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Plasmid#164036PurposeImproved PhoCl variant with faster dissociation rateDepositorInsertPhoCl2f
UseTagsHis tag, T7 tag, Xpress TagExpressionBacterialMutationPromoteraraBad promotorAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-Muc2D1D2D3cysD1
Plasmid#155214PurposeExpresses human MUC2 residues 21-1397DepositorInsertMuc2 D1D2D3cysD1 (MUC2 Human)
UseTags6xHistadineExpressionMutationPromoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM6-mCherry
Plasmid#66534PurposeExpresses mCherry under the control of the Concensus T7 PromoterDepositorInsertmCherry
UseSynthetic BiologyTagsExpressionBacterialMutationCodon Optimized for E. coliPromoterConsensus T7Available sinceJuly 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETM6-P.accABCD
Plasmid#66866PurposeEncodes accA, accB, accC, accD in Pseudo-operon configuration.DepositorInsertsaccA
accB
accC
accD
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCoofy63
Plasmid#122015PurposeBaculovirus vector for parallel SLIC cloning containing N-terminal His6-Smt3Star (SumoStar*) and different C-tag optionsDepositorTypeEmpty backboneUseTagsHis6-Smt3Star and Multi3: depending on the LP2 pr…ExpressionInsectMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCoofy41
Plasmid#55184PurposeInsect expression vector for parallel SLIC cloning. C-terminal tags can be added during SLIC.DepositorTypeEmpty backboneUseTagsHis10-Stop-OneStrep-Stop-S-tag-Stop-CBP-HPC4-Stop…ExpressionBacterialMutationPromoterpPH (Polyhedrin promoter)Available sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCoofy64
Plasmid#122016PurposeBaculovirus vector for parallel SLIC cloning containing gp67 signal sequence, N-terminal His6-Smt3Star (SumoStar*) and different C-tag optionsDepositorTypeEmpty backboneUseTagsHis6-Smt3Star and Multi3: depending on the LP2 pr…ExpressionInsectMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
FXN gRNA (BRDN0001144784)
Plasmid#77813Purpose3rd generation lentiviral gRNA plasmid targeting human FXNDepositorInsertFXN (FXN Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
FXN gRNA (BRDN0001145648)
Plasmid#77814Purpose3rd generation lentiviral gRNA plasmid targeting human FXNDepositorInsertFXN (FXN Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
FXN gRNA (BRDN0001146360)
Plasmid#77815Purpose3rd generation lentiviral gRNA plasmid targeting human FXNDepositorInsertFXN (FXN Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-eGFP
Plasmid#36083Purpose3rd generation lentiviral transfer plasmidDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJune 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRU294
Plasmid#167689PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::Cas9i
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRU295
Plasmid#167694PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::Cas9i
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRU324
Plasmid#167691PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::Cas9i
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM6-C4-mCherry
Plasmid#66530PurposeExpresses mCherry under the control of Mutant T7 Promoter C4DepositorInsertmCherry
UseSynthetic BiologyTagsExpressionBacterialMutationCodon Optimized for E. coliPromoterMutant T7 Promoter - C4Available sinceJuly 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UbC-mCherry Rab5
Plasmid#115239PurposeTo express in mammalian cells the endocytic protein Rab5 fused to mCherry by transfection or by generating AAV virions.DepositorInsertRab5a (RAB5A Human, Homo sapiens)
UseAAVTagsmCherryExpressionMammalianMutationPromoterHuman UbCAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
S3_001_026_MV2
Plasmid#134422PurposepYTKMV2-pTDH3_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
NLS_FdeR-N
UseTagsnoneExpressionYeastMutationnonePromoterpGMP1 and pTDH3Available sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM6-HpaBC
Plasmid#71260PurposeExpresses E. coli HpaBC in monocistronic form from ePathBrick vector, pETM6DepositorInsertsHpaB
HpaC
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCoofy44
Plasmid#55185PurposeInsect expression vector for parallel SLIC cloning containing N-terminal 10x His and SumoStar tags. C-terminal tags can be added during SLIC.DepositorTypeEmpty backboneUseTags10x His, 10x His-Stop-OneStrep-Stop-S-tag-Stop-HP…ExpressionBacterialMutationPromoterpPH (Polyhedrin promoter)Available sinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFGAMS-mCherry
Plasmid#166024PurposeExpresses human PFAS-mCherry fusion in mammalian cellsDepositorInsertPFAS-mCherry (PFAS Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only