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pX330A-1x6
Plasmid#58770PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pG12-Rluc8
Plasmid#170272PurposeRenilla reniformisluciferase (RLuc8) expression in mammlian cells. Reporter plasmid.DepositorInsertRLuc8
UseTagsExpressionMammalianMutationPromoter12xUAS with TATA-box minimal promoterAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC1812_pCR8-gCasRx-CUG (control gRNA)
Plasmid#118645PurposeTransient transfection; Expressed Control (CUG repeat) CasRx gRNA; Gateway DonorDepositorInsertgCasRx-CUG
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pR26-CAG-H2B-miRFP703
Plasmid#112853PurposeDonor vector for targeting H2B-miRFP703 into Rosa26 locus to generate reporter mice or cell linesDepositorInsertH2B-miRFP703
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG506-mito-frFAST
Plasmid#160057PurposeExpresses mito-frFAST in mammalian cellsDepositorInsertMito-frFAST
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmMaple3-CAM
Plasmid#101148PurposeFluorescent protein for superresolution imagingDepositorInsertE. coli codon optimized mMaple3
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
TRPML2-MYC
Plasmid#18829DepositorInsertTRPML2 (Mcoln2 Mouse)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceJuly 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAd5-B6-deltaE3
Plasmid#122559PurposeModified block 6, with deletion in E3DepositorInsertAdenovirus 5 genomic region 27859-30803
UseSynthetic BiologyTagsExpressionMutationAdenovirus sequences 27859-30803 replaced with Ba…PromoterAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRi-D2
Plasmid#182927Purposeinducible CRISPRi plasmid with gRNA targeting to psbD gene in Synechocystis 6803DepositorInsertddcpf1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKSCTMF
Plasmid#85073PurposeGene insertion/deletion in Pichia pastorisDepositorInsertAOX1promoter - mazF - AOX1 tt- Zeocin R gene
UseGene insertion/deletion in pichia pastorisTagsExpressionMutationPromoterpAOX1Available sinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330A-1x5
Plasmid#58769PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-MPAct-mCit-IRES-Neo
Plasmid#155230Purposelentiviral MPAct constructDepositorInsertLentiviral expression of MPAct-mCitrine sensor
UseLentiviralTagsmCitrineExpressionMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGCDNsam-Foxa2
Plasmid#33004DepositorInsertforkhead box A2 (Foxa2 Mouse)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJH291
Plasmid#172153PurposeReporter plasmid for pylBCD variant in pCDF backboneDepositorInsertPara sfGFP.2TAG; Plpp pylS; PproK pylT; AraC
UseTagsExpressionBacterialMutationsfGFP(N39*, Y151*)PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
N-GFP-Dcp2
Plasmid#163585Purposeexpress N-terminal GFP tagged Dcp2 FL at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationPromoterDCP2 promoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX TIF1gamma
Plasmid#15734DepositorInsertTIF1 gamma (TRIM33 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
MO70 Orai1 N223A
Plasmid#21663DepositorInsertOrai1 (ORAI1 Human)
UseRetroviralTagsFlagExpressionMammalianMutationN223APromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRetroSuper-GFP Smad4
Plasmid#15724DepositorInsertSmad4 shRNA (SMAD4 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Sec61β
Plasmid#172445PurposeExpression of ER protein Sec61beta with an N-terminal mCherry tagDepositorInsertProtein transport protein Sec61 subunit beta
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
TRPML2-YFP
Plasmid#18830DepositorInsertTRPML2 (Mcoln2 Mouse)
UseTagsYFPExpressionMammalianMutationPromoterAvailable sinceJuly 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-HBH-kanMX6
Plasmid#26872DepositorInsertHBH tag
UsePcr tagging yeastTagsExpressionMutationPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pX330A-1x7
Plasmid#58771PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHACK-GAL4>QF2(newHA1)
Plasmid#104873PurposeHACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of insertsDepositorInsertT2A-QF2
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Venus-KRasCT
Plasmid#99016PurposeMembrane markerDepositorInsertVenus KRasCT
UseTagsExpressionMammalianMutationPromoterCMAvailable sinceJune 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc-mE6-AP/2591a
Plasmid#11468DepositorInsertmE6-AP (Ube3a Mouse)
UseTagsmycExpressionMammalianMutationn/aPromoterAvailable sinceMarch 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
mCherry-KRasCT
Plasmid#99017PurposeMembrane markerDepositorInsertmCherry KRas CT
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+) ChemoG1
Plasmid#193799PurposeProtein production of ChemoG1 in bacteriaDepositorInsertChemoG1
UseTags10xHisExpressionBacterialMutationPromoterT7Available sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKOdeltagalK (Kan)
Plasmid#110089PurposeAnalogous to pKO but without the negative selection gene galK.DepositorTypeEmpty backboneUseBacterial knockout generationTagsExpressionMutationPromoterAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT/TO MycLAP-STIL
Plasmid#80266Purposemammalian expression plasmid for MycLAP-STILDepositorInsertSTIL (STIL Human)
UseTagsMyc-GFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Halo-Erk2 donor
Plasmid#113104PurposeDonor plasmid for knock-in HaloTag fusing to the N-terminal of mouse Erk2 coding sequenceDepositorInsertN terminal Erk2 donor region including homology arms and HaloTag
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
PBS Actb-2A-TIR1-GFP
Plasmid#113832Purposetargeting osTIR1-GFP into mouse Actb locus for auxin inducible degradationDepositorInsertosTIR1-GFP (NEWENTRY Synthetic)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-siRNA control
Plasmid#10900DepositorInsertsiGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_human_spike-in
Plasmid#125152PurposeEmpty vector to clone and measure the expression of promoter candidates (spike-in) by determining the abundance of transcripts originating from the candidate in human cellsDepositorTypeEmpty backboneUseStap-seq screening vectorTagsExpressionMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6-2 SNAP-Syk tSH2
Plasmid#113028Purposefor purification of Syk tSh2. Purified protein will have a snap tag that can be conjugated to fluorescent dyes.DepositorInsertSNAP-Syk tSH2
UseTagsSNAPExpressionBacterialMutationPromoterAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP N1 Ub-CRT
Plasmid#69619PurposeExpress cytoplasmic mature CRT.DepositorInsertUbiquitina-Calreticulin (mature)
UseTagsEGFP and UbiquitinExpressionMammalianMutationDeletion of signal peptide of calreticulin at the…PromoterCMVAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAd5-B7-deltaE4
Plasmid#122560PurposeModified block 7, with deletion in E4DepositorInsertAdenovirus 5 genomic region 30888-35938
UseSynthetic BiologyTagsExpressionMutationAdenovirus sequences 32874-35461 replaced with Sp…PromoterAvailable sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm-REP
Plasmid#64966Purposemini-Tn7 vector with oriR6K, for attTn7 site determinationDepositorInsertoriR6K
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGKO2-Gateway
Plasmid#63618Purposea binary Agrobacterium vector carrying a modified HSVtk gene as a negative selection marker against ectopic transformants. Uses Gateway cloning.DepositorTypeEmpty backboneUseFungal expressionTagsExpressionMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30-T7-sfGFP
Plasmid#180757PurposepET30 vector missing lacO and N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminatorDepositorInsertsuperfolder green fluorescent protein
UseTagsstrep tagExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH462
Plasmid#172154PurposepylBCD variant in pBAD backboneDepositorInsertPara (Sumo-tagged pylBCD); AraC
UseTagsExpressionBacterialMutationSUMO tag fused to N-terminus of PylBPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Hbxadr-Hatag
Plasmid#24930DepositorInsertHBx
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJune 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLIA-E p27 (CC#620)
Plasmid#15191DepositorInsertp27 (Cdkn1b Mouse)
UseRetroviralTagsFlag and HisExpressionMammalianMutationPromoterAvailable sinceJune 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pPEE17
Plasmid#128361PurposeDetecting gene essentiality in Cryptococcus neoformans. Targets DNA constructs to the Safe Haven Site 1, a small gene-free region. Contains amdS2 counterselectable marker. This version of the Safe Haven vector includes direct repeats, enabling the formation of popouts on fluoroacetamide.DepositorInsertAcetamidase (amdS)
UseUnspecifiedTagsExpressionMutationPromoterTEF1Available sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMEXC3H
Plasmid#110080PurposeAnalogous to pMINTC3H, but containing oriM for episomal replication in mycobacteria.DepositorTypeEmpty backboneUseTags3C cleavage site - His10 TagExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCJH065_proD_Cas12c_DualRNA-GFP+HDVrz_CAM
Plasmid#183077PurposePlasmid expressing Cas12c tracrRNA and GFP-targeting crRNA separately, with the crRNA ending with a HDV ribozyme sequence.DepositorInsertCas12c tracrRNA and crRNA ending with a HDV ribozyme
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pSCW01
Plasmid#72300PurposeStarting substrate for in vitro DNA mismatch repair substrate monitored by PstI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.DepositorTypeEmpty backboneUseDerivative of puc19TagsExpressionBacterialMutationPromoterlacAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKDH
Plasmid#67584PurposeGene expression in plants. A promoter and/or reporter can be cloned into this plasmidDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterNoneAvailable sinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSJ111
Plasmid#78707PurposeModular vector for constitutive secreted amylase reporter expressionDepositorInsertamyEopt
UseTagsExpressionBacterialMutationPromoterPvegAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only
