Skip to main content
Addgene
Showing: 2251 - 2300 of 6831 results

  1. pAG32-L-IFP-F[2] (clon 3)

    Plasmid
    #52900
    Purpose
    Plasmid template for PCR of cassettes for HR, used to introduce IFP F[2] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.
    Depositor
    Stephen Michnick
    Article
    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:
    Insert
    L-IFP-F[2]
    Use
    Vector for homologous recombination
    Tags
    Expression
    Mutation
    Promoter
    TEF promoter
    Available since
    June 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  2. pMSCVpuroATT

    Plasmid
    #47539
    Purpose
    Retroviral Gateway destination vector constructed by inserting Reading Frame Cassette A into pMSCVpuro (Clontech) using the Gateway Vector Conversion System (Invitrogen)
    Depositor
    David Weinstock
    Article
    Shindoh et al PLoS One. 2012;7(11):e49201. doi: 10.137
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 25, 2013
    Availability
    Academic Institutions and Nonprofits only

  7. pBlastR-5xUAS-sfGFP-GMRWhite

    Plasmid
    #165907
    Purpose
    Blasticidin resistant 5xUAS sfGFP GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Insert
    Selectable UAS Response Vector
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    165907-plasmid-map-sequence-id-326309

    Enlarge

  12. P[acman]-Omega2

    Plasmid
    #165861
    Purpose
    Omega2 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screening
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    165861-plasmid-map-sequence-id-336788

    Enlarge

  39. pBlastR-5xUAS-sfGFP-MiniWhite

    Plasmid
    #165910
    Purpose
    Blasticidin resistant 5xUAS sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Insert
    Selectable UAS Response Vector
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    165910-plasmid-map-sequence-id-329667

    Enlarge

  43. pFA0055

    Plasmid
    #131774
    Purpose
    Guide RNA (gCASS5a) and Cas9 expression plasmid for cleaving pFA6 series deletion cassettes, including KanMX, hphMX and natMX. Used to perform CRISPR-Swap of alleles.
    Depositor
    Frank Albert
    Article
    Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.
    Insert
    20mer CASS5a guide (gCASS5a) and 5' sgRNA
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Oct. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    131774-plasmid-map-sequence-id-258731

    Enlarge

  44. dTomato_LD 0_CS G_tTA-BFP

    Plasmid
    #58871
    Purpose
    MESA target chain with dTomato ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA target chain with dTomato ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
    Use
    AAV
    Tags
    EBFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58871-plasmid-map-sequence-id-94232

    Enlarge

Showing: 2251 - 2300 of 6831 results