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Showing: 881 - 900 of 4747 results

  6. pCMV-T7-xCas9(3.7)-P2A-EGFP (RTW3576)

    Plasmid
    #139993
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized xCas9(3.7)(A262T/R324L/S409I/E480K/E543D/M694I/E1219V) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized xCas9(3.7) with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    xCas9(3.7)=A262T/R324L/S409I/E480K/E543D/M694I/E1…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139993-plasmid-map-sequence-id-275508

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  7. pUC19-E3CY56

    Plasmid
    #103075
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    E3CY56(Cas9 coding gene from Aminomonas paucivorans DSM 12260)
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103075-plasmid-map-sequence-id-201714

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  8. pCMV-T7-ABEmax(7.10)-xCas9(3.7)-P2A-EGFP (RTW4644)

    Plasmid
    #140004
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized ABEmax(7.10) xCas9(3.7) with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140004-plasmid-map-sequence-id-275398

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  9. pcDNA5-FRT-TO-Nsp13-HF_GC3opt

    Plasmid
    #157716
    Purpose
    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    Nsp13-HF_GC3opt (ORF1ab SARS-CoV-2)
    Use
    Tags
    His8-Flag
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Promoter
    Available since
    Aug. 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157716-plasmid-map-sequence-id-307912

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  10. pcDNA5-FRT-TO-FH-Nsp13_GC3opt

    Plasmid
    #157715
    Purpose
    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    FH-Nsp13_GC3opt (ORF1ab SARS-CoV-2)
    Use
    Tags
    Flag-His8
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Promoter
    Available since
    Aug. 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157715-plasmid-map-sequence-id-307906

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  15. pTET-IUPi

    Plasmid
    #122019
    Purpose
    Expresses IUP pathway genes (ChK, IPK, idi) under inducible promoter. aTC inducible. Kan. resist.
    Depositor
    Gregory Stephanopoulos
    Article
    Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p
    Inserts
    Choline kinase (CKI1 Budding Yeast)
    Isopentenyl monophosphate kinase (AT1G26640 Mustard Weed)
    isopentenyl-diphosphate Delta-isomerase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Codon optimized for E. coli
    Promoter
    pTET
    Available since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    122019-plasmid-map-sequence-id-238972

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  16. pCMV-T7-hAcrVA2.1-NLS(sv40) (BPK5059)

    Plasmid
    #115137
    Purpose
    CMV-T7 promoter expression plasmid for human codon optimized AcrVA2.1 anti-CRISPR protein with C-terminal NLS
    Depositor
    Benjamin Kleinstiver, Keith Joung, Joseph Bondy-Denomy
    Article
    Marino et al Science. 2018 Sep 6. pii: science.aau517
    Insert
    human codon optimized AcrVA2.1 anti-CRISPR protein
    Use
    Tags
    NLS(SV40)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    115137-plasmid-map-sequence-id-220492

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  17. pCMV-T7-hAcrVA1-NLS(sv40) (BPK5050)

    Plasmid
    #115136
    Purpose
    CMV-T7 promoter expression plasmid for human codon optimized AcrVA1 anti-CRISPR protein with C-terminal NLS
    Depositor
    Benjamin Kleinstiver, Keith Joung, Joseph Bondy-Denomy
    Article
    Marino et al Science. 2018 Sep 6. pii: science.aau517
    Insert
    human codon optimized AcrVA1 anti-CRISPR protein
    Use
    Tags
    NLS(SV40)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    115136-plasmid-map-sequence-id-220499

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  18. pcDNA-FRT-TO-Nsp7-Nsp8_GC3opt

    Plasmid
    #157691
    Purpose
    mammalian expression of untagged SARS-CoV-2 Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    Nsp7-Nsp8_GC3opt (ORF1ab SARS-CoV-2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Promoter
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157691-plasmid-map-sequence-id-307833

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  19. pUC19-G2ZYP2

    Plasmid
    #103110
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    G2ZYP2(Cas9 coding gene from Ralstonia syzygii R24)
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103110-plasmid-map-sequence-id-201819

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  20. pCAG-hMb3Cas12a-NLS(nucleoplasmin)-3xHA (RTW2500)

    Plasmid
    #115142
    Purpose
    CAG promoter expression plasmid for human codon optimized Mb3Cas12a nuclease with C-terminal NLS and HA tag
    Depositor
    Benjamin Kleinstiver, Keith Joung, Joseph Bondy-Denomy
    Article
    Marino et al Science. 2018 Sep 6. pii: science.aau517
    Insert
    human codon optimized Mb3Cas12a
    Use
    Tags
    NLS(nucleoplasmin) and 3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    115142-plasmid-map-sequence-id-220502

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Showing: 881 - 900 of 4747 results