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pET_3E2H.37

Plasmid

#155211

Purpose

Expresses 3E2H.37 de novo designed protein in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

3E2H.37

Use

Tags

6x His Tag

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIA1(T16A)-NLS(SV40) (KAC511)

Plasmid

#133797

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1(T16A) with C-terminal NLS (SV40)

Depositor

Benjamin Kleinstiver, Joseph Bondy-Denomy

Article

Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

Insert

human codon optimized AcrIIA1(T16A)

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

T16A

Promoter

CMV and T7

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIA1-Leu-NLS(SV40) (KAC440)

Plasmid

#133799

Purpose

CMV and T7 promoter expression plasmid for human codon optimized Leu-AcrIIA1 with C-terminal NLS (SV40)

Depositor

Benjamin Kleinstiver, Joseph Bondy-Denomy

Article

Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

Insert

human codon optimized Leu-AcrIIA1

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

n/a

Promoter

CMV and T7

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIA1-Eriv-NLS(SV40) (KAC442)

Plasmid

#133800

Purpose

CMV and T7 promoter expression plasmid for human codon optimized Eriv-AcrIIA1 with C-terminal NLS (SV40)

Depositor

Benjamin Kleinstiver, Joseph Bondy-Denomy

Article

Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

Insert

human codon optimized Eriv-AcrIIA1

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

n/a

Promoter

CMV and T7

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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tol2-lyzC-miR-23a-2-dendra2

Plasmid

#105435

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

miR-23a-2 Dendra2 (mir23a-2 Zebrafish)

Use

Zebrafish

Tags

N/A

Expression

Mutation

Promoter

Available since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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hsa-cdk2 3'UTR sicheck2

Plasmid

#121125

Purpose

luciferase assay

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

hsa-cdk2 3'UTR (CDK2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSB139 - pL0_ZCT1 (CDS1)

Plasmid

#123184

Purpose

Golden Gate (MoClo; CDS1) compatible ZCT1 gene from Catharanthus roseus; a repressor of the STR1 promoter from C. roseus

Depositor

Carolyn Lee-Parsons, Erin Cram

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

ZCT1 from Catharanthus roseus

Use

Part for plant expression

Tags

Expression

Plant

Mutation

No BpiI and BsaI sites

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSB142 - pL0_ORCA3 (CDS1)

Plasmid

#123185

Purpose

Golden Gate (MoClo; CDS1) compatible ORCA3 gene from Catharanthus roseus; an activator of the STR1 promoter from C. roseus

Depositor

Carolyn Lee-Parsons, Erin Cram

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

ORCA3 from Catharanthus roseus

Use

Part for plant expression

Tags

Expression

Plant

Mutation

No BpiI and BsaI sites

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSB62 - pL0_STR (pro + 5U)

Plasmid

#123186

Purpose

Golden Gate (MoClo; PRO + 5U) compatible STR1 promoter from Catharanthus roseus

Depositor

Carolyn Lee-Parsons, Erin Cram

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

Catharanthus roseus STR1 promoter and 5'UTR

Use

Part for plant expression

Tags

Expression

Plant

Mutation

No BpiI and BsaI sites

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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AAV CMV-driven AcrIIC1-scaffold (2xBsmBI sites)

Plasmid

#120300

Purpose

AAV vector for expression of AcrIIC1 (no miR binding sites, control vector)

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

AcrIIC1

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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AAV CMV-driven AcrIIC1-2xmiR-122 target sites

Plasmid

#120302

Purpose

AAV vector for expression of AcrIIC1 with two miR-122 binding sites

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

AcrIIC1-2xmiR-122 binding sites

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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AAV CMV-driven AcrIIC3-2xmiR-122 target sites

Plasmid

#120303

Purpose

AAV vector for expression of AcrIIC3 with two miR-122 binding sites

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

AcrIIC3-2xmiR-122 binding sites

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only
pACEBAC1-CoREST-C

Plasmid

#109159

Purpose

Encodes a human C-terminal CoREST fragment with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Inui et al Protein Sci. 2017 May;26(5):997-1011. do

Insert

human C-terminal CoREST, sequence-optimized for insect cells (RCOR1 Human)

Use

Tags

3xFLAG

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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Thy-C73A-HA

Plasmid

#111542

Purpose

For expression of human KCNB1 in mouse brain

Depositor

Federico Sesti

Article

Yu et al J Neurosci. 2016 Oct 26;36(43):11084-110

Insert

thy1.2-C73A-HA (KCNB1 Human)

Use

Mouse Targeting

Tags

HA

Expression

Mutation

C73A

Promoter

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1alpha-HA-TFAP4(E135A/S139A)

Plasmid

#110159

Purpose

Lentiviral vector for expression of human TFAP4 (E135A/S139A) in mammalian cells

Depositor

Daniele Guardavaccaro

Article

D'Annibale et al J Biol Chem. 2014 Mar 14;289(11):7730-7.

Insert

TFAP4 (transcription factor AP-4) (TFAP4 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

E135A/S139A

Promoter

CMV

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMflT-o3

Plasmid

#101311

Purpose

Contains: oriC3 fragment of M. florum L1 replication origin (rpmH/dnaA and dnaA/dnaN intergenic regions, w/o dnaA), colE1 rep origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.

Depositor

Sébastien Rodrigue

Article

Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

Insert

oriC3 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, w/o dnaA)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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CDk4-luciferase fragment B

Plasmid

#86659

Purpose

Luciferase reporter containing CDK4 promoter

Depositor

Jaime Modiano

Article

Baksh et al Mol Cell. 2002 Nov;10(5):1071-81.

Insert

CDK4 promoter (CDK4 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

generated by cleavage at the KpnI site from 880 (…

Promoter

CDK7

Available since

June 20, 2017

Availability

Academic Institutions and Nonprofits only

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MT79

Plasmid

#63112

Purpose

The artificial microRNA is designed for down-regulating expression of ACD6 mRNA.

Depositor

Detlef Weigel

Article

Todesco et al Nature. 2010 Jun 3;465(7298):632-6. doi:

Insert

amiR-ACD6

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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tdEos-Cx43-7

Plasmid

#57603

Purpose

Localization: Gap Junctions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Cx43

Use

Tags

tdEos

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pMSCV-puro-mMCL1 Delta C22

Plasmid

#45820

Depositor

Joseph Opferman

Article

Perciavalle et al Nat Cell Biol. 2012 Apr 29;14(6):575-83.

Insert

mMCL-1 Delta C22

Use

Tags

Expression

Mammalian

Mutation

C-terminal 22 amino acids are deleted from full l…

Promoter

Available since

July 24, 2013

Availability

Academic Institutions and Nonprofits only