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Showing: 881 - 900 of 6476 results

  1. pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Amber

    Plasmid
    #105806
    Purpose
    Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Amber, cleavable by TEV. Control for protein interaction analysis by FRET.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    ORF1: 3C-Cerulean; ORF2: TEV-Amber
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    105806-plasmid-map-sequence-id-207907

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  2. pZC07 (pFA6a-TEV-6xGly-PrtA-HphMX)

    Plasmid
    #44084
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, HphM…
    Expression
    Mutation
    Promoter
    Available since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44084-plasmid-map-sequence-id-61690

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  3. pACEBac1-PSMB5-PSMB6-PSMB7-TEV-2xSTII

    Plasmid
    #217896
    Purpose
    Insect cell expression of human 20S CP proteasome subunit beta type-5 (PSMB5), beta type-6 (PSMB6), and beta type-7 (PSMB7) with C-terminal Twin-Strep-tag
    Depositor
    Brenda Schulman
    Article
    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1
    Inserts
    PSMB5 (PSMB5 Human)
    PSMB6 (PSMB6 Human)
    PSMB7 (PSMB7 Human)
    Use
    Tags
    2xSTII
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    217896-plasmid-map-sequence-id-444196

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  4. pFastBac HTB His-TEV-rDPP9-FLAG

    Plasmid
    #217328
    Purpose
    Purification of rat DPP9 fusion with C-terminal FLAG tag from insect cells
    Depositor
    Yifan Wang
    Insert
    DPP9
    Use
    Tags
    FLAG and His-TEV
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217328-plasmid-map-sequence-id-433437

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  5. pFastBac HTB His-TEV-rDPP9(S729A)

    Plasmid
    #217327
    Purpose
    Purification of catalytically-dead rat DPP9 from insect cells
    Depositor
    Yifan Wang
    Insert
    DPP9
    Use
    Tags
    His-TEV
    Expression
    Insect
    Mutation
    S729A
    Promoter
    polyhedrin
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217327-plasmid-map-sequence-id-433438

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  6. pFastBac HTB His-TEV-BN-rNLRP1-FLAG

    Plasmid
    #217323
    Purpose
    Purification of Brown Norway rat NLRP1 fusion with C-terminal FLAG tag from insect cells
    Depositor
    Yifan Wang
    Insert
    NLRP1
    Use
    Tags
    FLAG and His-TEV
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217323-plasmid-map-sequence-id-438442

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  10. DRD2-V2tail-TevN-BLITz-TetR-FKBP

    Plasmid
    #210514
    Purpose
    expresses DRD2-V2tail-TevN-BLITz-TetR-FKBP component in mammalian cells
    Depositor
    Dongmin Lee
    Insert
    DRD2-V2tail-TevN-BLITz-TetR-FKBP
    Use
    Tags
    HA signal FALG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    210514-plasmid-map-sequence-id-415568

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  11. pET-Duet-6xHis-TEV-Nsp8(76-198)

    Plasmid
    #201024
    Purpose
    Bacterial expression of codon optimized SARS-CoV-2 nsp8 residues 76-198, with an N-terminus His tag followed by a TEV cleavage site.
    Depositor
    Sharon Rozovsky
    Article
    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d
    Insert
    non-structural protein 8 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available since
    May 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    201024-plasmid-map-sequence-id-394447

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  12. His SUMO TEV R354H SPOP 28-359

    Plasmid
    #197011
    Purpose
    E. coli expression vector
    Depositor
    Tanja Mittag
    Insert
    SPOP (Spop Human)
    Use
    Tags
    6His-SUMO-TEV
    Expression
    Bacterial
    Mutation
    R354H
    Promoter
    Available since
    May 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    197011-plasmid-map-sequence-id-392954

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  14. His SUMO TEV W131G SPOP 1-374

    Plasmid
    #197019
    Purpose
    E. coli expression vector
    Depositor
    Tanja Mittag
    Insert
    SPOP (Spop Mouse)
    Use
    Tags
    6His-SUMO-TEV
    Expression
    Bacterial
    Mutation
    W131G
    Promoter
    Available since
    April 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    197019-plasmid-map-sequence-id-390432

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  15. His SUMO TEV E78K SPOP 1-374

    Plasmid
    #197018
    Purpose
    E. coli expression vector
    Depositor
    Tanja Mittag
    Insert
    SPOP (Spop Mouse)
    Use
    Tags
    6His-SUMO-TEV
    Expression
    Bacterial
    Mutation
    E78K
    Promoter
    Available since
    April 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    197018-plasmid-map-sequence-id-389539

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  16. His SUMO TEV E47K SPOP 1-374

    Plasmid
    #197017
    Purpose
    E. coli expression vector
    Depositor
    Tanja Mittag
    Insert
    SPOP (Spop Mouse)
    Use
    Tags
    6His-SUMO-TEV
    Expression
    Bacterial
    Mutation
    E47K
    Promoter
    Available since
    April 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    197017-plasmid-map-sequence-id-389538

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  17. His SUMO TEV W22R SPOP 1-374

    Plasmid
    #197016
    Purpose
    E. coli expression vector
    Depositor
    Tanja Mittag
    Insert
    SPOP (Spop Mouse)
    Use
    Tags
    6His-SUMO-TEV
    Expression
    Bacterial
    Mutation
    W22R
    Promoter
    Available since
    April 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    197016-plasmid-map-sequence-id-389536

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Showing: 881 - 900 of 6476 results