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Plasmid#200341PurposemScarlet BioPart for PVP neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[PVPp | wrmScarlet | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterWBGene00003840, ocr-3Available sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSAH77 - [AWBp | wrmScarlet | tbb-2 UTR]
Plasmid#200351PurposemScarlet BioPart for AWB neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[AWBp | wrmScarlet | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterWBGene00005701, sru-38Available sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Bla/mCardinal-TurboID-GAI
Plasmid#198218PurposeTransposon vector (Addgene #60510) for doxycycline-inducible expression of a trimer with mCardinal, part of TurboID (after G257), and GAI (AAs 11-93) for gibberellic acid-inducible biotin labelingDepositorInsertmCardinal
UseTransposonTags3’ part of TurboID, starting with G257 and amino …ExpressionMammalianMutationNonePromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-mCardinal-P2A-GFP
Plasmid#197167PurposeExpresses the protein of mCardinal-P2A-GFP in mammalian cellsDepositorInsertmCardinal-P2A-GFP
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-fDIO-mScarlet-Gphn_C4a
Plasmid#194973PurposeAAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform C4a) under the control of human Synapsin promoterDepositorInsertmScarlet-Gphn (isoform C4a) (Gphn Synthetic, Rat)
UseAAVTagsmScarletExpressionMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-fDIO-mScarlet-Gphn_C4d
Plasmid#194975PurposeAAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform C4d) under the control of human Synapsin promoterDepositorInsertmScarlet-Gphn (isoform C4d) (Gphn Synthetic, Rat)
UseAAVTagsmScarletExpressionMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-fDIO-mScarlet-Gphn_P1
Plasmid#194972PurposeAAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform P1) under the control of human Synapsin promoterDepositorInsertmScarlet-Gphn (isoform P1) (Gphn Synthetic, Rat)
UseAAVTagsmScarletExpressionMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-fDIO-mScarlet-Gphn_C4c
Plasmid#194974PurposeAAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform C4c) under the control of human Synapsin promoterDepositorInsertmScarlet-Gphn (isoform C4c) (Gphn Synthetic, Rat)
UseAAVTagsmScarletExpressionMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFR-mScarletI-antiFRET-P2A-G-Ca-FLITS
Plasmid#191457PurposeCompare the intensity of the genetically encoded calcium sensor (GECI) G-Ca-FLITS to mScarlet-I fluorescent proteinDepositorInsertG-Ca-FLITS
UseTagsHis-mScarletI-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFR-mScarletI-antiFRET-P2A-EGFP
Plasmid#191458PurposeCompare the intensity of EGFP to mScarlet-I fluorescent proteinDepositorInsertEGFP
UseTagsHis-mScarletI-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFR-mScarletI-antiFRET-P2A-mTurquoise2_T203Y
Plasmid#191459PurposeCompare the intensity of mTurquoise2_T203Y to mScarlet-I fluorescent proteinDepositorInsertmTurquoise2_T203Y
UseTagsHis-mScarletI-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFR-mScarletI-antiFRET-P2A-GCaMP3
Plasmid#191460PurposeCompare the intensity of the genetically encoded calcium sensor (GECI) GCaMP3 to mScarlet-I fluorescent proteinDepositorInsertGCaMP3
UseTagsHis-mScarletI-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDRESS-mScarletI-antiFRET-P2A2-linker_noSacI
Plasmid#191473PurposeNegative control for ratiometric comparison between green fluorescent proteins and sensorsDepositorInsertnone
UseTagsHis-mScarletI-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFR-mScarletI-antiFRET-P2A-jGCaMP7c
Plasmid#191461PurposeCompare the intensity of the genetically encoded calcium sensor (GECI) jGCaMP7c to mScarlet-I fluorescent proteinDepositorInsertjGCaMP7c
UseTagsHis-mScarletI-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Kozak-mScarlet-I-RfA
Plasmid#186383PurposeDestination vector for the expression of a fluorescent fusion of N-ter mScarlet-I and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTR-454 tNGFR-P2A-CARD11
Plasmid#186081PurposeKnockin of truncated NGFR to target geneDepositorInsertCARD11 (CARD11 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
p159-RV-syn-dio-mScarlet-T2A
Plasmid#185700PurposeRetroviral vector expression cre-dependent membrane tagged mScarlet-T2A driven by the rat Synapsin promoter (with XhoI and PacI at 3'end of T2A to clone a gene of interest)DepositorInsertmScarlet
UseRetroviralTagsmScarlet palmitoylation with T2A on 3'-endExpressionMutationPromoterSynapsinAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hsChRmine-oScarlet-WPRE
Plasmid#183520PurposeOptogeneticsDepositorInserthsChRmine-oScarlet
UseAAVTagsExpressionMutationH33RPromoterCaMKIIaAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A CARD8-FLAG F405G
Plasmid#169956PurposeMammalian expression of CARD8-FLAG with a site III mutation (F405G)DepositorInsertCARD8 (CARD8 Human)
UseTagsFLAGExpressionMammalianMutationF405GPromoterCMVAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mScarlet-F-Ova-BlastR [M1G]
Plasmid#171817PurposeUniversal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and blasticidin resistance cassette [p.M1G]DepositorInsertmScarlet-F-Ova-T2A-BlastR
UseGene taggingTagsExpressionMutationBlastR: Changed Methionin 1 to Glycine.PromoterAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only