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Plasmid#124964PurposeExpress human LDLR under human APOAI promoterDepositorInserthuman LDLR (LDLR Human)
UseAdenoviralTagsExpressionMutationPromoterhuman APOAIAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEL063
Plasmid#124895PurposeFor genome editing in plant by SpCas9 variant(SpCas9-NG)DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV.EF1α.mEmerald.CD9.miR9T
Plasmid#170452PurposeLentiviral plasmid that encodes mEmerald-CD9 fusion protein with microRNA 9 tandem cassette to restrict expression to microglia with EF1-alpha promoter.DepositorInsertCD9 (CD9 Human)
UseLentiviralTagsmEmeraldExpressionMutationPromoterEF1aAvailable sinceMay 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBABE-ProDOL
Plasmid#206866PurposeExpression of ProDOL probe - LynAnchor-eGFP-SNAP-tag-HaloTagDepositorArticleInsertProDOL
UseRetroviralTagsHaloTag, His-Tag, SNAP-tag, and eGFPExpressionMammalianMutationContains Lyn amino acids 1-16PromoterAvailable sinceAug. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-mEGFP
Plasmid#185013PurposeBacterial Expression of 6xHis Sirius-GFP Fusion ProteinDepositorInsertSirius-GFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
Flag-neo-PP1-Alpha-H248K
Plasmid#171268PurposeMammalian expression of 3x-Flag-tagged human PP1 alpha catalytic subunit H248K mutant that loses enzymatic activityDepositorInsert3x-Flag-tagged human PP1 alpha catalytic subunit H248K mutant (PPP1CA Human)
UseTagsFlagExpressionMammalianMutationPPP1CA-His248Lys (H248K)PromoterCMVAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
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Dendra2-Vinculin-N-21
Plasmid#57749PurposeLocalization: Focal Adhesions, Excitation: 490 / 553, Emission: 507 / 573DepositorInsertNM_003373.3 (VCL Human)
UseTagsDendra2ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
proISG15 (2-165)
Plasmid#110762PurposeBacterial expression of proISG15DepositorInsertISG15 (ISG15 Human)
UseTagsN-His6-3CExpressionBacterialMutationPromoterT7Available sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-PIF3-mEGFP
Plasmid#100283PurposeExpresses PIF3 (1-100 aa) fused with mEGFP in mammalian cellsDepositorInsertPhytochrome interacting factor 3 (PIF3 Mustard Weed)
UseTagsmEGFPExpressionMammalianMutationN-terminus (1-100 aa) of PIF3 is usedPromoterCAG promoterAvailable sinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
mRFP-human APPL1
Plasmid#22202DepositorInsertAdaptor protein containing PH domain, PTB domain and Leucine zipper motif (APPL1 Human)
UseTagsmRFPExpressionBacterial and MammalianMutationPromoterAvailable sinceOct. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
HA-mDyn3 pcDNA3
Plasmid#36265DepositorInsertDynamin 3 (Dnm3 Mouse)
UseTagsHAExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceApril 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKD001 pME minprom_EGFP
Plasmid#195955PurposeGateway pME fluorophoreDepositorInsertmouse beta-globin minimal promoter EGFP
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA
Plasmid#107270PurposeExpresses Cas9 upon induction with doxicycline.DepositorInsertshSpCas9
rtTA
bGH polyA-SV40polyA
UseTags3xFlag, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationPromoterCAG and TREAvailable sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabe_Zeo_cOVA_T2A_mStrawberry
Plasmid#161736PurposeExpression of cOVA and mStrawberryDepositorInsertcytoplasmic ovalbumin
UseRetroviralTagsmStrawberryExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX317-VRK1
Plasmid#199643PurposeExpresses VRK1DepositorInsertVRK1 (VRK1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-Smad4
Plasmid#89829PurposeKnockdown human Smad4 expression in human mammalian cellsDepositorInsertSmad4 shRNA (SMAD4 Human, Mouse)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-Gluc-RMA-IRES-EGFP
Plasmid#189630PurposeExpresses Gluc-RMA and EGFP in the presence of Cre recombinase. Double-floxed Gluc-RMA and EGFP for monitoring Cre-expressing neuronal populations.DepositorInsertsGaussia luciferase fused to Fc
IRES-EGFP
UseAAV, Cre/Lox, and LuciferaseTagsGluc and IgG1 FcExpressionMammalianMutationPromoterIRES and hSynAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-N-CretrcintG
Plasmid#69570PurposeOne half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for rAAV production and expression in mammalian cellsDepositorHas ServiceAAV1Insertkozak consensus - N-CretrcintG
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-C-CreintG
Plasmid#69571PurposeOne half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for rAAV production and expression in mammalian cellsDepositorHas ServiceAAV1Insertkozak consensus - C-CreintG
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only