site of FlpIn cells upon addition of doxycyclin.DepositorInsertMIS12-BUB1 (MIS12 Human)
UseTagsEYFPExpressionMammalianMutationBUB1 fused to MIS12PromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
8xCTS1_BpiI-cloning-site_SV40polyA
Plasmid#124547PurposeDestination cloning vector for tRNA-flanked sgRNA. A BpiI placeholder is present between a minimal ADE promoter and SV40 polyA sequence.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
1114H
Plasmid#200640PurposeAn. gambiae transgenesis plasmid. Expresses gRNAs targeting B2-tubulin, ZPG, and DSXF. Crossing to Cas9 generates sterile malesDepositorInsertgRNA targeting DSXF, ZPG, and B2-tubulin. Actin5c-CFP and Vasa2-EYFP reporters.
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV/CaMKIIa::iLMO2
Plasmid#129481PurposeAAV2 transfer plasmid for iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemical, under control of the glutamatergic-selective CaMKIIa promoterDepositorInsertiLMO2
UseAAVTagsExpressionMammalianMutationPromoterCaMKIIaAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC0.161
Plasmid#119620PurposeLevel 0 Part. CDSDepositorInserteYFP C-term tag
UseSynthetic BiologyTagsExpressionMutationbp 242 T to APromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.009
Plasmid#119619PurposeLevel 0 Part. CDSDepositorInserteYFP+deg-tag
UseSynthetic BiologyTagsExpressionMutationbp 242 T to APromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
SarI T39N YFP
Plasmid#128155PurposeExpression of Sar1 with T39N dominant negative mutationDepositorInsertSar1
UseTagsEYFPExpressionMammalianMutationT39NPromoterAvailable sinceNov. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-shRNA_Tet3
Plasmid#85740PurposeshRNA against mouse Tet3DepositorInsertTet3 (Tet3 Mouse)
UseAAVTagsEYFPExpressionMammalianMutationPromoterAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pl-Synapsin-YFP-NaVII-III
Plasmid#91246PurposeExpresses YFP-NaVII-III from the Synapsin promoter, for neuron-specific and activity-independent live label of the Axon Initial Segment (AIS)DepositorInsertNav1.2 (Scn2a Rat)
UseLentiviralTagsYFPExpressionMammalianMutationThe insert codes only for the intracellular loop …PromoterAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ch-gelsolN
Plasmid#37262DepositorInsertgelsolin (GSN Human)
UseTagsmCherryExpressionMammalianMutationdeleted amino acids 380-731PromoterAvailable sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
YF-Cdc42(CA)
Plasmid#20152DepositorInsertYFP-FKBP-Cdc42(CA) (CDC42 Human)
UseTagsExpressionMammalianMutationCdc42H was inserted into the multiple cloning sit…PromoterAvailable sinceMarch 26, 2009AvailabilityAcademic Institutions and Nonprofits only -
5HT6-YFP-Inpp5e(D477N)
Plasmid#96809Purposephosphatase-dead control for 5HT6-YFP-Inpp5e, CAAX-nullDepositorInsert5HT6-YFP-Inpp5e(D477N)
UseTagsYFPExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
YFP-FKBPx5
Plasmid#103775Purposeexpresses iPOLYMER component in mammalian cellsDepositorInsertYFP-FKBPx5
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 gp78fl
Plasmid#37375DepositorInsertgp78 (AMFR Human)
UseTagsEYFPExpressionMammalianMutationP179L and F262LPromoterAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
8xCTS1_WT-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_WT-hutRNApro_SV40polyA
Plasmid#124546PurposeCloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.DepositorInsertWT-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_WT-hutRNApro
UseTagsExpressionMammalianMutationWT, WTPromoterMinimal ADEAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVPS13D-I-EGFP
Plasmid#176464Purposeexpress internally GFP-tagged VPS13D in mammalian cellsDepositorInsertVPS13D (VPS13D Human)
UseTagsGFP-UbExpressionMammalianMutationEYFP is internally insertedPromoterCMVAvailable sinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3XFlag-Mettl4
Plasmid#86665PurposeExpresses murine Mettl4 containing N-terminal 3XFlag tagDepositorInsertMettl4 (Mettl4 Mouse)
UseTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
5HT6-YFP-Inpp5e
Plasmid#96808Purposecilia-membrane-targeted catalytic domain of inositol phosphae 5-phosphatase, CAAX-nullDepositorInsert5HT6-YFP-Inpp5e
UseTagsYFPExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
5HT6-YFP-Inp54p(D281A)
Plasmid#96813Purposephosphatase-dead control for 5HT6-YFP-Inp54pDepositorInsert5HT6-YFP-Inp54p(D281A)
UseTagsYFPExpressionMammalianMutationD281A inactivates phosphatase activityPromoterAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only