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pSCALPS_miR-146aSponge_mCherry_GFP

Plasmid

#149718

Purpose

Lentiviral vector expressing a miR-146a decoy sponge

Depositor

Silvia Monticelli

Article

Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

Insert

14X miR-146a binding sites

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

G to T mutation in position 75 of the insert

Promoter

Available since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX-uORF-HA-birA*-N-Ras(Q61K)-IRES-Puro

Plasmid

#120564

Purpose

Expresses HA-birA*-N-Ras Q61K fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

HA-birA*

Expression

Mammalian

Mutation

Q61K

Promoter

CMV

Available since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pMOS011: superecliptic pHluorin (mitochondrial)

Plasmid

#163050

Purpose

superecliptic pHluorin (mitochondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

superecliptic pHluorin

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pSLIK mCh-V5-NES-EGFP-ERBB2 NLS zeo

Plasmid

#192338

Purpose

Lentiviral expression vector for an inducible mCh-V5-NES-EGFP-ERBB2 NLS

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

mCh-V5-NES-EGFP-ERBB2 NLS (ERBB2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLENTI CMV GFP-TREX1 V235fs BLAST

Plasmid

#164236

Purpose

lentiviral plasmid for GFP-TREX1 V235fs

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

V235 frame shift mutation

Promoter

CMV

Available since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pHR Src deltaSH2/3-TagBFP

Plasmid

#171033

Purpose

TagBFP-tagged Src variant lacking SH2 and SH3 domains.

Depositor

Jared Toettcher

Article

Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

Insert

Src

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pSECC-sgLKB1-A

Plasmid

#138663

Purpose

Expresses a mouse LKB1-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgLKB1 mouse (Stk11 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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RRL.sin.cPPT.SFFV/Ace2.WPRE (MT136)

Plasmid

#145842

Purpose

Lentiviral vector genome to ectopically express human Ace2

Depositor

Caroline Goujon

Article

Rebendenne et al J Virol. 2021 Jan 29. pii: JVI.02415-20.

Insert

human Ace2 (ACE2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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ScFv-2ERT2-V

Plasmid

#120553

Purpose

3rd gen transfer vector. Encode an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP, 2 tandem ERT2 and VP64.

Depositor

Yu Wang

Article

Lu et al Nucleic Acids Res. 2018 Mar 16;46(5):e25

Insert

scFvGCN4, sfGFP, GB1, ERT2, VP64

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hPGK

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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PRKD1 gRNA (BRDN0001146537)

Plasmid

#77228

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKD1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKD1 (PRKD1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLL5.0 hCortKD-Flcortmut3YF EGFP

Plasmid

#187263

Purpose

Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YF mutant

Depositor

Alpha Yap

Article

Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

Insert

Cortactin (CTTN Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

3YF (Y412, Y470, Y486)

Promoter

U6, 5'LTR

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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plentiCRISPRv2-sgPLXNB2

Plasmid

#86152

Purpose

Lentivirus carrying Cas9/CRISPR for cut in human PLXNB2 gene exon 3

Depositor

Roland Friedel

Article

Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

Insert

Plexin-B2 (PLXNB2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-sgRbm10/Cre

Plasmid

#89648

Purpose

Expresses an Rbm10-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Rbm10 (Rbm10 Mouse)

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-GFI1

Plasmid

#139813

Purpose

doxycycline-inducible expression of Human GFI1 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

GFI1 (GFI1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

P2L- Please see depositor comment below

Promoter

TRE-mCMV

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1p shDna2

Plasmid

#31951

Depositor

Sheila Stewart

Article

Duxin et al Mol Cell Biol. 2009 Aug;29(15):4274-82.

Insert

shRNA against hDna2 (DNA2 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pAcBac1-3nitroY-A7-RS

Plasmid

#141173

Purpose

Expression of orthogonal tRNA and tRNA synthetase for incorporation of unnatural amino acids

Depositor

Ryan Mehl

Insert

A7 3nY tRNA synthetase

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

P186T L274R N311G C313N

Promoter

CMV

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-RCM vpx

Plasmid

#115812

Purpose

Encodes codon optimized 3xFLAG-SIVRCM Vpx (AF349680) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVRCM Vpx (AF349680)

Use

Lentiviral

Tags

3x-FLAG

Expression

Mutation

Promoter

SFFV

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-RAF1-N161K

Plasmid

#116656

Purpose

Lentiviral expression of RAF1 N161K

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

RAF1 (RAF1 Human)

Use

Lentiviral

Tags

Expression

Mutation

N161K

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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LeGO-R

Plasmid

#27355

Depositor

Boris Fehse

Article

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

U6 promoter, SFFV promoter, dsRed2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_DNMT3A

Plasmid

#183283

Purpose

All-in-One CRISPRko system with a guide RNA that targets DNMT3A gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

DNMT3A

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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