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Plasmid#176684Purposemembrane localized EphrinA1 RTK fusion to Cry2olig - includes SAM domainDepositorInsertPM-EphA1-Cry2olig-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET29a-human histone 4-in-One
Plasmid#223237PurposePolycistronic bacterial coexpression construct for human His-Sumo-H2A, H2B, H3, and Dual-StrepII-H4DepositorInsertsHis6-VSVG-Histone H2A
Histone H2B
Histone H3
Histone H4-TEV-His6-Dual StrepII
UseTagsDual StrepII, His6, TEV protease site, and VSVGExpressionBacterialMutationA103GPromoterT7Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
MARS
Plasmid#205232PurposeExpresses PLEKHA5 aa 143-271 (K163A and R164A) fused to mScarlet-i in mammalian cellsDepositorInsertPLEKHA5 aa 143-271 with K163A and R164A mutations (PLEKHA5 Human)
UseTagsNuclear Export Sequence and mScarlet-iExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…PromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G-GMIP P363D P364D P366D
Plasmid#221323PurposeDox-inducible expression of GMIP mutant in mammalian cells by retroviral transductionDepositorInsertGMIP (Gmip Mouse)
UseRetroviralTags3xFLAGExpressionMutationP363D P364D P366DPromoterTRE3GAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV HA-RIM2
Plasmid#206151PurposeCMV HA-RIM2DepositorInsertRim2 (Rims2 Rat)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pMSCV-MAVS(pLxIS trunc)
Plasmid#221314PurposeExpression of MAVS truncated pLxIS domain in mammalian cells by retroviral transductionDepositorInsertMAVS truncated pLxIS domain (Mavs Mouse)
UseRetroviralTagsFLAGExpressionMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-MAP2K4
Plasmid#221295PurposeExpression of MAP2K4 (pLxIS) in mammalian cells by retroviral transductionDepositorInsertMAP2K4 (pLxIS) (Map2k4 Mouse)
UseRetroviralTagsFLAG-4xFKBPExpressionMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-GMIP(pLxIS) S31A
Plasmid#221271PurposeExpression of GMIP pLxIS mutant in mammalian cells by retroviral transductionDepositorInsertGMIP pLxIS (Gmip Mouse)
UseRetroviralTags3xFLAG-4xFKBPExpressionMutationS31APromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ChmN-C47A
Plasmid#221367PurposeExpress C47A ChmN in E. coliDepositorInsertchmN-C47A
UseTagsHis6ExpressionBacterialMutationchanged Cys 47 to AlaPromoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC18-GFP
Plasmid#220495PurposeTo generate substrates to monitor Ku overloading and its impact on transcription at the DNA ends vicinity.DepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterSV40Available sinceJuly 3, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLX-EF1a_NKX2-5
Plasmid#221491PurposeNKX2-5 lentiviral overexpressionDepositorInsertNKX2-5 (NKX2-5 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_NKX2-1
Plasmid#221472PurposeGateway cloning of NKX2-1 short isoform overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNoT7
Plasmid#218168PurposepET31b plasmid with short insert lacking T7 and ribosome binding sites as controlDepositorInsertShort sequence, mutated RBS and T7 sites
UseTagsExpressionBacterialMutationPromoterNA - mutated T7Available sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCM4.4
Plasmid#217648Purposean ermE*p-cas9-driven expression, as an alternative plasmidDepositorInsertCodon Optimized SpCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
p104_gRNA_Sp_CD3e
Plasmid#213780PurposeExpresses CRISPRi gRNA for CD3e promoter and GFP. gRNA driven by mU6.DepositorInsertSp gRNA
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
p105_gRNA_Sa_CD40
Plasmid#213781PurposeExpresses CRISPRa gRNA for CD40 promoter and mScarlet. gRNA driven by mU6.DepositorInsertSa gRNA
UseCRISPR and LentiviralTagsExpressionMutationPromotermU6Available sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-1~41-mEGFP(A206K)-TwStrep
Plasmid#208628Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa1~41, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 1~41 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 1~41, other residues not includedPromoterT7Available sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2(ΔmScarlet)
Plasmid#208185PurposeGFP positive, mScarlet-I negative controlDepositorInsertGFPmut2
UseReporterTagsExpressionBacterialMutationPromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
B2401_miRFP-FKBP5x
Plasmid#215118PurposeExpresses miRFP-FKBP-FKBP-FKBP-FKBP-FKBP in mammalian cellsDepositorInsertmiRFP-FKBP-FKBP-FKBP-FKBP-FKBP
UseTagsmiRFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only