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Plasmid#67089PurposeStandard for cell-free expression benchmarking.DepositorInsertGTF2E1 (GTF2E1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSN17
Plasmid#100104Purposeexpresses a chimeric OCT4-repressor fusionDepositorUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
aL-L3
Plasmid#103881Purposefull-length human aL with moxsynGFP in betapropellerDepositorInsertfull-length human aL with moxsynGFP in betapropeller (ITGAL Human)
UseTagstension sensor moduleExpressionMutationPromoterAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-POLArISact
Plasmid#164970PurposeT7 promotor drives in vitro transcription of POLArISact with a human kozak sequenceDepositorInsertPOLArISact
UseIn vitro transcriptionTagsExpressionMutationPromoterT7Available sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAGGS-mNAGA
Plasmid#196448PurposeExpresses modified α-N-acetylgalactosaminidase in mammalian cellsDepositorInsertmodified alpha-N-acetylgalactosaminidase (NAGA Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
MigR1-Flag-TRIB2
Plasmid#131154PurposeRetroviral expression of flag-tagged mouse TRIB2DepositorInsertTrib2 (Trib2 Mouse)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
MigR1-Flag-TRIB1
Plasmid#131158PurposeRetroviral expression of flag-tagged mouse TRIB1DepositorInsertTrib1 (Trib1 Mouse)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
MigR1-Myc-Nterm TRIB2
Plasmid#131153PurposeRetroviral expression of N terminus of mouse TRIB2DepositorInsertTrib2 (Trib2 Mouse)
UseRetroviralTagsMycExpressionMammalianMutationcontains amino acids 1-145PromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
MigR1-Flag-TRIB3
Plasmid#131159PurposeRetroviral expression of flag-tagged mouse TRIB3DepositorInsertTrib3 (Trib3 Mouse)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBB14
Plasmid#18657DepositorInsertUs9-EGFP gene fusion
UseTagsEGFPExpressionMammalianMutationFirst 106 amino acids of Us9 are intact. The stop…PromoterAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
POLArISact
Plasmid#164971PurposeExpresses POLArISact, an orientation probe that specifically binds to F-actin, in mammalian cellsDepositorInsertPOLArISact
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
human a5 full length
Plasmid#217818PurposeFull length human integrin a5 expressionDepositorInsertITGA5 (ITGA5 Human)
UseTagsP2A-EGFPExpressionMammalianMutationPromoterCAGAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
mouse a2b full length
Plasmid#217812PurposeFull length mouse integrin a2b expressionDepositorInsertITGA2B (Itga2b Mouse)
UseTagsP2A-EGFPExpressionMammalianMutationPromoterCAGAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
mouse av full length
Plasmid#217809PurposeFull length mouse integrin av expressionDepositorInsertITGAV (Itgav Mouse)
UseTagsP2A-EGFPExpressionMammalianMutationPromoterCAGAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJA291
Plasmid#74487PurposeeF1alpha::puroR::mCherry::T2A::eGFP::MCS::wPRE for assaying effects of C-terminal fusions to eGFPDepositorInsertpuroR-mCherry-T2A-eGFP
UseLentiviralTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDownstream1Lox
Plasmid#164574PurposeIntegration of 1 LoxN site in the genome of P. bergheiDepositorTypeEmpty backboneUseCre/LoxTagsExpressionMutationPromoterBidirectional PbEF1alpha promoter (PBANKA_1133300…Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
cpVenus-Gγ2
Plasmid#69626PurposeThe heterotrimeric G-protein subunit, Gγ2, tagged with circular permutated Venus (cp173V).DepositorInsertcp173Venus-Ggamma2 (GNG2 Synthetic)
UseTagsciruclar permutated Venus (cp173V)ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
ss-ED-mCherry-GPI
Plasmid#163680PurposeExprsses the GPI-anchored luminal domain of ST6GAL1 with a mCherry tag in mammalian cellsDepositorInsertThe luminal domain of ST6 beta-galactoside alpha-2,6-sialyltransferase 1 with N-terminally tagged signal sequences (ST6GAL1 Human)
UseTagsGPI anchor, mCherry, and signal sequencesExpressionMammalianMutationThis chimera contains the ecto-domain of ST. Its …PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAP215.rAAV.mU6-sgRNA.Handle.EF1a-mTagBFP2
Plasmid#217635PurposeAAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.DepositorInsertmU6-sgRNA, Handle sequence, EF1a-mTagBFP2
UseAAVTagsExpressionMutationPromoterEF1alpha and mU6Available sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLCE-P2A-Puro
Plasmid#123318PurposeLentiviral vector for expression of eGFP-P2A-Puro cassette from a CMV promoterDepositorInserteGFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only