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Plasmid#155119PurposeTol2 vector for ubiquitous expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis markerDepositorInsertubi:QFGal4 (ubb Zebrafish, Neurospora crassa, Budding Yeast)
UseZebrafish expressionTagsExpressionMutationPromoterubiquitin BAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SYN-H-sDarken
Plasmid#184797PurposeHigh affinity version of the Serotonin Sensor sDarken under the control of a hSyn Promoter.DepositorInsertH-sDarken
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
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HA-mDyn1 pcDNA3
Plasmid#36263DepositorInsertDynamin 1 (Dnm1 Mouse)
UseTagsHAExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceApril 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pIDK-HDAC1fl
Plasmid#109158PurposeEncodes human full-length HDAC1 to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman full-length HDAC1, sequence-optimized for insect cells (HDAC1 Human)
UseTagsExpressionInsectMutationPromoterp10Available sinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNICKclos1.0
Plasmid#73639PurposeGenome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824DepositorInsertsCas9 nickase
sGRNA to pyrE
UseE.coli-clostridium shuttle vectorTagsExpressionMutationD10APromoterj23119 and ptbAvailable sinceJune 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A mKate2-hu_ncOGT WT
Plasmid#154282PurposeEntry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; WT)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseGateway entry vectorTags2x FLAG and mKate2ExpressionMutationWT OGTPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pH-nCas9-PPE
Plasmid#140447PurposeFor plant prime editing in rice plants or monocotyledons protoplastsDepositorInsertnCas9(H840A)-M-MLV
UseCRISPRTagsExpressionPlantMutationH840A for Cas9; D200N, T306K, W313F, T330P and L…Promotermaize Ubiquitin-1, OsU3Available sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
HA-EglN3-pcDNA3
Plasmid#18960DepositorInsertEgg laying Nine - 3 (EGLN3 Human)
UseTagsHA-tagExpressionMammalianMutationwt cDNAPromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLM1110_LEU2
Plasmid#168460PurposeBig-IN Payload yeast assembly vector. Encodes LEU2 yeast selectable marker and components for bacterial copy number induction. Contains a mammalian transient (backbone) GFP-T2A-BSD selection cassette.DepositorInsertmRFP1
UseSynthetic Biology; Yeast assembly vector for big-…TagsExpressionMutationPromoterAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV-EF1a-mCherryLuciferase-W
Plasmid#200101PurposeLentiviral vector expressing mCherry fused with Luciferase at the C-terminusDepositorInsertLuciferase
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUltraI-Tet3.0[TAA]
Plasmid#164580PurposeMachinery plasmid containing a single copy of the Mb Tet3.0RS and cognate tRNA for incorporation of Tet3.0 at TAA codonsDepositorInsertsMb Tet3.0RS (R2-3)
Mm tRNA[TAA]
UseTagsExpressionBacterialMutationL170G, N311G, C313APromoterProK and TacIAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
PIK3CA - Exon9 E545K
Plasmid#16642DepositorInsertPIK3CA E545K (PIK3CA Human)
UseTagsFlagExpressionMammalianMutationE545K; see note belowPromoterAvailable sinceMarch 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
miniCGBE1-NG (pBM1182)
Plasmid#140257PurposeCMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_NG-P2A-EGFPDepositorInsertBE4max(R33A)_NG-ΔUGI
UseTagsExpressionMammalianMutationR33A in rAPOBEC1, NG mutations in SpCas9, D10A in…PromoterCMVAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSG5-hEKLF
Plasmid#67835PurposeExpression of human EKLF driven by SV40 promoter for use in activation studiesDepositorInsertEKLF (KLF1 Human)
UseTagsExpressionMammalianMutationPromoterSV40Available sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET19-MTdTc302
Plasmid#207458PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302DepositorInsertMTdTc302 (Dntt Mouse)
UseTags10xHis-MBPExpressionBacterialMutationCys188Ala, Cys216Ser, Cys378Ala, and Cys438SerPromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA pDEST40 Cas9-POLD3
Plasmid#183194PurposeExpression cloneDepositorInsertPOLD3
UseCRISPRTagsCas9WTExpressionMammalianMutationn/aPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A mKate2-hu_ncOGT K842M
Plasmid#154284PurposeEntry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; K842M variant, catalytic dead)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseGateway entry vectorTags2x FLAG and mKate2ExpressionMutationK842M mutation (Catalytic Inactive)PromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19.TRIM28.FKBP.knock-in.mRuby2
Plasmid#185008PurposeFor knocking in FKBP F36V P2A mRuby2DepositorInsertFKBP F36V P2A mRuby2
UseCloningTagsExpressionMutationPromoterAvailable sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-A3
Plasmid#135505PurposeMammalian expression of myc-tagged HLA-A*03:01DepositorInsertHLA-A*03:01 (HLA-A Human)
UseTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only