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Plasmid#91022PurposeModule A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSPDepositorInsertCsy4-P2A-AtCas9_dead (D10A + H840A)
UseCRISPRTagsExpressionMutationD10A, H840APromoter35SAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A1610
Plasmid#91038PurposeModule A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_D10A nickase, Terminator: HSPDepositorInsertCsy4-P2A-TaCas9_D10A nickase
UseCRISPRTagsExpressionMutationD10APromoterZmUbiAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTaCas9_6
Plasmid#91169PurposeCas9 only expression, Cas9 Type: TaCas9, Plant Selection: PvUbi2:barDepositorInsertTaCas9
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_10A
Plasmid#91207PurposeDirect Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: noneDepositorInsert35S:AtCas9 + AtU6:gRNA
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A1810
Plasmid#91040PurposeModule A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_dead (D10A + H840A), Terminator: HSPDepositorInsertCsy4-P2A-TaCas9_dead (D10A + H840A)
UseCRISPRTagsExpressionMutationD10A, H840APromoterZmUbiAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYZ_3WJrep_N11_trigger
Plasmid#132733PurposeT7 RNAP-driven expression of 3WJ repressor index 11 trigger RNADepositorInsert3WJ_N11_trigger
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A1710
Plasmid#91039PurposeModule A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_H840A nickase, Terminator: HSPDepositorInsertCsy4-P2A-TaCas9_H840A nickase
UseCRISPRTagsExpressionMutationH840APromoterZmUbiAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTC364
Plasmid#91213Purposeprotoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, Csy4 processing)DepositorInsert2 gRNAs targeting tomato ARF8A
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTaCas9-D10A_5
Plasmid#91170PurposeCas9 only expression, Cas9 Type: TaCas9_D10A (nickase), Plant Selection: PvUbi2:hpt IIDepositorInsertTaCas9_D10A (nickase)
UseCRISPRTagsExpressionPlantMutationD10APromoterAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0608
Plasmid#91018PurposeModule A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_D10A nickase, Terminator: HSPDepositorInsertCsy4-P2A-AtCas9_D10A nickase
UseCRISPRTagsExpressionMutationD10APromoterEC1.2Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CAG-sbGLuc-hGtARC2-EYFP
Plasmid#114111Purposefusion protein of Gaussia luciferase variant slow burn, Guillardia theta anion channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertsGaussia luciferase, slow burn
Guillardia theta anion channelrhodopsin-2, human codon optimized
Enhanced Yellow Fluorescent Protein
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTaCas9-H840A_6
Plasmid#91173PurposeCas9 only expression, Cas9 Type: TaCas9_H840A (nickase), Plant Selection: PvUbi2:barDepositorInsertTaCas9_H840A (nickase)
UseCRISPRTagsExpressionPlantMutationH840APromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT/TO Plk4 KD ∆24-mCherry
Plasmid#80272Purposemammalian expression plasmid for kinase dead Plk4 with multi-phospho degron deletedDepositorInsertPlk4 (PLK4 Human)
UseTagsmCherryExpressionMammalianMutationdeletion of multi-phospho degron (amino acids 282…PromoterCMVAvailable sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAtCas9-H840A_1
Plasmid#91162PurposeCas9 only expression, Cas9 Type: AtCas9_H840A (nickase), Plant Selection: 2x35S:hpt IIDepositorInsertAtCas9_H840A (nickase)
UseCRISPRTagsExpressionPlantMutationH840APromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0802
Plasmid#91023PurposeModule A, Promoter: AtUbi10, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSPDepositorInsertCsy4-P2A-AtCas9_dead (D10A + H840A)
UseCRISPRTagsExpressionMutationD10A, H840APromoterAtUbi10Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJG493
Plasmid#91197PurposeWDV replicon T-DNA for gene targeting in wheat scutella, no Cas9 control (gUbi1+gUbi8+donor)DepositorInsertgUbi1+gUbi8+donor
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJIT163-AFID-3
Plasmid#167359PurposeFor inducing predictable deletions in protoplasts and wheat plantsDepositorInsertA3A-Cas9-UDG-T2A-AP lyase
UseTagsExpressionPlantMutationWTPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTaCas9_5
Plasmid#91168PurposeCas9 only expression, Cas9 Type: TaCas9, Plant Selection: PvUbi2:hpt IIDepositorInsertTaCas9
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAtCas9-H840A_2
Plasmid#91163PurposeCas9 only expression, Cas9 Type: AtCas9_H840A (nickase), Plant Selection: 2x35S:npt IIDepositorInsertAtCas9_H840A (nickase)
UseCRISPRTagsExpressionPlantMutationH840APromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTC393
Plasmid#91221Purposeprotoplast vector expressing gRNA24 targeting tomato ANT1 (with TREX2 expression)DepositorInsertgRNA targeting tomato ANT1
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only