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pCMV-myc-Sema4D RNAiR
Plasmid#51600Purposeexpresses RNAi-resistant mouse Sema4D with N-terminal myc tagDepositorInsertSema4D (Sema4d Mouse)
UseTagsmycExpressionMammalianMutationsilent mutations between amino acids (approx 275-…PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_SBP-EGFP-PLAP
Plasmid#222308PurposeSynchronize the trafficking of PLAP from the ER.DepositorInsertStreptavidin-KDEL and PLAP fused to SBP-EGFP (ALPP Human)
UseTagsExpressionMammalianMutationsignal peptide removedPromoterCMVAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEVOL_PylRS(WT)-tRNA
Plasmid#223512PurposePylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codonDepositorInsertsUseTags6xHisExpressionBacterialMutationPromoteraraBAD, rrnB, and glnS and proKAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-Puro-P2A-NES/PKI-mNeonGreen2x-NLS/FUS
Plasmid#228400PurposeEncodes NES/PKI-mNeonGreen2x-NLS/FUS which is a tandem of mNeongreen with an N-terminal PKI NES and a C-terminal FUS NLS that shuttles between the nucleus and the cytoplasmDepositorInsertNES/PKI-mNeonGreen2x-NLS/FUS
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL4-CMV-NLS/cMyc-AcGFP2x-NES/iKB2-P2A-Puro
Plasmid#228398PurposeEncodes NLS/cMyc-AcGFP2x-NES/iKB2 which is a tandem of AcGFP with an N-terminal cMyc NLS and a C-terminal iKB2 NES that shuttles between the nucleus and the cytoplasmDepositorInsertNLS/cMyc-AcGFP2x-NES/iKB2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pC081: pAAV.CMV.mCherry
Plasmid#205744PurposeAAV vector for expressing CMV-driven mCherry reporter gene.DepositorInsertmCherry
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2puro-E-cadherin(Canis)-exon1 6-28 gRNA
Plasmid#209922PurposeA knock-out vector for the dog CDH1DepositorInsertA gRNA targeting the dog CDH1 gene.
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2puro-p120catenin(Canis)-exon4 116-138 gRNA
Plasmid#209923PurposeA knock-out vector for the dog CTNND1DepositorInsertA gRNA targeting the dog CTNND1 gene.
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCSIIpuro-TGFa-mNeonGreen
Plasmid#209913PurposeTo monitor the expression of TGFα, the plasmid encodes a recombinant TGFα fused intracellularly to the mNeonGreen fluorescent proteinDepositorInsertTGFa-mNeonGreen (TGFA Human)
UseLentiviralTagsmNeonGreenExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE30-DARPin-E11
Plasmid#226784PurposeExpression of His-tagged DARPin E11 in E. coliDepositorInsertDARPin E11
UseTagsFLAG and HisExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLYS6-METTL17-FLAG
Plasmid#220875PurposeExpressing METTL17-FLAGDepositorInsertMETTL17 (METTL17 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG-sgRNA.PRDM1-ZEB2_CRISPRd
Plasmid#216171PurposeExpress the gRNA targeting the PRDM1-binding site in the human ZEB2 locus for the CRISPRd experimentDepositorInsertsgRNA- PRDM1.bs- ZEB2.locus (PRDM1 Human)
UseLentiviralTagsEGFPExpressionMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_Seq17_VS33_4.26_ChimeraIntron_3NLS_RFP_WPRE_polyA
Plasmid#220225PurposeAAV-STARR-seq validation vector containing VS33 enhancerDepositorInsertEnhancer (VS33)
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-GFP-WT1 +KTS N terminal HA
Plasmid#217639PurposeEntry vector with WT1 +KTS mutant with N terminal HA for gateway cloningDepositorInsertWT1 +KTS isoform (WT1 Human)
UseGateway cloningTagsHAExpressionMutationPromoterAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-GFP-WT1 +KTS C terminal HA
Plasmid#217640PurposeEntry vector with WT1 +KTS mutant with C terminal HA for gateway cloningDepositorInsertWT1 +KTS isoform (WT1 Human)
UseGateway cloningTagsHAExpressionMutationPromoterAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-GFP-WT1 -KTS N terminal HA
Plasmid#217641PurposeEntry vector with WT1 -KTS mutant with N terminal HA for gateway cloningDepositorInsertWT1 -KTS isoform (WT1 Human)
UseGateway cloningTagsHAExpressionMutationp.408_410delKTSPromoterAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-GFP-WT1 -KTS C terminal HA
Plasmid#217642PurposeEntry vector with WT1 -KTS mutant with C terminal HA for gateway cloningDepositorInsertWT1 -KTS isoform (WT1 Human)
UseGateway cloningTagsHAExpressionMutationp.408_410delKTSPromoterAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJH4562
Plasmid#200780PurposePflp-18 LoxP EBFP LoxP GtACR2 mCherry unc-54 3' UTR C.elegans AVA and other neurons expression of GtACR2 RFPDepositorInsertGtAR2
UseTagsmCherryExpressionWormMutationPromoterPflp-18Available sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJH4609
Plasmid#200781PurposePpdf-1 LoxP EBFP LoxP GtACR2 mCherry unc-54 3' UTR C.elegans AVB and other neurons expression of GtACR2 RFPDepositorInsertGtACR2
UseTagsmCherryExpressionWormMutationPromoterPpdf-1Available sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only
