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Showing: 9061 - 9080 of 20487 results

  1. pLX_TRC311/NLS-Cas13d-P2A-Blast

    Plasmid
    #212196
    Purpose
    CasRx (NLS-RfxCas13d-NLS) with 2A-Blasticidin for RNA targeting. CasRx-2A-Blasticidin is driven by EF1a promoter. EGFP is driven by SV40
    Depositor
    Stacey Edwards
    Article
    Wang et al Mol Cancer. 2024 May 15;23(1):101. doi:
    Insert
    CasRx (NLS-RfxCas13d-NLS)-HA with 2A-Blasticidin
    Use
    CRISPR and Lentiviral
    Tags
    HA, NLS, and blasticidin S deaminase
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α promoter
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    212196-plasmid-map-sequence-id-420116

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  2. pC0053-CMV-dPspCas13b-GS-ADAR2DD(E488Q)-delta-984-1090

    Plasmid
    #103869
    Purpose
    dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Promoter
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    103869-plasmid-map-sequence-id-199177

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  3. dCas9-p300 HAT

    Plasmid
    #179544
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT domain (aa 1287-1664) driven by AF4 promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human p300 HAT domain (aa 1287-1664) (EP300 Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179544-plasmid-map-sequence-id-354774

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  6. pT3TS-zSpG

    Plasmid
    #179316
    Purpose
    In vitro transcription of SpG-Cas9 mRNA zebrafish codon-optimized from T3 promoter
    Depositor
    Miguel Angel Moreno-Mateos
    Article
    Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:
    Insert
    Zebrafish codon-optimized Cas9 variant SpG
    Use
    CRISPR; In vitro transcription by t3 promoter
    Tags
    SV40-NLS
    Expression
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337R
    Promoter
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    179316-plasmid-map-sequence-id-354720

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  7. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8 (ANV)

    Plasmid
    #71829
    Purpose
    Expression of mutant dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR and specific sgRNA for the human BACH2 promoter; for use as a control
    Depositor
    Vlatka Zoldoš
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    BACH2-sgRNA8 (BACH2 S. pyogenes, Synthetic, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    U6
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71829-plasmid-map-sequence-id-131024

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  9. pT3TS-zSpRY

    Plasmid
    #179317
    Purpose
    In vitro transcription of SpRY-Cas9 mRNA zebrafish codon-optimized from T3 promoter
    Depositor
    Miguel Angel Moreno-Mateos
    Article
    Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:
    Insert
    Zebrafish codon-optimized Cas9 variant SpRY
    Use
    CRISPR; In vitro transcription by t3 promoter
    Tags
    SV40-NLS
    Expression
    Mutation
    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…
    Promoter
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    179317-plasmid-map-sequence-id-354723

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Showing: 9061 - 9080 of 20487 results