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Pdgfra-T2A-H2B-eGFP donor

Plasmid

#113120

Purpose

Donor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Pdgfra coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Pdgfra donor region including homology arms and T2A-H2B-eGFP (Pdgfra Mouse, Synthetic)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pTol2 hsp70l loxP-Non-FP-loxP-nCas9n-GFP

Plasmid

#158963

Purpose

Cre-dependet Cas9-GFP expression

Depositor

Michael Brand

Article

Kesavan et al Development. 2020 Jun 8;147(11). pii: de

Inserts

hsp70l promoter
floxed Non-FP cassette
Cas9-GFP

Use

CRISPR and Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pBT346_pLambda-attB-pCA-GFP-pA

Plasmid

#52551

Purpose

for producing PhiC31 attB-containing minicircle in vitro

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

Lambda integrase attL1
Lambda integrase attR1
PhiC31o attB (full length)
GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pMK278 (mClover-Hygro)

Plasmid

#72794

Purpose

C-terminal tagging with mClover using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mClover-Hygro

Use

CRISPR

Tags

mClover

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pY2F

Plasmid

#53285

Purpose

Contains an Arabidopsis thaliana lcyE cDNA and produces an active lycopene epsilon-cyclase enzyme in E. coli. Use with pAC-BETA-At or pAC-BETAipi-At to produce alpha-carotene in E. coli.

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

Insert

lcyE (LUT2 Mustard Weed)

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

April 16, 2015

Availability

Academic Institutions and Nonprofits only

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pMK364 (CMV-OsTIR1-loxP-PURO-loxP)

Plasmid

#121184

Purpose

OsTIR1 introduction

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

CMV-OsTIR1-loxP-PURO-loxP

Use

CRISPR

Tags

Expression

Mutation

OsTIR1 codon optimized for human cells

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pACT5FVMF(f)

Plasmid

#110549

Purpose

Control plasmid for titering FV by qPCR to determine the genome-containing particle number. This qPCR protocol is described in Hendrie et al., 2008

Depositor

David Russell

Article

Hendrie et al Mol Ther. 2008 Mar;16(3):534-40. doi: 10

Insert

EGFP, FV LTRs

Use

Qpcr control for titering foamy virus

Tags

Expression

Mutation

Promoter

promoter: MLV LTR

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAID1.2-EF1a-NGFP-mAID

Plasmid

#140607

Purpose

Expression of OsTIR1 and GFP-mAID protein in mammalian cells under the control of EF1a promoter

Depositor

Tatsuo Fukagawa

Article

Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

Insert

OsTIR1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pGWKS7

Plasmid

#139414

Purpose

Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mRuby3 marker.

Depositor

James Fraser

Article

Spencer et al Fungal Genet Biol. 2020 May;138:103365.

Insert

mRuby3

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pBS AluYa5rescueA70D

Plasmid

#78657

Purpose

Generates Alu inserts suitable for rescue

Depositor

Astrid Roy-Engel

Article

Wagstaff et al PLoS Genet. 2012;8(8):e1002842. doi: 10.

Insert

Ya5rescue cassette

Use

Tags

Expression

Mutation

Promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pH6Z-806

Plasmid

#85255

Purpose

Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

Depositor

Philip Pellett

Article

Lindquester et al Arch Virol. 1996;141(2):367-79.

Insert

fragment of the human herpesvirus 6B strain Z29 genome

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pSAG1::CAS9-U6::sg290860-6

Plasmid

#59855

Purpose

Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for CRISPR disruption of TGME49_290860.

Depositor

David Sibley

Article

Behnke et al Eukaryot Cell. 2014 Dec 5. pii: EC.00229

Insert

CRISPR sg290860-6

Use

CRISPR; ; toxoplasma gondii

Tags

Expression

Mutation

Promoter

Available since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pFT1.0

Plasmid

#46138

Depositor

Wenbiao Chen

Article

Ni et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

mCherry

Use

Cre/Lox; Mutagenic gene trap for zebrafish

Tags

Expression

Mutation

Promoter

Available since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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pMK282 (mCherry2-Bsr)

Plasmid

#72798

Purpose

C-terminal tagging with mCherry2 using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mCherry2-Bsr

Use

CRISPR

Tags

mCherry2

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pGWKS10

Plasmid

#139417

Purpose

Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Clover marker.

Depositor

James Fraser

Article

Spencer et al Fungal Genet Biol. 2020 May;138:103365.

Insert

Clover

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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GLI-3 bs-2

Plasmid

#16420

Depositor

Bert Vogelstein

Article

Kinzler et al Nature. 1988 Mar 24. 332(6162):371-4.

Insert

GLI3 (GLI3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 10, 2008

Availability

Academic Institutions and Nonprofits only
pMK279 (mClover-Bsr)

Plasmid

#72795

Purpose

C-terminal tagging with mClover using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mClover-Bsr

Use

CRISPR

Tags

mClover

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pBS hTSP-5

Plasmid

#13002

Depositor

Jack Lawler

Article

Newton et al Genomics. 1994 Dec . 24(3):435-9.

Insert

COMP (COMP Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2006

Availability

Academic Institutions and Nonprofits only
pBSKΔB

Plasmid

#177268

Purpose

Backbone vector for STREAMING-tag knockin vector

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pDctp-mSox10-Mp1

Plasmid

#13513

Depositor

William Pavan

Article

Hakami et al Mech Dev. 2006 Feb . 123(2):124-34.

Insert

Sox10 (Sox10 Mouse)

Use

Tags

Dct promoter and Mp1-polyA

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only

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