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Plasmid#100799Purposehelper virus for monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTADepositorHas ServiceAAV1InsertmTagBFP2 and rabies virus SAD B19 strain glycoprotein genes (B19G)
UseAAVTagsExpressionMammalianMutationPromoterTREtightAvailable sinceOct. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
6xHis-TagRFP-T_A1aY1
Plasmid#158754PurposeExpression of 6x His tagged TagRFP-T_A1aY1 in mammalian cells for detection of tyrosinated microtubules.DepositorInsert6xHistidine_TagRFP-T_A1aY1
UseTags6x-Histidine tag and TagRFP-TExpressionMammalianMutationPromoterCMV PromoterAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHS1859
Plasmid#205972PurposeFLAG-SV40 NLS-Csy3/Cas7 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)DepositorInsertCsy3/Cas7 from JAGBLH010000220, Cas8-HNH
UseTagsFLAG-SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1860
Plasmid#205973PurposeFLAG-SV40 NLS-Cas6/Csy4 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)DepositorInsertCas6/Csy4 from JAGBLH010000220, Cas8-HNH
UseTagsFLAG-SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
His6tev CBP HAT (1324-1700)
Plasmid#99340Purposebacterial expression of His tagged mouse CBP HAT domain (1324-1700)DepositorInsertmouse CBP HAT domain (1324-1700) (Crebbp Mouse)
UseTagsHis6tevExpressionBacterialMutationPromoterT7Available sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-rtTA2-TRE-SspB-mScarlet-I-MYPT169
Plasmid#178528PurposeExpresses rtTA and SspB-mScarlet-I-MYPT (1-169 aa) in mammalian cellsDepositorInsertrtTA, SspB-mScarlet-I-MYPT (1-169aa)
UseTagsExpressionMammalianMutationPromoterCAG, Tet responsive elementAvailable sinceDec. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK FKBP12(F36V)-OGT (Neo)
Plasmid#154294PurposeLentiviral vector encoding FKBP12(F36V)-2xHA-OGT (Neomycin resistant) off PGK promoterDepositorInsertO-GlcNAc Transferase (OGT Human)
UseLentiviralTags2x HA and FKBP12(F36V)ExpressionMammalianMutationPromoterPGKAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti_CMV-Cas9-P2A-HygR
Plasmid#164133PurposeLentiviral construct for the expression of SpCas9 driven by CMV promoter in mammalian cellsDepositorInsertSpCas9 (cas9 Synthetic)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Integrin beta5-2XEGFP
Plasmid#139779PurposeDetecting Integrin beta5 by fluorescence microscopyDepositorInsertIntegrin subunit beta5 (ITGB5 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro-tetON-shRFP
Plasmid#110940PurposeTet-inducible shRNA targeting RFPDepositorInsertshRFP
UseLentiviral and RNAiTagsExpressionMutationPromoterH1/TOAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
SlugCas9
Plasmid#163793PurposeExpresses SlugCas9, and cloning backbone for sgRNADepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
His6tev CBP BCHZ (1079-1751)
Plasmid#99341PurposeBacterial expression of His tagged mouse CBP HAT core Brd-ZZ (1079-1751)DepositorInsertmouse CBP BRD-ZZ domains (1079-1751) (Crebbp Mouse)
UseTagsHis6tevExpressionBacterialMutationPromoterT7Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-hPhyB-mCherry-HRasCT
Plasmid#100281PurposeExpresses Phytochrome B (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cellsDepositorInsertPhytochrome B (PHYB Mustard Weed)
UseTagsmCherry fused with HRas C-terminus for plasma mem…ExpressionMammalianMutationHuman codon optimized PhyB (1-908 aa)PromoterCAG promoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-B7
Plasmid#135509PurposeMammalian expression of myc-tagged HLA-B*07:02DepositorInsertHLA-B*07:02 (HLA-B Human)
UseTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO/GFP-ZNF598
Plasmid#141191PurposeExpresses GFP-ZNF598 in mammalian cells, can be used to make inducible cell lineDepositorInsertZNF598 (ZNF598 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-Intersectin Long
Plasmid#47395DepositorInsertintersectin long (ITSN1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEL031
Plasmid#137900PurposeSTU2.0 SpyCas9 tRNA system for plant genome editingDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)
Plasmid#85006PurposeFor Cre/loxP-based Supernova-GFP, pK038 should be used with pK031. The GFP fragment of pK038 can be replaced with another gene by using SalI and EcoRV sites.DepositorInsertEGFP-ires-tTA
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ Zf ctnna1L344P
Plasmid#185407PurposeZebrafish ctnna1 bearing a point mutation in the vinculin binding site. For in vitro transcription (SP6) or expression through CMVDepositorInsertctnna1 (ctnna1 Zebrafish)
UseIn vitro synthesis of mrnaTagsExpressionMammalianMutationchanged Leucine 344 to ProlinePromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pN1-CMV-L-sDarken
Plasmid#184801PurposeLow affinity version of the Serotonin Sensor sDarken under the control of a CMV Promoter.DepositorInsertL-sDarken
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only