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Plasmid#162942PurposeGateway attB5r/attB1r entry clone for generation of N-terminal mTFP1 fusion proteinsDepositorInsertmonomeric Teal fluorescent protein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C413-E19: attB4-mPol2p>-attB1r
Plasmid#162971PurposeGateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoterDepositorInsertmurine RNA polymerase 2 promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
C413-E11: attB4-Grp78p>-attB1r
Plasmid#162970PurposeGateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoterDepositorInserthuman Glucose response protein 78 promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
C413-E10: attB4-FerHp>-attB1r
Plasmid#162969PurposeGateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoterDepositorInserthuman Ferritin heavy chain promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
C512-E34: attB5r-iRFP670-nostop-attB1r
Plasmid#162947PurposeGateway attB5r/attB1r entry clone for generation of N-terminal iRFP670 fusion proteinsDepositorInsertfar-red iRFP670 fluorescent protein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
C512-E29: attB5r-mKate2-nostop-attB1r
Plasmid#162946PurposeGateway attB5r/attB1r entry clone for generation of N-terminal mKate2 fusion proteinsDepositorInsertmonomeric Kate2 fluorescent protein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
C512-E15: attB5r-mVenus-nostop-attB1r
Plasmid#162943PurposeGateway attB5r/attB1r entry clone for generation of N-terminal mVenus fusion proteinsDepositorInsertmonomeric Venus fluorescent protein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
C415-E01: attB4-stuffer-attB1r
Plasmid#162931PurposeGateway attB4-attB1r entry clone containing a non-transcribed/translated region for use in generating negative control vectorsDepositorInsertstuffer fragment for vector controls
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
C413-E26: attB4-SV40p>-attB1r
Plasmid#162923PurposeGateway attB4/attB1r entry clone for mammalian expression using the SV40 promoterDepositorInsertSV40 immediate early promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
C413-E21: attB4-PGKp>-attB1r
Plasmid#162921PurposeGateway attB4/attB1r entry clone for mammalian expression using the human PGK promoterDepositorInserthuman pGK promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
C234-E07: attB2r-HA-attB3
Plasmid#162911PurposeGateway attB2r/attB3 entry clone for construction of C-terminal HA epitope tagged fusion proteinsDepositorInsertHA epitope tag
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
C234-E06: attB2r-FLAG-attB3
Plasmid#162910PurposeGateway attB2r/attB3 entry clone for construction of C-terminal FLAG epitope tagged fusion proteinsDepositorInsertFLAG epitope tag
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
C234-E05: attB2r-V5-attB3
Plasmid#162909PurposeGateway attB2r/attB3 entry clone for construction of C-terminal V5 epitope tagged fusion proteinsDepositorInsertV5 epitope tag
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFD-MBP-CS(TEV)-StrepTag-II-AI(TVMV)
Plasmid#137110PurposeiFLinkC Linker cloningDepositorInsertMBP-CS(TEV)-StrepTag-II-AI(TVMV)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C2616
Plasmid#91090PurposeModule C, Promoter: At7SL, Gene: BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol IIIDepositorInsertBsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
UseCRISPRTagsExpressionMutationPromoterAt7SLAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR P2R-P3-V5-6xHis
Plasmid#48347PurposeContributes the coding sequence of a V5-6xHis epitope tags cassette as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertV5 and 6xHis epitope tags cassette
UseGateway entry cloneTagsExpressionMutationContains a stop codon at the end of the epitope t…PromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C2518
Plasmid#91087PurposeModule C, Promoter: TaU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol IIIDepositorInsertEsp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
UseCRISPRTagsExpressionMutationPromoterTaU6Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C2515
Plasmid#91083PurposeModule C, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol IIIDepositorInsertEsp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
UseCRISPRTagsExpressionMutationPromoterAtU6Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C2517
Plasmid#91086PurposeModule C, Promoter: TaU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol IIIDepositorInsertEsp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
UseCRISPRTagsExpressionMutationPromoterTaU3Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C2520
Plasmid#91089PurposeModule C, Promoter: OsU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol IIIDepositorInsertEsp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
UseCRISPRTagsExpressionMutationPromoterOsU6Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only