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PHAGE-P CMVt N-HA hE6-AP III (AA 1-84)

Plasmid

#119319

Purpose

lentiviral vector for expression of HA-Tagged E6-AP III AA 1-84 in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP III (UBE3A Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

Only AA 1-84

Promoter

CMV

Available since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001145694)

Plasmid

#80191

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pLJM1-FH-AGO2-5xE

Plasmid

#91981

Purpose

Expresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824E, S828E, T830E, S831E, S834E (5xE)

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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PWPXLD-GFP-Numb-WT

Plasmid

#133751

Purpose

Wild-type Numb tagged with GFP cloned into PWPXLD

Depositor

Chay Kuo

Article

Abdi et al Cell Rep. 2019 Aug 20;28(8):2012-2022.e4

Insert

GFP-Numb (Numb Mouse)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

EF1-alpha

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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FLX1.8MitoKate2

Plasmid

#28132

Depositor

Geoffrey Owens

Insert

MitoKate2

Use

Lentiviral

Tags

COX VIII signal sequence

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pCDH_ss-SBP-EGFP

Plasmid

#172358

Purpose

Reporter to monitoring a streptavidin-binding peptide trafficking

Depositor

Guido Kroemer

Article

Zhao et al Sci Rep. 2018 Oct 8;8(1):14966. doi: 10.

Insert

ER importing signal sequence

Use

Lentiviral

Tags

SBP-EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO-PHB-8

Plasmid

#122233

Purpose

Expresses shRNA targeting the coding sequence of human PHB

Depositor

Alex Kentsis

Article

Ortiz et al JCI Insight. 2019 Aug 8;4(15). pii: 1270

Insert

shRNA targeting PHB (see partial depositor seq) (PHB1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pSMPUW_7.1_M2_hcSyn-gateway-V5

Plasmid

#128457

Purpose

lentiviral destination vector with hcSyn promoter and C-terminal V5 tag

Depositor

Lindy Barrett

Insert

hcSyn promoter and C-terminal V5 tag

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

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shRNA GR

Plasmid

#24095

Depositor

Moses Chao

Article

Jeanneteau et al Proc Natl Acad Sci U S A. 2008 Mar 25. 1

Insert

Glucocorticoid Receptor (Nr3c1 Rat)

Use

Lentiviral

Tags

GFP

Expression

Mutation

Promoter

Available since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only
pSLIK-Venus-TmiR-Luc

Plasmid

#25739

Purpose

Control lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA, constitutive Venus fluorescent protein coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Luciferase miR-shRNA

Use

Lentiviral and RNAi

Tags

Venus

Expression

Mammalian

Mutation

Promoter

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pLV.CMV.GFPmiR125sponge

Plasmid

#115974

Purpose

Knockdown of miR-125

Depositor

Johan Jakobsson

Article

Akerblom et al Development. 2014 Apr;141(7):1580-8. doi

Insert

GFPmiR125sponge

Use

Lentiviral

Tags

GFP

Expression

Mutation

Promoter

CMV

Available since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Gata1

Plasmid

#41085

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

Insert

Gata1 (Gata1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TetO + minimal CMV

Available since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only
FUW TetO HA-AMPKa1 AS

Plasmid

#69825

Purpose

Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

minimal CMV promoter with tetracycline operator

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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ERN1 gRNA (BRDN0001149471)

Plasmid

#76410

Purpose

3rd generation lentiviral gRNA plasmid targeting human ERN1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ERN1 (ERN1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLV H2afy-V5

Plasmid

#175122

Purpose

Lentiviral expression of mouse Macroh2a1-V5

Depositor

Larry Gerace

Insert

Macroh2a1 (Macroh2a1 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-BCL6

Plasmid

#139816

Purpose

doxycycline-inducible expression of Human BCL6 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

BCL6 (BCL6 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

T190A, E241G, L314M- please see depositor comments

Promoter

TRE-mCMV

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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SIN-cPPT-H1-sgHTT1-U6-sgGFP2-7SK-sgCas9-PGK-mCherry-WPRE

Plasmid

#87919

Purpose

HIV-1 SIN lentiviral transfer vector

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

sgHTT1, shGFP2, sgCas9, cherry

Use

Lentiviral

Tags

Expression

Mutation

Promoter

H1, U6, 7SK, PGK

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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sgRNA3_IL1RN

Plasmid

#64151

Purpose

Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA3_IL1RN (IL1RN Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pCDH-CB-Nluc-P2A-copGFP-T2A-Puro

Plasmid

#73039

Purpose

Express Nluc, copGFP dual reporter

Depositor

Kazuhiro Oka

Insert

Nluc-P2A-copGFP-T2A-Puro

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

chicken beta-actin + CMV enhancer and chicken bet…

Available since

Jan. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pFIN-EF1a-GFP:2A:CHER(HA)-WPRE

Plasmid

#44597

Depositor

Susan Semple-Rowland

Article

Verrier et al PLoS One. 2011;6(5):e20553. doi: 10.1371

Insert

EF1a-GFP:2A:CHER(HA)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

human elongation factor -1a (pos 2528-3971)

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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