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pBS-KS-attB1-2-PT-SA-SD-0-mTagBFP2(11x7)

Plasmid

#172064

Purpose

Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 0 coding intron

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

mTagBFP2(11x7)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pBS-KS-attB1-2-PT-SA-SD-1-mTagBFP2(11x7)

Plasmid

#172065

Purpose

Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 1 coding intron

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

mTagBFP2(11x7)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCZGY1584 Pcol-19::ebp-2::mGFP::unc-54 3'UTR

Plasmid

#129774

Purpose

Marker of microtubule dynamics in C. elegans hypodermis

Depositor

Andrew Chisholm

Article

Chuang et al Elife. 2016 Sep 23;5. doi: 10.7554/eLife

Inserts

Pcol-19
ebp-2 (ebp-2 Nematode)
unc-54 3'UTR

Use

Synthetic Biology; Gateway

Tags

Synthetic Intron and mGFP

Expression

Worm

Mutation

Promoter

Pcol-19

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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NFATc2-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75234

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc2 (1/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc2 (NFATC2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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NFATc1-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75238

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc1 (1/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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Helios-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75246

Purpose

CRISPR/Cas9 NICKASE plasmid against human Helios (1/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against human Helios (IKZF2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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Ikaros-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75242

Purpose

CRISPR/Cas9 NICKASE plasmid against human Ikaros (1/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against human Ikaros (IKZF1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE

Plasmid

#180280

Purpose

The CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

CasMINI sgRNA (backbone) and BFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-2/3-3'

Plasmid

#117325

Purpose

CRISPR-Cas9 for miR-124-2/3, 3'

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

gRNA miR-124-2/3-3' (MIR124-3 Synthetic)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-1/2-5'

Plasmid

#117322

Purpose

CRISPR-Cas9 for miR-124-1/2, 5'

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

gRNA miR-124-1/2-5' (MIR124-1 Synthetic)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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-962 human cyclin D1 promoter TCF (1)(2) sites mutant pGL3Basic

Plasmid

#32734

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 promoter (CCND1 Human)

Use

Luciferase

Tags

Expression

Mutation

-75 TCF(1) and -68 TCF(2) - CTTTGATCTTTGCT deleti…

Promoter

-962 CCND1

Available since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
Thy-1-CRISPR-gRNA#2-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124284

Purpose

sgRNA against human Thy-1

Depositor

Csaba Vizler, Katalin Josvay

Insert

Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only
pAV-U6+27-Tornado-F30-Broccoli-Squash-SAM-sensor 4-2

Plasmid

#177919

Purpose

Expresses ratiometric Squash-SAM sensor 4-2 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

Insert

Tornado-F30-Broccoli-Squash-SAM-sensor 4-2

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6+27

Available since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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TL1 alphaDdCBE A1 (DddA6-C) - TL1 alphaL_DdCBE 2 (left, DddA11-C)

Plasmid

#221994

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE A1 repeats-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

TALE: Q231R, W232G, S233A | DddA: T1413I

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ9834_sglacZ: pHR-hU6-CasMINI sgRNA_#2 (sgLacZ); EF1a-Puro-T2A-BFP- WPRE

Plasmid

#176272

Purpose

Expression of non-targeting sgRNA of CasMINI

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

CasMINI non-targeting sgRNA (sgLacZ) and BFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pSLQ9834_sgTet: pHR-hU6-CasMINI sgRNA_#2 (sgTet); EF1a-Puro-T2A-BFP- WPRE

Plasmid

#176273

Purpose

Expression of CasMINI sgRNA targeting TRE3G promoter in dCasMINI-mediated GFP activation assays.

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

CasMINI sgRNA (sgTet) and BFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pTM-RBDv2

Plasmid

#162785

Purpose

SARS-CoV-2 S protein receptor binding domain (RBD) expression in mammalian cells

Depositor

Ivan Vorobiev

Article

Sinegubova et al PLoS One. 2021 Feb 2;16(2):e0242890. doi

Insert

SARS-CoV-2 S protein receptor binding domain (RBD) (S Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2))

Use

Tags

10xHis, c-myc, and hTPA leader

Expression

Mammalian

Mutation

Promoter

CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac1-Nucleocapsid

Plasmid

#179696

Purpose

Expresses H6 tagged SARS CoV-2 nucleocapsid in Sf9 cells

Depositor

Mark Arbing

Insert

SARS-CoV-2 Nucleocapsid (N protein) (N Severe acute respiratory syndrome coronavirus 2)

Use

Tags

His6-TEV protease site

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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Venus-Bcl2-pEGFP-C1

Plasmid

#177417

Purpose

To express Venus fused to the N-terminus of the Bcl-2 family protein, Bcl-2.

Depositor

David Andrews

Article

Aranovich et al Mol Cell. 2012 Mar 30;45(6):754-63. doi:

Insert

Bcl-2 (BCL2 Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-Bcl2-Maob

Plasmid

#18005

Depositor

Clark Distelhorst

Article

Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

Insert

Mitochondria-targeted Bcl-2 (BCL2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

C terminal of Bcl-2 replaced with Maob. Targets B…

Promoter

CMV

Available since

June 4, 2008

Availability

Academic Institutions and Nonprofits only