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Plasmid#194990Purposepromoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P3 position (I/J)DepositorInsert(I) pGPD (J)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(S) insD_ku70 (Q) (SBE139)
Plasmid#187627Purposethe insertional region downstream for the deletion of the KU70 gene in R. toruloides. Overhangs S/QDepositorInsert(S) insD KU70 (Q)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(P) insUP_ku70 (SBE138) (B)
Plasmid#187626Purposethe insertional region upstream for the deletion of the KU70 gene in R. toruloides. Overhangs P/BDepositorInsert(P) insUP KU70 (B)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAV10.K3.loxP
Plasmid#63202PurposepCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAV10.K5.loxP
Plasmid#63204PurposepCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAV10.HO3.loxP
Plasmid#63205PurposepCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAV10.HO6
Plasmid#63211PurposepCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into HO locus (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pHIE096 CMV Bxb1
Plasmid#139252PurposeEncodes a Bxb1 recombinase for Landing Pad integration.DepositorInsertBxb1 Recombinase
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD1019 TRE3GV DsRedExpress2 in TU2
Plasmid#139254PurposeTRE3GV promoter and DsRedExpress2 in TUPV2DepositorInsertDsRedExpress2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GVAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM467 TRE3GV EYFP in TU3
Plasmid#139255PurposeTRE3GV promoter and EYFP in TUPV3DepositorInsertEYFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GVAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
PT7-alphaHL
Plasmid#227650PurposepT7-driven expression of alpha-hemolysin, a pore-forming protein in lipid bilayers.DepositorInsertalpha-hemolysin
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBD006
Plasmid#201306PurposeD16: Promoter of TraesCS5D02G320700 as Golden Gate compatible MoClo PROM partDepositorInsertD16: Promoter of TraesCS5D02G320700
UseTagsExpressionPlantMutationremoval of BsaI and BpiI sitesPromoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBD004
Plasmid#201304PurposeD14: Promoter of TraesCS3D02G368800 as Golden Gate compatible MoClo PROM partDepositorInsertD14: Promoter of TraesCS3D02G368800
UseTagsExpressionPlantMutationremoval of BsaI and BpiI sitesPromoterAvailable sinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBD005
Plasmid#201305PurposeD15: Promoter of TraesCS3D02G517500 as Golden Gate compatible MoClo PROM partDepositorInsertD15: Promoter of TraesCS3D02G517500
UseTagsExpressionPlantMutationremoval of BsaI and BpiI sitesPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPD1023 TRE3GV DsRedExpress2 in TU5
Plasmid#139257PurposeTRE3GV promoter and DsRedExpress2 in TUPV5DepositorInsertDsRedExpress2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GVAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
C77m
Plasmid#120989PurposeMoClo golden gate assembly CD partfor AmpR (ampicillin/carbenicillin resistance protein). Please see Supplemental Documents for annotated Genbank file.DepositorInsertAmpR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C78m
Plasmid#120990PurposeMoClo golden gate assembly CD cloning part for ChlorR (chloramphenicol resistance protein). Please see Supplemental Documents for annotated Genbank file.DepositorInsertChlorR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C80m
Plasmid#120992PurposeMoClo golden gate assembly CD part for mfLon protease (see doi: [10.1038/nbt.3053]). Please see Supplemental Documents for annotated Genbank file.DepositorInsertmfLon protease
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICSL11085_GG_Wheat_live_Cas9_for
Plasmid#126881PurposeEncodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids.DepositorInsertWheat_live_Cas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
C86m
Plasmid#120997PurposeMoClo golden gate assembly CD part (phage lysis protein; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.DepositorInsertX174E
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only