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Plasmid#59812PurposeAllows the integration of GFP hBub1 in the genome and Tet-inducible expression.DepositorInsertBub1 (BUB1 Human)
UseTagsEmGFPExpressionMammalianMutationPromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
p55-H2B-Dendra2
Plasmid#80609PurposeEncodes H2B-Dendra2-fusion protein under the CAG promoter. Linearize using Asc1 and Not1 for mouse embryo injection.DepositorInsertH2B-Dendra2 (H2BC21 Human, Synthetic)
UseTagsDendra2ExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-FNTA-FLAG
Plasmid#86858PurposeTo express FNTA with a FLAG tag at C terminus.DepositorInsertFNTA (FNTA Human)
UseTagsFLAGExpressionMammalianMutationThe sequence of the insert is isoform 2, which di…PromoterCMVAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-GFP-ATL2-deltaTM
Plasmid#109021Purposestable lentiviral expression of cDNADepositorInsertATL2-deltaTM (ATL2 Human)
UseLentiviralTagseGFPExpressionMammalianMutationtruncated form without C-terminal ER transmembran…PromoterEIF-1a promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
3611 pcDNA3 Bim
Plasmid#8804DepositorInsertBim (Bcl2l11 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2006AvailabilityAcademic Institutions and Nonprofits only -
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B9L-BirA*
Plasmid#109146PurposeTo generate stable cell lines expressing B9L-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cellsDepositorInsertB9L (Bcl9l Mouse)
UseTagsBirA(R118G) and FlagExpressionMammalianMutationPromoterCMV/TetO2Available sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro-sgATL2-1
Plasmid#109008PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorInsertATL2-sgRNA #1 (ATL2 Human)
UseLentiviralTagsExpressionMammalianMutationPromotermU6 promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
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3625 pSP72 Bim (genomic)
Plasmid#8805DepositorInsertBim genomic (Bcl2l11 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pDK366
Plasmid#134196PurposeRetroviral plasmid with SKAP insertDepositorInsert5xRD SKAP Alternate Start- LAP (with G5 hardeneded) (KNSTRN Human)
UseRetroviralTagsLAP (GFP)ExpressionMammalianMutation5xRD, Alternate Start, G5 hardenededPromoterAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Atoh1 del3
Plasmid#96913PurposeMurine Atoh1 with amino acids 214-305 removedDepositorInsertAtoh1 (Atoh1 Mouse)
UseTags2xFLAGExpressionMammalianMutationDeletion of amino acids 214-305PromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Atoh1 del4
Plasmid#96914PurposeMurine Atoh1 with amino acids 306-347 removedDepositorInsertAtoh1 (Atoh1 Mouse)
UseTags2xFLAGExpressionMammalianMutationDeletion of amino acids 306-347PromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pCI neo-hEST2
Plasmid#1781DepositorInserthTERT (TERT Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCDH-SIRT2-Flag
Plasmid#102624PurposeExpresses full length SIRT2 isoform 1 in mammalian cellsDepositorInsertSIRT2 (SIRT2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-FIH1
Plasmid#21403DepositorInsertHIF1AN (HIF1AN Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FIH1
Plasmid#21399DepositorInsertHIF1AN (HIF1AN Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-PHD3
Plasmid#21402DepositorInsertEGLN3 (EGLN3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2010AvailabilityAcademic Institutions and Nonprofits only