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Plasmid#200261PurposeU6 promoter expressing sgClta. Clta homology arms, sfGFP knock-in.DepositorInsertsfGFP flanked by homology arms targeting Clta, additional U6 promoter expressing sgClta
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro-CAG-fl-STOP-fl-ZEB2-GFP-Flag-WPRE-poly(A)
Plasmid#165456PurposeInducible ZEB2 expression from AAVS1 locusDepositorInsertZEB2 (ZEB2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCAGAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-A2
Plasmid#135504PurposeMammalian expression of myc-tagged HLA-A*02:01DepositorInsertHLA-A*02:01 (HLA-A Human)
UseTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2-STING_gRNA3
Plasmid#127640PurposeKnock-out of human STINGDepositorInsertSTING (STING1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPV-Dual_promoter-EF1α-2xNLS-Cascade+Cas3-P (RD)
Plasmid#204619PurposeExpression vector of Cascade and Cas3. Puromycin selectable.DepositorInsertCas7, Cas5, Cas8, Cas11, Cas6, and Cas3
UseCRISPRTagsEach protein has C- and N-terminal SV40 NLSExpressionMutationPromoterEF1aAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
Opto-CRAC version 1
Plasmid#101245PurposeGenetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signalingDepositorInsertLOV2 and STIM1 cytosolic fragment
UseTagsmCherryExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-E988K
Plasmid#173945PurposeBacterial expression of inactive PARP1 mutant (E988K attenuates catalytic and PAR-branching activity of PARP1)DepositorInsertPARP1-E988K (PARP1 Human)
UseTags6xHisExpressionBacterialMutationV762A, E988KPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-C125G
Plasmid#174792PurposeBacterial expression of a less active PARP1 mutant (C125G reduces affinity for single-strand DNA break)DepositorInsertPARP1-C125G (PARP1 Human)
UseTags6xHisExpressionBacterialMutationC125G, V762APromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1aH N90-beta-catenin
Plasmid#86499PurposeMammalian expression of Myc tagged N90 beta-cateninDepositorInsertbeta-catenin (CTNNB1 Human)
UseTagsMycExpressionMammalianMutationDeletion of amino acids 1-90; constitutively acti…PromoterEF1aAvailable sinceFeb. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1aH-TAZ S89A
Plasmid#179910PurposeConstruct for expressing an activated form of TAZ (encoding be gene WWTR1)DepositorInsertWW Domain Containing Transcription Regulator 1 (WWTR1 Human)
UseTags3X Flag-tagExpressionMammalianMutationSerine 89 mutated to AlaninePromoterEF1aAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PfV-Sapphire-IRES-DSRed
Plasmid#116934PurposeMammalian lenti vector for expression of the 40nm GEM and DSRedDepositorInsertPfv
UseLentiviralTagsSapphireExpressionMutationPromoterCMVAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-egfp-U3-Osp.gRNA
Plasmid#166034PurposeEmpty backbone for expression of gRNA-expressing integration deficient lentiviral particles.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV Gpat4-V5
Plasmid#175145PurposeLentiviral expression of mouse Gpat4-V5DepositorInsertGpat4 (Gpat4 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMCherry F8-2
Plasmid#186034PurposeExpress VHH F8-2 as a mCherry fusion in mammalian cellsDepositorInsertVHH F8-2
UseTagsmCherryExpressionMammalianMutationPromoterCMV immediate earlyAvailable sinceJuly 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-ZFP36
Plasmid#178438Purposedoxycycline-inducible expression of Human ZFP36 in mammalian cellsDepositorInsertZFP36 (ZFP36 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSW648 - pREV>>
Plasmid#115996Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpREV:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailable sinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-EF1Alpha-Tet-on 3G
Plasmid#118592PurposeExpresses Tet-on 3G from the EF1Alpha promoterDepositorInsertTet-on 3G
UseLentiviralTagsExpressionMammalianMutationPromoterEF1AlphaAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS305-Leu2-PINO4-PfV-GS-Sapphire
Plasmid#116930PurposeYeast integrative vector for the exression of the 40nm GEM under the INO4 promoterDepositorInsertPfv
UseTagsSapphireExpressionYeastMutationPromoterINO4Available sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
ACE2
Plasmid#164219PurposeExpresses full length human ACE2 protein, along with TagBFP reporter and Puromycin selection markerDepositorInsertACE2 (ACE2 Human)
UseLentiviralTagsExpressionMutationsilent mutation to remove internal EcoRI site (GA…PromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCV_Mock
Plasmid#223565PurposeThe plasmid is expressed in mammalian cells as a control.DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only