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Plasmid#141209PurposeTunable and temporal expression control of Cas9DepositorInsertCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG_NLS-Cas9-XTEN-Ec73RT-NLS_pA_pCMV_BFP_pA
Plasmid#176458PurposeVector for encoding a human codon-optimized SpCas9-XTEN-Ec73RT fusion driven by CAG promoter and tagBFP driven by CMV promoterDepositorInserthumanized S. pyogenes Cas9-XTEN-Ec73RT fusion
UseCRISPRTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti Vp64-dCas9 T2A mTagBFP2
Plasmid#171052PurposeExpression of Vp64-dCas9 activation systemDepositorInsertVp64-dCas9 T2A mTagBFP2
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA
Plasmid#178868PurposeMammalian expression of a protein of interest fused to the C-terminus of PB1-AzamiGreen-FRB-TEVcs-mTagBFP-HADepositorInsertPB1-AzamiGreen-FRB-TEVcs(ENLYFQL)-mTagBFP2-HA
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-mTagBFP2-T2A-iCre-ERT2
Plasmid#128168PurposeMonomeric TagBFP2 and iCre-ERT2 recombinase (PAPGSTMA N-terminus)DepositorInsertmTagBFP2-T2A-iCreERT2
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNASAM(gEmpty)-TREGFP-PGKpuroBFP-W
Plasmid#112926PurposeCRISPR-a reporter assay lentiviral vector (empty gRNA control)DepositorInserthU6-gRNASAM(empty), TREGFP, PGKpuroGFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAF0286 Ef1a-Cp36_attB-BFP acceptor
Plasmid#193474PurposeAcceptor plasmid for 3 plasmid recombination assayDepositorInsertCp36_attB
UseTagsExpressionMammalianMutationPromoterEf1aAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
hGFAP-NLS-HA-Dre-P2A-BFP
Plasmid#51277PurposeCo-expression of the site-specific recombinase Dre and BFP under the control of the hGFAP promoterDepositorInsertNLS-HA-Dre-P2A-BFP
UseTagsExpressionMutationPromoterhGFAPAvailable sinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(BsmBI)-EF1a-BFP-BSD
Plasmid#117136PurposeEmpty vector encoding BFP-BSDDepositorInsertBSD2A
UseLentiviralTagsExpressionMammalianMutationmodified BFPPromoterhuman U6 promoter, human EF1a promoterAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCCL-PGK-SPdCas9-BFP-DNMT1
Plasmid#66818PurposedCas9 fused to BFP and the human DNMT1 catalytic domainDepositorInsertdCas9-BFP-DNMT1, catalytic domain (DNMT1 )
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCCL-PGK-SPdCas9-BFP-DNMT3A
Plasmid#66819PurposedCas9 fused to BFP and the human DNMT3A catalytic domainDepositorInsertdSpCas9-BFP-DNMT3A, catalytic domain of DNMT3A
UseTagsdSpCas9-BFP-DNMT3AExpressionMammalianMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A STING-354STOP
Plasmid#102589PurposeLentivector to express STING delta 354 and mTagBFPDepositorInsertSTING (STING1 Human)
UseLentiviralTagsExpressionMammalianMutationdelta 354PromoterSFFVAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-gal4-VP16-gcry-TagBFP
Plasmid#117777PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertgal4/VP16
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-gal4-VP16-cmlc2-TagBFP
Plasmid#117780PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertgal4/VP16
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
SL2-sgTelo-MTSa/BFP/pdCas9-C1
Plasmid#162760PurposeExpressing dCas9 and sgRNA containg MTSa targeting telomeresDepositorInsertdCas9 and sgRNA(SL2-sgTelo-MTSa)
UseTagsExpressionMammalianMutationdCas9(nuclease deactivated Cas9)PromoterU6/CMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-mTagBFP2-T2A-iCreERT2 (PAGSAS)
Plasmid#137864PurposeMonomeric TagBFP2 and iCre-ERT2 recombinase (PAGSAS N-terminus)DepositorInsertmTagBFP2-T2A-iCreERT2
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-DD-3xFLAG-hSOX17-GS-TagBFP-BGHpA
Plasmid#172226PurposeExpression vector with Shield-1 stabilizable hSOX17 fused to TagBFP.DepositorInsertSOX17 (SOX17 Human)
UsePiggybac transposonTags3XFLAG, DD-domain, GS-linker, and TagBFPExpressionMammalianMutationPromoterCAGAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAd/CMV/PBX1-IRES-nEBFP2
Plasmid#29755DepositorInsertPre-B-cell leukemia transcription factor 1 (PBX1 Human)
UseTagsEBFP2, IRES, and Nuclear localization sequenceExpressionMammalianMutationNo mutations; EBFP2 is nuclear localized; PBX1 an…PromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
SL2-sgSat-MTSb/BFP/pdCas9-C1
Plasmid#162761PurposeExpressing dCas9 and sgRNA containg MTSa targeting centromeresDepositorInsertdCas9 and sgRNA(SL2-sgSat-MTSb)
UseTagsExpressionMammalianMutationdCas9(nuclease deactivated Cas9)PromoterU6/CMVAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CAG-NCreIntN-EF1a-mTagBFP2
Plasmid#160507PurposeExpresses NCreIntN in mammalian cells in split intein-mediated split-Cre recombinase applicationsDepositorInsertNCreIntN
UseCre/Lox and LentiviralTagsmTagBFP2ExpressionMammalianMutationPromoterCAGAvailable sinceJan. 22, 2022AvailabilityAcademic Institutions and Nonprofits only