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Showing: 881 - 900 of 2911 results

  11. pB80-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11

    Plasmid
    #174645
    Purpose
    Optogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomes
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)-P2A-ILID-mCherry-RAB11
    Use
    Tags
    SSPB(micro)-Venus and iLID-mCherry
    Expression
    Mammalian
    Mutation
    SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    174645-plasmid-map-sequence-id-347047

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  15. pB80-KIF1A(1-383)-GFP-SSPB(nano)

    Plasmid
    #174628
    Purpose
    Constitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(nano)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-383)-GFP-SSPB(nano) (Kif1a Mouse, Synthetic)
    Use
    Tags
    GFP-SSPB(nano)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-383): Pro202Ala; EGFP: Met1Del;
    Promoter
    Chicken beta-actin
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174628-plasmid-map-sequence-id-346988

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  16. pEGFP-PH-SWAP70(R223E, R224E)

    Plasmid
    #84583
    Purpose
    Expresses GFP-tagged PH-domain of SWAP70 in mammalian cells, impaired phosphoinositide binding
    Depositor
    Geert van den Bogaart
    Article
    Baranov et al Cell Rep. 2016 Nov 1;17(6):1518-1531. do
    Insert
    SWAP70 residues 209-306 with mutations R223E and R224E (SWAP70 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Pleckstrin-homology domain, residues 209-306, mut…
    Promoter
    CMV
    Available since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  17. pB80-KIF1A(1-365)-GFP-SSPB(micro)

    Plasmid
    #174629
    Purpose
    Non-processive monomeric KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-GFP-SSPB(micro) (Kif1a Mouse, Synthetic)
    Use
    Tags
    GFP-SSPB(micro)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; EGFP: Met1Del; SSPB:…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174629-plasmid-map-sequence-id-346983

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Showing: 881 - 900 of 2911 results