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Plasmid#128098PurposeCRISPR gRNA against human TSC2 with Cas9 from S. pyogenes and 2A-EGFPDepositorInsertsgRNA against human TSC2 (TSC2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL235 NheI-Cas9-KpnI-GSAlinker-FseI-TdT-NotI
Plasmid#126480PurposeExpresses Cas9-GSAlinker-TdT in mammalian cells.DepositorInsertsUseTagsExpressionMammalianMutationChanged codons to reduce repetitive sequences.PromoterCMVAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/HIS/FLAG/HA-ALKBH5
Plasmid#38073DepositorInsertALKBH5 alkB, alkylation repair homolog 5 (E. coli) (ALKBH5 Human)
UseTagsHIS/FLAG/HAExpressionMammalianMutationcDNA clone MGC:71143 IMAGE:6339184 with longer 3&…PromoterAvailable sinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO (pEHA1339)
Plasmid#209088PurposeBackbone plasmid for Tet Responsive transgene expression, FRT integrationDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP185 pLVP-dCas9-DNMT3a V2
Plasmid#100936PurposedCas9 and DNMT WT on C terminus, 4 NLS, driven by pGK promoter, with P2A site and PURO gene, within a lentiviral transfer backboneDepositorInsertdCas9, DNMT3a catalytic domain (DNMT3A S.pyogenes, Human)
UseCRISPR and LentiviralTags3xHA and 3xTy1ExpressionMammalianMutationD10A, H840A for dCas9PromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP mScarlet-MOSPD2 W201E
Plasmid#186477PurposeExpression of human MOSPD2 (mutant W201E, unable to bind lipid droplets) fused to mScarlet in mammalian cellsDepositorInsertMOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
UseRetroviralTagsmScarletExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2GG
Plasmid#203756PurposeEncodes a modified version of the membrane anchor of KRas, including the last 16 residues of the C terminus, with mEos3.2 fused to the N terminus. The base pairs encoding the CAAX box for KRas were replaced by the CAAX box from rap1B, resulting in a geranylgeranylation instead of farnesylation. Used as a fluorescent plasma membrane probe.DepositorInsertKRas c-term (KRAS Human)
UseTagsmEos3.2ExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBabe-GFP-IKBalpha-wt
Plasmid#15263DepositorInsertIKB alpha (NFKBIA Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
PX458-3xHA-en31FnCas9ABEmax8.17d
Plasmid#201956PurposeMammalian expression plasmid of en31FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNADepositorInsertbpNLS-en31FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
UseCRISPRTags3xHA, NLS, and T2A-EGFPExpressionMammalianMutationPromoterCbhAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/HIS/FLAG/HA-HNRNPU
Plasmid#38068DepositorInsertHNRNPU heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A) (HNRNPU Human)
UseTagsHIS/FLAG/HAExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
Lenti-117G-hyA3A-BE4max
Plasmid#157945PurposeLentiviral expression of hyA3A-BE4maxDepositorInsertLenti-117G- hyA3A-BE4max
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3 ss-3xFLAG-hLRP5 (aa 32-1615)
Plasmid#115788PurposeExpresses human LRP5 with N-terminal 3xflag tag in mammalian cells, contains artificial signal sequence upstream of tag.DepositorInsertLRP5 (LRP5 Human)
UseTags3xflagExpressionMammalianMutationcontains silent mutation that creates an internal…PromoterCMVAvailable sinceOct. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOTTC374 - pAAV EF1a DIO iRFP
Plasmid#47626PurposeAn AAV vector that expresses iRFP in a Cre-dependent manner (DIO, double floxed inverted open reading frame).DepositorInsertInfrared fluorescent protein
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBabe-GFP-IKBalpha-mut (super repressor)
Plasmid#15264DepositorInsertIKB alpha (NFKBIA Human)
UseRetroviralTagsExpressionMammalianMutationSerine 32 to Alanine, Serine 36 to AlaninePromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag MKK7B2Jnk2a2
Plasmid#19727DepositorUseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2009AvailabilityAcademic Institutions and Nonprofits only -
A&C-BEmax
Plasmid#157947PurposeMammalian expression of A&C-BEmaxDepositorInsertA&C-BEmax
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PPO-Venus
Plasmid#139502PurposeExpresses Venus-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.DepositorInsertparapinopsin
UseTagsVenus tag following 3x Ala linkerExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/HIS/FLAG/HA-HNRNPD
Plasmid#38066DepositorInsertHNRNPD heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa) (HNRNPD Human)
UseTagsHIS/FLAG/HAExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
AID-BE4max-P2A-GFP
Plasmid#157948PurposeMammalian expression of AID-BE4max-P2A-GFPDepositorInsertAID-BE4max
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PPO-mScarlet
Plasmid#139503PurposeExpresses mScarlet-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.DepositorInsertparapinopsin
UseTagsmScarlet following Gly-Gly-Ser linkerExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only