-
Plasmid#104218PurposeBacterial expression of WW domain #3 from ITCHDepositorInsertITCH WW domain #3 (ITCH Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 ITCH WW domain #4
Plasmid#104219PurposeBacterial expression of WW domain #4 from ITCHDepositorInsertITCH WW domain #4 (ITCH Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 ITCH WW domain #1
Plasmid#104216PurposeBacterial expression of WW domain #1 from ITCHDepositorInsertITCH WW domain #1 (ITCH Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
mCherry-hALIX
Plasmid#21504DepositorInsertALIX (PDCD6IP Human)
UseTagsCherryExpressionBacterialMutationnonePromoterAvailable sinceSept. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-A20
Plasmid#22141DepositorInsertA20 (TNFAIP3 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCBFD1/HBFD1Bad
Plasmid#53364PurposeWill convert beta-carotene into astaxanthin in E. coli when expression of the hbfd1 cDNA is induced with arabinose. Use with pAC-BETA or pAC-BETAipi.DepositorInsertcbfd1
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGST2-ALIX (795-811)
Plasmid#21502DepositorInsertALIX (PDCD6IP Human)
UseTagsGSTExpressionBacterialMutationnonePromoterAvailable sinceSept. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-A20 (OTU, aa 1-366)
Plasmid#61584PurposeExpresses human A20 (OTU domain) in E. coli.DepositorInsertA20 (TNFAIP3 Human)
UseTagsGST-3CExpressionBacterialMutationDeleted aa 367-790.PromotertacAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGST2-Alix (360-702)
Plasmid#17639DepositorInsertAlix (360-702) (PDCD6IP Human)
UseTagsGSTExpressionBacterialMutationAlix V domainPromoterAvailable sinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
5C7M (ITCH)
Plasmid#69966PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertITCH:SDC094-D06:C29524 (ITCH Human)
UseTagsHis and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacOAvailable sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
-
pCellFree_G03 WWP1
Plasmid#67125PurposeStandard for cell-free expression benchmarking.DepositorInsertWWP1 (WWP1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
ALIX-mNeonGreen
Plasmid#191191PurposeFull-length ALIX with C-terminal mNeonGreen tag.DepositorInsertALIX (PDCD6IP Human, Synthetic)
UseTags6xHIS and mNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
p6592 MSCV-IP N-HAonly WWP2
Plasmid#34905DepositorInsertWWP2 (WWP2 Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterMSCV LTRAvailable sinceFeb. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pY2F
Plasmid#53285PurposeContains an Arabidopsis thaliana lcyE cDNA and produces an active lycopene epsilon-cyclase enzyme in E. coli. Use with pAC-BETA-At or pAC-BETAipi-At to produce alpha-carotene in E. coli.DepositorInsertlcyE (LUT2 Mustard Weed)
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
ALIX-His(1-868, P801G)
Plasmid#186808PurposeCodon-optimized bacterial expression plasmid. Expresses ALIX-His(1-868, P801G).DepositorInsertALIX-His (1-868, P801G) (PDCD6IP Human)
UseTagsHis6ExpressionBacterialMutationProline 801 to GlycinePromoterAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAtCHYb1Δ129N
Plasmid#53367PurposeContains a truncated Arabidopsis beta-carotene hydroxylase cDNA, the product of which converts beta-carotene primarily to monocyclic beta-cryptoxanthin in E. coli. Use with pAC-BETA or pAC-BETAipi.DepositorInsertchyB1 (BETA-OHASE 1 Mustard Weed)
UseTagsExpressionBacterialMutationDeleted codons for the first 129 amino acidsPromoterTrcAvailable sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTRdeltaGFPmirGE-DroshaT1.5.5
Plasmid#61084PurposepENTR plasmid containing a mutated GFP followed by 3 miRNA haipins: recommended as backbone.DepositorInsertdeltaGFP
UseRNAiTagsExpressionMutationPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PDCD6IP_BRO1
Plasmid#109994PurposeProtein expression and purification of PDCD6IP_BRO1DepositorInsertPDCD6IP_BRO1 (PDCD6IP Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only